Establishment of Orthotopic Liver Transplantation Model in Rats Using Modified Technique by Single Operator_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SHEN Haoyuan ^1,2 , WANG Wei ¹ , LIU Meng ¹ , LI Feng ¹ , JIANG Kaiwen. ¹

1.Department of Hepatobiliary Surgery, The First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, Guangdong Province, China;;
2.Department of General Surgery, The Central Hospital of Xiaogan City, Xiaogan 432100, Hubei Province, China;

Corresponding author：

Keywords：

Orthotopic liver transplantation; Rat; Animal model; Modification; Single operator

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ObjectiveTo explore the surgical techniques of rat orthotopic liver transplantation (OLT) model in rats using modified technique by single operator. MethodsA total of two hundreds cases of rats (one hundred pairs) were used to establish OLT model including eighty cases of rats (forty pairs) used in formal study by using modified Kamada “two-cuff” method, of which the key surgical procedures were further modified. Intraoperative results and postoperative recovery were analyzed. ResultsAll eighty cases of rats successfully operated. Operative time in donors was (28.5±2.4) min and reparative time of donor liver was (10.2±1.8) min. Anastomosis time for suprahepatic inferior vena cava and portal vein (15.3±1.9) min and (3.4±1.2) min, respectively. Anhepatic phase of transplant recipients was (23.8±1.9) min, anastomosis time for infrahepatic inferior vena cava was (5.1±2.1) min, and biliary reconstruction time was (3.1±0.9) min. Blood loss of donor and recipients during the operation was blew 0.5 ml. No recipient died for operation. The 2 d survival rate of recipients was 90% (36/40), three rats died of bleeding at the seam in hepatic inferior vena cava and one rat died of longer anhepatic phase. The 7 d survival rate of recipients was 82.5% (33/40), three rats died of intra-abdominal infections. ConclusionThe modification in en bloc separation of abdominal aorta and interior vena cava for abdominal aortic perfusion of donor liver, careful dissection of left subphrenic vein triangle and the hemostasis by heat coagulating can reduce operative complications, guarantee the quality of donor liver and improve the survival rate of rats in establishment of OLT model in rats using modified technique by single operator.

Citation： SHEN Haoyuan,WANG Wei,LIU Meng,LI Feng,JIANG Kaiwen.. Establishment of Orthotopic Liver Transplantation Model in Rats Using Modified Technique by Single Operator. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(8): 825-828. doi: Copy

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2.	Wan CD, Cheng R, Wang HB, et al. Immunomodulatory effects of mesenchymal stem cells derived from adipose tissues in a rat orthotopic liver transplantation model [J]. Hepatobiliary Pancreat Dis Int, 2008, 7(1): 2933.
3.	Zhu H, Marco C, Gianfranco F. Early changes of graft function, cytokines and superoxide dismutase serum levels after donor liver denervation and Kupffer cell depletion in a rattorat liver transplantation model [J]. Hepatobiliary Pancreat Dis Int, 2009, 8(2): 152156.
4.	Fujiki M, Esquivel CO, Martinez OM, et al. Induced tolerance to rat liver allografts involves apoptosis of intragraft T cells and the generation of CD4+CD25+FoxP3+ T regulatory cells [J]. Liver Transpl, 2010, 16(2): 14754.
5.	Qin L, Guan HG, Zhou XJ, et al. Blockade of 41BB/41BB ligand interactions prevents acute rejection in rat liver transplantation [J]. Chin Med J, 2010 (Engl), 123(2): 212215.
6.	Tolboom H, Pouw RE, Izamis ML, et al. Recovery of warm ischemic rat liver grafts by normothermic extracorporeal perfusion [J]. Transplantation, 2009, 87(2): 170177.
7.	Lee S, Charters AC, Chandler JG, et al. A technique for orthotopic liver transplantation in the rat [J]. Transplantation, 1973, 16(6): 664669.
8.	Lee S, Charters AC 3rd, Orloff MJ. Simplified technic for orthotopic liver transplantation in the rat [J]. Am J Surg, 1975, 130(1): 3840.
9.	Kamada N, Calne RY. Orthotopic liver transplantation in the rat: Technique using cuff for portal vain anastomosis and biliary drainge [J]. Transplantation, 1979, 28(1): 4750.
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11.	Matevossian E, Doll D, Hüser N, et al. Liver transplantation in the rat: singlecenter experience with technique, longterm survival, and functional and histologic findings [J]. Transplant Proc, 2009, 41(6): 26312636.
12.	李镜锋, 丁焕新, 白建华, 等. 无心跳供肝大鼠肝移植模型的手术操作技巧探讨 [J]. 中国普外基础与临床杂志, 2010, 17(8): 785788.
13.	刘静, 李江, 张升宁, 等. 改良法构建大鼠减体积肝移植模型 [J]. 中国组织工程研究与临床康复, 2010, 14(18): 32523257.
14.	黄新立, 张峰, 王学浩, 等. 建立大鼠部分肝移植动脉化模型的实验研究 [J]. 中国普外基础与临床杂志, 2004, 11(3): 224227.
15.	彭勇, 龚建平, 刘长安, 等. 大鼠原位肝移植模型制作过程中麻醉方法的选择 [J]. 中国普通外科杂志, 2003, 12(9): 673676.
16.	金成, 张培建, 冯敏, 等. 经门静脉灌注法大鼠自体原位肝移植模型的建立 [J]. 中国普外基础与临床杂志, 2009, 16(3): 184186.
17.	张升宁, 白建华, 刘静, 等. 大鼠原位肝移植模型的手术改进及体会 [J]. 肝胆外科杂志, 2008, 16(3): 210213.
18.	丘昶儒, 王炜, 程卫, 等. 预实验中大鼠原位肝移植常见失败原因及对策 [J]. 中国普外基础与临床杂志, 2010, 17(10): 10351038.
19.	王宇, 阚和平, 蒋晓青. 双袖套法大鼠异体原位肝移植模型改进与体会 [J]. 中国临床解剖学杂志, 2010, 28(4): 463466.
20.	Jiang Y, Qiu YD, Gu XP, et al. Rat liver transplantation without preservation of “phrenic ring” using double cuff method [J]. World J Gastroenterol, 2004, 10 (10): 15261527.

1. Kebis A, Kukan M, Grancic P, et al. A novel way of liver preservation improves rat liver viability upon reperfusion [J]. J Zhejiang Univ Sci B, 2007, 8(5): 289295.
2. Wan CD, Cheng R, Wang HB, et al. Immunomodulatory effects of mesenchymal stem cells derived from adipose tissues in a rat orthotopic liver transplantation model [J]. Hepatobiliary Pancreat Dis Int, 2008, 7(1): 2933.
3. Zhu H, Marco C, Gianfranco F. Early changes of graft function, cytokines and superoxide dismutase serum levels after donor liver denervation and Kupffer cell depletion in a rattorat liver transplantation model [J]. Hepatobiliary Pancreat Dis Int, 2009, 8(2): 152156.
4. Fujiki M, Esquivel CO, Martinez OM, et al. Induced tolerance to rat liver allografts involves apoptosis of intragraft T cells and the generation of CD4+CD25+FoxP3+ T regulatory cells [J]. Liver Transpl, 2010, 16(2): 14754.
5. Qin L, Guan HG, Zhou XJ, et al. Blockade of 41BB/41BB ligand interactions prevents acute rejection in rat liver transplantation [J]. Chin Med J, 2010 (Engl), 123(2): 212215.
6. Tolboom H, Pouw RE, Izamis ML, et al. Recovery of warm ischemic rat liver grafts by normothermic extracorporeal perfusion [J]. Transplantation, 2009, 87(2): 170177.
7. Lee S, Charters AC, Chandler JG, et al. A technique for orthotopic liver transplantation in the rat [J]. Transplantation, 1973, 16(6): 664669.
8. Lee S, Charters AC 3rd, Orloff MJ. Simplified technic for orthotopic liver transplantation in the rat [J]. Am J Surg, 1975, 130(1): 3840.
9. Kamada N, Calne RY. Orthotopic liver transplantation in the rat: Technique using cuff for portal vain anastomosis and biliary drainge [J]. Transplantation, 1979, 28(1): 4750.
10. Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat [J]. Surgery, 1983, 93(1 Pt 1): 6469.
11. Matevossian E, Doll D, Hüser N, et al. Liver transplantation in the rat: singlecenter experience with technique, longterm survival, and functional and histologic findings [J]. Transplant Proc, 2009, 41(6): 26312636.
12. 李镜锋, 丁焕新, 白建华, 等. 无心跳供肝大鼠肝移植模型的手术操作技巧探讨 [J]. 中国普外基础与临床杂志, 2010, 17(8): 785788.
13. 刘静, 李江, 张升宁, 等. 改良法构建大鼠减体积肝移植模型 [J]. 中国组织工程研究与临床康复, 2010, 14(18): 32523257.
14. 黄新立, 张峰, 王学浩, 等. 建立大鼠部分肝移植动脉化模型的实验研究 [J]. 中国普外基础与临床杂志, 2004, 11(3): 224227.
15. 彭勇, 龚建平, 刘长安, 等. 大鼠原位肝移植模型制作过程中麻醉方法的选择 [J]. 中国普通外科杂志, 2003, 12(9): 673676.
16. 金成, 张培建, 冯敏, 等. 经门静脉灌注法大鼠自体原位肝移植模型的建立 [J]. 中国普外基础与临床杂志, 2009, 16(3): 184186.
17. 张升宁, 白建华, 刘静, 等. 大鼠原位肝移植模型的手术改进及体会 [J]. 肝胆外科杂志, 2008, 16(3): 210213.
18. 丘昶儒, 王炜, 程卫, 等. 预实验中大鼠原位肝移植常见失败原因及对策 [J]. 中国普外基础与临床杂志, 2010, 17(10): 10351038.
19. 王宇, 阚和平, 蒋晓青. 双袖套法大鼠异体原位肝移植模型改进与体会 [J]. 中国临床解剖学杂志, 2010, 28(4): 463466.
20. Jiang Y, Qiu YD, Gu XP, et al. Rat liver transplantation without preservation of “phrenic ring” using double cuff method [J]. World J Gastroenterol, 2004, 10 (10): 15261527.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Establishment of Orthotopic Liver Transplantation Model in Rats Using Modified Technique by Single Operator

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