Effects of Carbon Dioxide Pneumoperitoneum on Serum Levels of IL-1, IL-6, and CD11a/CD18 and CD11b/CD18 Expression and Pancreatic Pathology in Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HE Hongjie , QIN Chunhong , ZHANG Xuelin , WU Jiang

Department of General Surgery, The Second Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China;

Corresponding author：

Keywords：

Severe acute pancreatitis; CO2 pneumoperitoneum; Interleukin; Dissolubility adhesion molecule; Rat

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To examine the effects of carbon dioxide (CO2) pneumoperitoneum on local pancreas pathological changes, serum levels of amylase, IL-1, IL-6, and the positive rate of dissolubility adhesion molecule (CD11a/CD18 and CD11b/CD18) expression in rats with severe acute pancreatitis (SAP).
Methods Fifty healthy male SpragueDawley (SD) rats were randomly divided into 3 groups: CO2 pneumoperitoneum group (n=20): SAP was induced by injecting 5% sodium taurocholate through retrogradely common biliopancreatic ducts via duodenal papilla， and then CO2 pneumoperitoneum was established at a pressure of 12 mm Hg (1 mm Hg=0.133 kPa) for 30 min; SAP group (n=20): The rats were treated as same as CO2 pneumoperitoneum group, except CO2 pneumoperitoneum； Simple operation group (n=10): Laparotomy was performed and nothing was done to duodenum and pancreas except for moving them softly. The blood samples were collected for examining serum levels of amylase, IL-1, IL-6, and the positive rates of CD11a/CD18 and CD11b/CD18 expression, and histopathologic examination of pancreas was performed.
Results Compared with simple operation group, the pancreatic pathologic histology score, serum levels of amylase, IL-1, IL-6, and the positive rates of CD11a/CD18 and CD11b/CD18 expression were significantly higher in CO2 pneumoperitoneum group and SAP group (P=0.000). The levels of IL-1 and IL-6 were significantly lower in CO2 pneumoperitoneum group as compared to SAP group (P=0.000). There was no significant difference between CO2 pneumoperitoneum group and SAP group in pancreatic pathologic histology score (P=0.294), the level of serum amylase (P=0.073), the positive rates of CD11a/CD18 (P=0.155) and CD11b/CD18 expression (P=0.201).
Conclusion CO2 pneumoperitoneum has inhibitory effect on the levels of IL-1 and IL-6, rather than the positive rates of CD11a/CD18 and CD11b/CD18 expression in SD rats with SAP.

Citation： HE Hongjie,QIN Chunhong,ZHANG Xuelin,WU Jiang. Effects of Carbon Dioxide Pneumoperitoneum on Serum Levels of IL-1, IL-6, and CD11a/CD18 and CD11b/CD18 Expression and Pancreatic Pathology in Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(2): 147-151. doi: Copy

1.	张圣道，雷若庆. 指南后时代重症急性胰腺炎外科治疗的一些关键问题 [J]. 中国普外基础与临床杂志, 2007; 14(4): 381382.
2.	Zhu JF, Fan XH, Zhang XH. Laparoscopic treatment of severe acute pancreatitis [J]. Surg Endosc, 2001; 15(10): 12391241.
3.	黄克俭, 花人放, 孔今城, 等. 静脉输注脂肪乳剂对急性坏死性胰腺炎大鼠脂质炎性介质的影响 [J]. 上海医学, 1998; 21(12): 700702.
4.	Yol S, Bostanci EB, Ozogul Y, et al. Effect of carbon dioxide pneumoperitoneum on the severity of acute pancreatitis: an experimental study in rats [J]. Surg Endosc, 2004; 18(12): 17471751.
5.	Schmidt J, Lewandrowski K, Fernandezdel Castillo C, et al. Histopathologic correlates of serum amylase activity in acute experimental pancreatitis [J]. Dig Dis Sci, 1992; 37(9): 14261433.
6.	Mofidi R, Patil PV, Suttie SA, et al. Risk assessment in acute pancreatitis [J]. Br J Surg， 2009; 96(2): 137150.
7.	余文，吴伟，张少辉，等. 急性胰腺炎发病机理的研究进展 [J]. 中国普外基础与临床杂志, 2006; 13(6): 733736.
8.	Bhatia M. Acute pancreatitis as a model of SIRS [J]. Front Biosci, 2009; 14: 20422050.
9.	Jiang CF, Shiau YC, Ng KW, et al. Serum interleukin6, tumor necrosis factor alpha and Creactive protein in early prediction of severity of acute pancreatitis [J]. J Chin Med Assoc, 2004; 67(9): 442446.
10.	Alfonsi P, VieillardBaron A, Coggia M,et al. Cardiac function during intraperitoneal CO2 insufflation for aortic surgery: a transesophageal echocardiographic study [J]. Anesth Analg, 2006; 102(5): 13041310.
11.	Ure BM, Niewold TA, Bax NM, et al. Peritoneal, systemic, and distant organ inflammatory responses are reduced by a laparoscopic approach and carbon dioxide versus air [J]. Surg Endosc, 2002; 16(5): 836842.
12.	Champault G, Cruaud P, Guilon P, et al. Is carbon dioxide responsible for the reduction in postoperative infections following laparoscopic surgery? [J]. Eur J Coelio Surg, 1997; 3: 3134.
13.	Kopernik G, Avinoach E, Grossman Y, et al. The effect of a high partial pressure of carbon dioxide environment on metabolism and immune functions of human peritoneal cellsrelevance to carbon dioxide pneumoperitoneum [J]. Am J Obstet Gynecol, 1998; 179 (6 Pt 1): 15031510.
14.	Luk JM, Tung PH, Wong KF, et al. Laparoscopic surgery induced interleukin6 levels in serum and gut mucosa: implications of peritoneum integrity and gas factors [J]. Surg Endosc, 2009; 23(2): 370376.
15.	Novitsky YW, Czerniach DR, Kaban GK, et al. Immunologic effects of handassisted surgery on peritoneal macrophages: comparison to open and standard laparoscopic approaches [J]. Surgery, 2006; 139(1): 3945.
16.	Duerr FM, Twedt DC, Monnet E. Changes in pH of peritoneal fluid associated with carbon dioxide insufflation during laparoscopic surgery in dogs [J]. Am J Vet Res, 2008; 69(2): 298301.
17.	Sun W, Watanabe Y, Wang ZQ. Expression and significance of ICAM1 and its counter receptors LFA1 and Mac1 in experimental acute pancreatitis of rats [J].World J Gastroenterol, 2006; 12(31): 50055009.
18.	Hughes SF, Hendricks BD, Edwards DR, et al. Mild episodes of tourniquetinduced forearm ischaemiareperfusion injury results in leukocyte activation and changes in inflammatory and coagulation markers [J]. J Inflamm (Lond), 2007; 4: 12.
19.	Lan W, Harmon D, Wang JH, et al. The effect of lidocaine on neutrophil CD11b/CD18 and endothelial ICAM1 expression and IL1beta concentrations induced by hypoxiareoxygenation [J]. Eur J Anaesthesiol, 2004; 21(12): 967972.
20.	Zhou MY, Lo SK, Bergenfeldt M, et al. In vivo expression of neutrophil inhibitory factor via gene transfer prevents lipopolysaccharideinduced lung neutrophil infiltration and injury by a beta2 integrindependent mechanism [J]. J Clin Invest, 1998; 101(11): 24272437.

1. 张圣道，雷若庆. 指南后时代重症急性胰腺炎外科治疗的一些关键问题 [J]. 中国普外基础与临床杂志, 2007; 14(4): 381382.
2. Zhu JF, Fan XH, Zhang XH. Laparoscopic treatment of severe acute pancreatitis [J]. Surg Endosc, 2001; 15(10): 12391241.
3. 黄克俭, 花人放, 孔今城, 等. 静脉输注脂肪乳剂对急性坏死性胰腺炎大鼠脂质炎性介质的影响 [J]. 上海医学, 1998; 21(12): 700702.
4. Yol S, Bostanci EB, Ozogul Y, et al. Effect of carbon dioxide pneumoperitoneum on the severity of acute pancreatitis: an experimental study in rats [J]. Surg Endosc, 2004; 18(12): 17471751.
5. Schmidt J, Lewandrowski K, Fernandezdel Castillo C, et al. Histopathologic correlates of serum amylase activity in acute experimental pancreatitis [J]. Dig Dis Sci, 1992; 37(9): 14261433.
6. Mofidi R, Patil PV, Suttie SA, et al. Risk assessment in acute pancreatitis [J]. Br J Surg， 2009; 96(2): 137150.
7. 余文，吴伟，张少辉，等. 急性胰腺炎发病机理的研究进展 [J]. 中国普外基础与临床杂志, 2006; 13(6): 733736.
8. Bhatia M. Acute pancreatitis as a model of SIRS [J]. Front Biosci, 2009; 14: 20422050.
9. Jiang CF, Shiau YC, Ng KW, et al. Serum interleukin6, tumor necrosis factor alpha and Creactive protein in early prediction of severity of acute pancreatitis [J]. J Chin Med Assoc, 2004; 67(9): 442446.
10. Alfonsi P, VieillardBaron A, Coggia M,et al. Cardiac function during intraperitoneal CO2 insufflation for aortic surgery: a transesophageal echocardiographic study [J]. Anesth Analg, 2006; 102(5): 13041310.
11. Ure BM, Niewold TA, Bax NM, et al. Peritoneal, systemic, and distant organ inflammatory responses are reduced by a laparoscopic approach and carbon dioxide versus air [J]. Surg Endosc, 2002; 16(5): 836842.
12. Champault G, Cruaud P, Guilon P, et al. Is carbon dioxide responsible for the reduction in postoperative infections following laparoscopic surgery? [J]. Eur J Coelio Surg, 1997; 3: 3134.
13. Kopernik G, Avinoach E, Grossman Y, et al. The effect of a high partial pressure of carbon dioxide environment on metabolism and immune functions of human peritoneal cellsrelevance to carbon dioxide pneumoperitoneum [J]. Am J Obstet Gynecol, 1998; 179 (6 Pt 1): 15031510.
14. Luk JM, Tung PH, Wong KF, et al. Laparoscopic surgery induced interleukin6 levels in serum and gut mucosa: implications of peritoneum integrity and gas factors [J]. Surg Endosc, 2009; 23(2): 370376.
15. Novitsky YW, Czerniach DR, Kaban GK, et al. Immunologic effects of handassisted surgery on peritoneal macrophages: comparison to open and standard laparoscopic approaches [J]. Surgery, 2006; 139(1): 3945.
16. Duerr FM, Twedt DC, Monnet E. Changes in pH of peritoneal fluid associated with carbon dioxide insufflation during laparoscopic surgery in dogs [J]. Am J Vet Res, 2008; 69(2): 298301.
17. Sun W, Watanabe Y, Wang ZQ. Expression and significance of ICAM1 and its counter receptors LFA1 and Mac1 in experimental acute pancreatitis of rats [J].World J Gastroenterol, 2006; 12(31): 50055009.
18. Hughes SF, Hendricks BD, Edwards DR, et al. Mild episodes of tourniquetinduced forearm ischaemiareperfusion injury results in leukocyte activation and changes in inflammatory and coagulation markers [J]. J Inflamm (Lond), 2007; 4: 12.
19. Lan W, Harmon D, Wang JH, et al. The effect of lidocaine on neutrophil CD11b/CD18 and endothelial ICAM1 expression and IL1beta concentrations induced by hypoxiareoxygenation [J]. Eur J Anaesthesiol, 2004; 21(12): 967972.
20. Zhou MY, Lo SK, Bergenfeldt M, et al. In vivo expression of neutrophil inhibitory factor via gene transfer prevents lipopolysaccharideinduced lung neutrophil infiltration and injury by a beta2 integrindependent mechanism [J]. J Clin Invest, 1998; 101(11): 24272437.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Carbon Dioxide Pneumoperitoneum on Serum Levels of IL-1, IL-6, and CD11a/CD18 and CD11b/CD18 Expression and Pancreatic Pathology in Severe Acute Pancreatitis

Abstract Full text Figures/Tables Video References Cited by

Format

Content