Study on Immune Tolerance of Liver Transplantation Inducted by Pre-Infusion with Allogeneic Lymphocytes Treated by 5-FU in Rats _CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHENG Wei , WANG Wei , QIU Changru , SHEN Haoyuan.

Department of Hepato-Biliary Surgery, The First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, China;

Corresponding author：

Keywords：

Liver transplantation; Rat; Immune tolerance; Lymphocytes

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Objective To investigate the effect of pre-infusion with allogeneic lymphocytes treated by 5-FU on inducting immune tolerance of liver transplantation in rats. Methods Wistar and SD rats were used as liver transplantation donors and recipients, respectively. They were divided into 4 groups as following: control group: liver was transplanted from Wistar to SD rats without any other treatment; lymphocytes group: recipient was pre-infused lymphocytes （5×106 cell/ml, 1 ml） from Wistar rat 7 d and 4 d separately before transplantation; low concentration of 5-FU with lymphocytes group: lymphocytes were treated by 5-FU (7.5 μg) before pre-infusion; high concentration of 5-FU with lymphocytes group: lymphocytes were treated by 5-FU (15 μg) before pre-infusion. Pathological changes were observed on day 7 after liver transplantation. Results Acute slight rejection was observed in low concentration of 5-FU with lymphocytes group: liver cell cords were well-arranged basically, hepatic lobules structures could be observed, a few inflammatory cells infiltrated around central veins, and a few lymphocytes infiltrated around portal area. Acute severe rejection was observed in control group, and acute moderate rejection was observed in high concentration of 5-FU with lymphocytes group and lymphocytes group. Conclusion Pre-infusion of lymphocytes treated with low level 5-FU can induce immune tolerance better in recipients after liver transplantation.

Citation： CHENG Wei,WANG Wei,QIU Changru,SHEN Haoyuan.. Study on Immune Tolerance of Liver Transplantation Inducted by Pre-Infusion with Allogeneic Lymphocytes Treated by 5-FU in Rats . CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(11): 1132-1135. doi: Copy

1.	朱继业, 冷希圣. 肝移植治疗肝癌 [J]. 中国普外基础与临床杂志, 2006; 13(2): 140-141.
2.	Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat [J]. Surgery, 1983; 93(1 Pt 1): 64-69.
3.	秦磊, 高广勇, 余斌, 等. 改良二袖套法大鼠原位肝脏移植150例报道 [J]. 苏州大学学报(医学版), 2005; 25(1): 77-80.
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5.	彭方兴, 郑本波, 刘立新, 等. 大鼠原位肝移植动物模型制作要点 [J]. 中国普外基础与临床杂志, 2005; 12(2): 117-119, 149.
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13.	黄华, 李红春, 潘海斌, 等. 肝移植受体免疫耐受的诱导及CD4+CD25+T细胞的重要作用 [J]. 中国组织工程研究与临床康复, 2009; 13(18): 3455-3458.
14.	Shi J, Gilbert GE, Kokubo Y, et al. Role of the liver in regulating numbers of circulating neutrophils [J]. Blood, 2001; 98(4): 1226-1230.
15.	周天保, 杨广顺. Wistar-SD大鼠原位肝移植急性排斥反应的观察 [J]. 中国普通外科杂志, 2008; 17(7): 641-644.
16.	Starzl TE, Demetris AJ, Trucco M, et al. Cell migration and chimerism after whole-organ transplantation: the basis of graft acceptance [J]. Hepatology, 1993; 17(6): 1127-1152.
17.	Starzl TE, Demetris AJ, Murase N, et al． Cell migration, chimerism, and graft acceptance [J]．Lancet, 1992; 339(8809): 1579-1582.
18.	孙志东, 詹林盛, 王全立. 关于移植后微嵌合体的研究进展 [J]. 北京医学, 2007; 29(6): 371-373.
19.	Starzl TE, Demetris AJ, Murase N, et al. The lost chord: microchimerism and allograft survival [J]. Immunol Today, 1996; 17(12): 577-584.
20.	徐光勇, 张唯力, 张荣贵, 等. FasL基因转染诱导大鼠肾移植免疫耐受的实验研究 [J]. 重庆医科大学学报, 2009; 34（9）: 1162-1165.
21.	张娇, 刘倩. 肝癌Fas/FasL途径免疫逃逸机制及对策 [J]. 中国现代普通外科进展, 2006; 9（2）: 71-73.
22.	纪柏, 王广义, 谭毓铨. Fas/FasL诱导肝脏移植免疫耐受研究进展 [J]. 中华肝胆外科杂志, 2005; 11（4）: 287-288.
23.	李国强, 王学浩, 孙倍成, 等. 转Fas配体基因的树突状细胞在诱导大鼠肝移植免疫耐受中的作用 [J]. 中华器官移植杂志, 2003; 24（1）： 7-10.
24.	Pan TL, Goto S, Lin YC, et al. The fas and fas ligand pathways in liver allograft tolerance [J]. Clin Exp Immunol, 1999; 118(1): 180-187.
25.	葛建军, 内田孝之, 张启伟, 等. 5-氟尿嘧啶诱导人淋巴细胞免疫耐受的作用观察 [J]. 中华微生物学和免疫学杂志, 1999; 19(2): 58-59.

1. 朱继业, 冷希圣. 肝移植治疗肝癌 [J]. 中国普外基础与临床杂志, 2006; 13(2): 140-141.
2. Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat [J]. Surgery, 1983; 93(1 Pt 1): 64-69.
3. 秦磊, 高广勇, 余斌, 等. 改良二袖套法大鼠原位肝脏移植150例报道 [J]. 苏州大学学报(医学版), 2005; 25(1): 77-80.
4. 李涛, 唐华美, 孙星, 等. “二袖套法”大鼠原位肝移植的技术改进 [J]. 中国普通外科杂志, 2005; 14(7): 486-488.
5. 彭方兴, 郑本波, 刘立新, 等. 大鼠原位肝移植动物模型制作要点 [J]. 中国普外基础与临床杂志, 2005; 12(2): 117-119, 149.
6. An International Panel. Banff schema for grading liver allograft rejection: an international consensus document [J]. Hepatology, 1997; 25(3): 658-663.
7. 沈文律, 沈凯, 范丽明. 肝脏移植后排斥反应的病理诊断 [J]. 中国普外基础与临床杂志, 2001; 8(6): 429-432.
8. 孙凌云, 丁义涛. 免疫抑制剂在肝移植中的研究进展 [J]. 肝胆外科杂志, 2000; 8（3）： 239-240.
9. 梁翠萍, 刘华, 李玉, 等. 免疫抑制剂在肝移植术后早期应用的护理 [J]. 现代护理, 2001; 7（2）: 29-30.
10. 吴军. 肝移植后免疫抑制药的应用 [J]. 中国组织工程研究与临床康复, 2009; 13(18): 3495-3498.
11. 黄建钊, 夏穗生. 对肝移植免疫耐受机理研究的思索 [J]. 中国普外基础与临床杂志, 2000; 7(4): 261-262.
12. 韩德恩, 张新晨. 肝移植免疫耐受细胞迁移学说及研究进展 [J]. 哈尔滨医科大学学报, 2001; 35（1）: 69-71.
13. 黄华, 李红春, 潘海斌, 等. 肝移植受体免疫耐受的诱导及CD4+CD25+T细胞的重要作用 [J]. 中国组织工程研究与临床康复, 2009; 13(18): 3455-3458.
14. Shi J, Gilbert GE, Kokubo Y, et al. Role of the liver in regulating numbers of circulating neutrophils [J]. Blood, 2001; 98(4): 1226-1230.
15. 周天保, 杨广顺. Wistar-SD大鼠原位肝移植急性排斥反应的观察 [J]. 中国普通外科杂志, 2008; 17(7): 641-644.
16. Starzl TE, Demetris AJ, Trucco M, et al. Cell migration and chimerism after whole-organ transplantation: the basis of graft acceptance [J]. Hepatology, 1993; 17(6): 1127-1152.
17. Starzl TE, Demetris AJ, Murase N, et al． Cell migration, chimerism, and graft acceptance [J]．Lancet, 1992; 339(8809): 1579-1582.
18. 孙志东, 詹林盛, 王全立. 关于移植后微嵌合体的研究进展 [J]. 北京医学, 2007; 29(6): 371-373.
19. Starzl TE, Demetris AJ, Murase N, et al. The lost chord: microchimerism and allograft survival [J]. Immunol Today, 1996; 17(12): 577-584.
20. 徐光勇, 张唯力, 张荣贵, 等. FasL基因转染诱导大鼠肾移植免疫耐受的实验研究 [J]. 重庆医科大学学报, 2009; 34（9）: 1162-1165.
21. 张娇, 刘倩. 肝癌Fas/FasL途径免疫逃逸机制及对策 [J]. 中国现代普通外科进展, 2006; 9（2）: 71-73.
22. 纪柏, 王广义, 谭毓铨. Fas/FasL诱导肝脏移植免疫耐受研究进展 [J]. 中华肝胆外科杂志, 2005; 11（4）: 287-288.
23. 李国强, 王学浩, 孙倍成, 等. 转Fas配体基因的树突状细胞在诱导大鼠肝移植免疫耐受中的作用 [J]. 中华器官移植杂志, 2003; 24（1）： 7-10.
24. Pan TL, Goto S, Lin YC, et al. The fas and fas ligand pathways in liver allograft tolerance [J]. Clin Exp Immunol, 1999; 118(1): 180-187.
25. 葛建军, 内田孝之, 张启伟, 等. 5-氟尿嘧啶诱导人淋巴细胞免疫耐受的作用观察 [J]. 中华微生物学和免疫学杂志, 1999; 19(2): 58-59.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on Immune Tolerance of Liver Transplantation Inducted by Pre-Infusion with Allogeneic Lymphocytes Treated by 5-FU in Rats 

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