Effect of Somatostatin Combined with Antibiotics on Serum Tumor Necrosis Factor-α and C-reactive Protein in Rabbits with Acute Cholecystitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Zhituo , DU Jianbo , HAN Bing , HOU Limin.

Department of Emergency Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China;

Corresponding author：

Keywords：

Somatostatin; Tumor necrosis factor-α; Creactive protein; Acute cholecystitis; Antibiotics

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo explore the therapeutic effect and its possible mechanisms of somatostatin combined with antibiotics on acute cholecystitis through the detection of serum tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) in rabbits. MethodsForty-five rabbits were randomly averagely classified into three groups following the establishment of acute cholecystitis model: control group, blank group， and experimental group. The rabbits in control group received cefazolin sodium and metronidazoie by intravenous injection twice a day. The rabbits in experimental group got a hypodermic injection of somatostin (20 μg/kg) twice a day besides antibiotics, while these drugs were replaced by equal volume of normal saline for the rabbits in control group. The concentrations of serum TNFα and CRP were detected by enzyme-linked immunosorbent assay and histomorphological and electron microscopic changes of gallbladder in rabbits were observed on 3 d after administer. ResultsThe concentrations of serum TNF-α of rabbits in experimental group 〔(401.6±48.7) pg/ml〕 were significantly lower than those in control group 〔(767.3±67.4) pg/ml〕 and blank group 〔(806.7±61.2) pg/ml〕, P=0.000 and P=0.000, while the difference between the latter two groups was not significant (P=0.196). The concentrations of serum CRP of rabbits in experimental group 〔(16.2±1.1) mg/L〕 were significantly lower than those in control group 〔(55.4±1.2) mg/L〕 and blank group 〔(72.8±8.9) mg/L〕, P=0.000 and P=0.000, and which was higher in blank group compared with control group (P=0.018). The Histopathological results showed that gallbladder wall emerged mulifocality mucosal fluid necrosis, lamina propia hyperemia, bulk neutrophil infiltration and sequent alleviation of reaction in the rabbits of experimental group when compared with the rabbits of blank group and control group. Electron microscopic results demonstrated that the intercellular junction of gallbladder kept relative integrity and the swelling and vacuolar degeneration of mitochondria and endoplasmic reticulum obviously relieved. ConclusionsSomatostatin can significantly reduce the concentrations of serum TNF-α and CRP in the model of rabbits acute cholecystitis, which may protect the mucous membrane of gallbladder from the inflammation reaction.

Citation： LI Zhituo,DU Jianbo,HAN Bing,HOU Limin.. Effect of Somatostatin Combined with Antibiotics on Serum Tumor Necrosis Factor-α and C-reactive Protein in Rabbits with Acute Cholecystitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(8): 835-839. doi: Copy

1.	Omata F, Deshpande G, Tokuda Y, et al. Metaanalysis: somatostatin or its longacting analogue, octreotide, for prophylaxis against postERCP pancreatitis [J]. J Gastroenterol, 2010, 45(8): 885895.
2.	Chen P, Hu B, Tan Q, et al. Role of neurocrine somatostatin on sphincter of Oddi contractility and intestinal ischemia reperfusioninduced acute pancreatitis in macaques [J]. Neurogastroenterol Motil, 2010, 22(8): 935941.
3.	Weber C, Zulian GB. Malignant irreversible intestinal obstruction: the powerful association of octreotide to corticosteroids, antiemetics, and analgesics [J]. Am J Hosp Palliat Care, 2009, 26(2): 8488.
4.	Ripamonti CI, Easson AM, Gerdes H. Management of malignant bowel obstruction [J]. Eur J Cancer, 2008, 44(8): 11051115.
5.	郭鹞. 人类疾病的动物模型 [M]. 北京: 人民卫生出版社, 1982: 302305.
6.	田永立, 李家邦. 清肝利湿汤治疗胆囊炎胆石症的实验研究 [J]. 湖南中医学院学报, 1999, 19(3): 57, 9.
7.	陶兴和. 急性胆囊炎患者胆汁细菌培养及药物敏感试验分析 [J]. 中国全科医学, 2009, 12(6B): 10861087.
8.	胡蓉, 盛宇伟, 王传海. 198例急性胆囊炎手术胆汁需氧菌培养与抗生素选用临床研究 [J]. 中国药房, 2008, 19(29): 22742276.
9.	陈美华, 王瑶瑶, 庞桂兰. 128例急性胆囊炎患者胆汁细菌培养分析 [J]. 医学综述, 2009, 15(20): 31853187.
10.	张永杰. 胆囊、胆总管结石患者与对照者胆囊胆汁及胆总管胆汁的同步细菌学检测分析 [J]. 国外医学外科学分册, 1996, 23(6): 370371.
11.	Opal SM. Tumor necrosis factor receptor expression on inflammatory cells in sepsis [J]. Crit Care Med, 1999, 27(2): 240241.
12.	Maier B, Lehnert M, Laurer HL, et al. Delayed elevation of soluble tumor necrosis factor receptors p75 and p55 in cerebrospinal fluid and plasma after traumatic brain injury [J]. Shock, 2006, 26(2): 122127.
13.	徐延钧, 吴新民, 郭亚民. 肿瘤坏死因子α及白介素在急性胰腺炎中的作用 [J]. 中国普外基础与临床杂志, 2010, 17(9): 993996.
14.	Brunkhorst FM. Sepsismarker—what is useful? [J]. Dtsch Med Wochenschr， 2008, 133(48): 25122515.
15.	Szalai AJ. The biological functions of Creactive protein [J]. Vascul Pharmacol, 2002, 39(3): 105107.
16.	Ohtani T, Hirayama A, Kodama K. Clinical value of biochemical markers in patients with unstable angina [J]. Nippon Rinsho, 2003, 61 Suppl 5: 200205.
17.	Gould JM, Weiser JN. The inhibitory effect of Creactive protein on bacterial phosphorylcholine plateletactivating factor receptormediated adherence is blocked by surfactant [J]. J Infect Dis, 2002, 186(3): 361371.
18.	Tilg H, Vannier E, Vachino G, et al. Antiinflammatory properties of hepatic acute phase proteins: preferential induction of interleukin 1 (IL1) receptor antagonist over IL1 beta synthesis by human peripheral blood mononuclear cells [J]. J Exp Med, 1993, 178(5): 16291636.
19.	Ballou SP, Lozanski G. Induction of inflammatory cytokine release from cultured human monocytes by Creactive protein [J]. Cytokine, 1992, 4(5): 361368.
20.	刘晓毅, 杨林, 祁光裕, 等. 白介素、C反应蛋白和纤维蛋白原在下肢深静脉血栓炎症反应中的作用 [J]. 中国普外基础与临床杂志, 2010, 17(7): 660663.
21.	Burdon D, Zabel P. Acute phase reaction and immunocompetence in sepsis and SIRS [J]. Wien Klin Wochenschr, 2002, 114 Suppl 1: 18.
22.	Munford RS, Pugin J. Normal responses to injury prevent systemic inflammation and can be immunosuppressive [J]. Am J Respir Crit Care Med, 2001, 163(2): 316321.
23.	张颖, 于学忠. 急性重症胰腺炎患者生长抑素干预前后pHi监测及其临床意义 [J]. 中国危重急救医学, 2002, 14(6): 368370.
24.	吴硕东, 张振海, 金俊哲,等. 影响奥狄括约肌运动功能药物的胆道镜测压研究 [J]. 中华医学杂志, 2005, 85(27): 19111915.
25.	李延钧, 吴硕东. 生长抑素对Oddi’s括约肌功能的影响 [J]. 肝胆胰外科杂志, 2005, 17(2): 126128.

1. Omata F, Deshpande G, Tokuda Y, et al. Metaanalysis: somatostatin or its longacting analogue, octreotide, for prophylaxis against postERCP pancreatitis [J]. J Gastroenterol, 2010, 45(8): 885895.
2. Chen P, Hu B, Tan Q, et al. Role of neurocrine somatostatin on sphincter of Oddi contractility and intestinal ischemia reperfusioninduced acute pancreatitis in macaques [J]. Neurogastroenterol Motil, 2010, 22(8): 935941.
3. Weber C, Zulian GB. Malignant irreversible intestinal obstruction: the powerful association of octreotide to corticosteroids, antiemetics, and analgesics [J]. Am J Hosp Palliat Care, 2009, 26(2): 8488.
4. Ripamonti CI, Easson AM, Gerdes H. Management of malignant bowel obstruction [J]. Eur J Cancer, 2008, 44(8): 11051115.
5. 郭鹞. 人类疾病的动物模型 [M]. 北京: 人民卫生出版社, 1982: 302305.
6. 田永立, 李家邦. 清肝利湿汤治疗胆囊炎胆石症的实验研究 [J]. 湖南中医学院学报, 1999, 19(3): 57, 9.
7. 陶兴和. 急性胆囊炎患者胆汁细菌培养及药物敏感试验分析 [J]. 中国全科医学, 2009, 12(6B): 10861087.
8. 胡蓉, 盛宇伟, 王传海. 198例急性胆囊炎手术胆汁需氧菌培养与抗生素选用临床研究 [J]. 中国药房, 2008, 19(29): 22742276.
9. 陈美华, 王瑶瑶, 庞桂兰. 128例急性胆囊炎患者胆汁细菌培养分析 [J]. 医学综述, 2009, 15(20): 31853187.
10. 张永杰. 胆囊、胆总管结石患者与对照者胆囊胆汁及胆总管胆汁的同步细菌学检测分析 [J]. 国外医学外科学分册, 1996, 23(6): 370371.
11. Opal SM. Tumor necrosis factor receptor expression on inflammatory cells in sepsis [J]. Crit Care Med, 1999, 27(2): 240241.
12. Maier B, Lehnert M, Laurer HL, et al. Delayed elevation of soluble tumor necrosis factor receptors p75 and p55 in cerebrospinal fluid and plasma after traumatic brain injury [J]. Shock, 2006, 26(2): 122127.
13. 徐延钧, 吴新民, 郭亚民. 肿瘤坏死因子α及白介素在急性胰腺炎中的作用 [J]. 中国普外基础与临床杂志, 2010, 17(9): 993996.
14. Brunkhorst FM. Sepsismarker—what is useful? [J]. Dtsch Med Wochenschr， 2008, 133(48): 25122515.
15. Szalai AJ. The biological functions of Creactive protein [J]. Vascul Pharmacol, 2002, 39(3): 105107.
16. Ohtani T, Hirayama A, Kodama K. Clinical value of biochemical markers in patients with unstable angina [J]. Nippon Rinsho, 2003, 61 Suppl 5: 200205.
17. Gould JM, Weiser JN. The inhibitory effect of Creactive protein on bacterial phosphorylcholine plateletactivating factor receptormediated adherence is blocked by surfactant [J]. J Infect Dis, 2002, 186(3): 361371.
18. Tilg H, Vannier E, Vachino G, et al. Antiinflammatory properties of hepatic acute phase proteins: preferential induction of interleukin 1 (IL1) receptor antagonist over IL1 beta synthesis by human peripheral blood mononuclear cells [J]. J Exp Med, 1993, 178(5): 16291636.
19. Ballou SP, Lozanski G. Induction of inflammatory cytokine release from cultured human monocytes by Creactive protein [J]. Cytokine, 1992, 4(5): 361368.
20. 刘晓毅, 杨林, 祁光裕, 等. 白介素、C反应蛋白和纤维蛋白原在下肢深静脉血栓炎症反应中的作用 [J]. 中国普外基础与临床杂志, 2010, 17(7): 660663.
21. Burdon D, Zabel P. Acute phase reaction and immunocompetence in sepsis and SIRS [J]. Wien Klin Wochenschr, 2002, 114 Suppl 1: 18.
22. Munford RS, Pugin J. Normal responses to injury prevent systemic inflammation and can be immunosuppressive [J]. Am J Respir Crit Care Med, 2001, 163(2): 316321.
23. 张颖, 于学忠. 急性重症胰腺炎患者生长抑素干预前后pHi监测及其临床意义 [J]. 中国危重急救医学, 2002, 14(6): 368370.
24. 吴硕东, 张振海, 金俊哲,等. 影响奥狄括约肌运动功能药物的胆道镜测压研究 [J]. 中华医学杂志, 2005, 85(27): 19111915.
25. 李延钧, 吴硕东. 生长抑素对Oddi’s括约肌功能的影响 [J]. 肝胆胰外科杂志, 2005, 17(2): 126128.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Somatostatin Combined with Antibiotics on Serum Tumor Necrosis Factor-α and C-reactive Protein in Rabbits with Acute Cholecystitis

Abstract Full text Figures/Tables Video References Cited by

Format

Content