Establishment and Characterization of Human Gastric Cancer Cell Line SGC-7901/HCPT Xenograft in Nude Mice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Pan , WEI Shoujiang , HE Yi , HOU Huafang , WANG Chongshu.

Department of General Surgery, The Affiliated Hospital of North of Sichuan Medical College, Nanchong 637000, Sichuan Province, China;

Corresponding author：

Keywords：

Gastric cancer; Hydroxycamptothecine; Nude mice; Transplantation tumor; Drug resistance

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To establish a xenograft model of hydroxycamptothecine (HCPT)-resistant human gastric cancer cell line (SGC-7901/HCPT) in nude mice and study its biological characteristics. Methods The SGC-7901 and SGC-7901/ HCPT cells were cultured in vitro. The cell suspension was injected subcutaneously into the nude mice. When the subcutaneous carcinoma was 1.0 cm in diameter, it was cut off and divided into pieces of 0.1-0.2 cm in diameter． Then the small pieces of tumor were re-transplanted subcutaneously into the second generation nude mice until the fourth generation. The morphological feature, ultramicro-structure, and growth characteristics of the fourth generation transplanted tumor were examined. The drug resistance was measured by methyl thiazolyl tetrazolium (MTT) assay. Results The transplanted tumor in nude mice was round or oval, and many blood vessels were on its surface. Under the light microscope, the sizes of SGC-7901 transplanted tumor cells were similar, and sizes of cell nuclei were also similar; Meanwhile, the morphous of SGC-7901/HCPT transplanted tumor cells were irregular and in disorder, and the size of the cell nuclei was different from each other. Under the electron microscope, the mitochondria and endoplasmic reticulum of SGC-7901 transplanted tumor cells were nearly normal and no swelling in cell nuclei; Meanwhile the cell nuclei of SGC-7901/HCPT transplanted tumor cells were lightly swelled, a the mitochondria and endoplasmic reticulum were obviously swelled. By MTT assay, compared with SGC-7901 transplanted tumor cells, the resistance index of SGC-7901/HCPT transplanted tumor cells was 9.02±0.78 in HCPT, and resistance index to Adriamycin， Mitomycin C, 5-fluorouracil, and Etoposide was 1.24±0.09, 1.31±0.17, 0.96±0.12, and 1.07±0.16, respectively. Conclusions A transplanted tumor model of SGC-7901/HCPT in nude mice is established successfully， and showing stable drug resistance to HCPT and no cross-resistance to other chemotherapeutics, which can be used for further experiments.

Citation： WANG Pan,WEI Shoujiang,HE Yi,HOU Huafang,WANG Chongshu.. Establishment and Characterization of Human Gastric Cancer Cell Line SGC-7901/HCPT Xenograft in Nude Mice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(11): 1147-1151. doi: Copy

1.	Meyer HJ. The influence of case load and the extent of resection on the quality of treatment outcome in gastric cancer [J]. Eur J Surg Oncol, 2005; 31(6): 595-604.
2.	Sivins A, Pedrazzani C, Roviello F, et al. Surgical treatment of gastric cancer in Latvia: results of centralized experience [J]. Eur J Surg Oncol, 2009; 35(5): 481-485.
3.	Morabito A, Carillio G, Longo R. Systemic treatment of gastric cancer [J]. Crit Rev Oncol Hematol, 2009; 70(3): 216-234.
4.	Cervenak J, Andrikovics H, Ozvegy-Laczka C, et al. The role of the human ABCG2 multidrug transporter and its variants in cancer therapy and toxicology [J]. Cancer Lett, 2006; 234(1): 62-72.
5.	Chun JH, Kim HK, Lee JS, et al. Weekly irinotecan in patients with metastatic gastric cancer failing cisplatin-based chemotherapy [J]. Jpn J Clin Oncol, 2004; 34(1): 8-13.
6.	林芸秀, 吕联煌, 林锦娟, 等. 人小细胞肺癌NCI-H446细胞裸鼠异种移植瘤模型的建立 [J]. 实验动物科学与管理, 2004; 21(3): 1-3.
7.	何一, 王崇树, 魏寿江, 等. 人胃癌耐羟基喜树碱细胞株SGC-7901/HCPT 的建立及其生物学性状 [J]. 中国现代医生, 2008; 46(3): 15-17.
8.	Branimir IS. Natural and Acquired Resistance to Cancer Therapies [A]. In: John Mendelsohn MD, Peter M. Howley MD, Mark A. Israel MD, et al. The Molecular Basis of Cancer [M]. Third Ed, 2008: 583-592.
9.	Morgillo F, Bareschino MA, Bianco R, et al. Primary and acquired resistance to anti-EGFR targeted drugs in cancer therapy [J]. Differentiation, 2007; 75(9): 788-799.
10.	Duesberg P, Li R, Sachs R, et al. Cancer drug resistance: the central role of the karyotype [J]. Drug Resist Updat, 2007; 10(1-2): 51-58.
11.	Ozben T. Mechanisms and strategies to overcome multiple drug resistance in cancer [J]. FEBS Lett, 2006; 580(12): 2903-2909.
12.	Jabr-Milane LS, van Vlerken LE, Yadav S, et al. Multi-functional nanocarriers to overcome tumor drug resistance [J]. Cancer Treat Rev, 2008; 34(7): 592-602.
13.	McConkey DJ, Zhu K. Mechanisms of proteasome inhibitor action and resistance in cancer [J]. Drug Resist Updat, 2008; 11(4-5): 164-179.
14.	Rygaard J, Povlsen CO. Hetero transplantation of a human malignant tumor to nude mice [J]. Act Path Microbiol Scand, 1969; 77(4): 758-760.
15.	刘孟刚, 杨俊涛, 刘宏鸣, 等. GA反义基因对裸鼠胃癌移植瘤细胞凋亡的影响 [J]. 中国普外基础与临床杂志, 2008; 15(1): 4-7.
16.	黄喆, 郭克建, 何三光. 柳氮磺胺吡啶和5-氟尿嘧啶对裸鼠皮下移植瘤胰腺癌细胞BxPC-3凋亡基因的影响 [J]. 中国普外基础与临床杂志, 2008; 15(8): 550-555.
17.	Mickisch GH, Garin A, van Poppel H, et al. Radical nephrectomy plus interferon-alfa-based immunotherapy compared with interferon alfa alone in metastatic renal-cell carcinoma: a randomised trial [J]. Lancet, 2001; 358(9286): 966-970.
18.	Metzinger DS, Taylor DD, Gercel-Taylor C. Induction of p53 and drug resistance following treatment with cisplatin or paclitaxel in ovarian cancer cell lines [J]. Cancer Lett, 2006; 236(2): 302-308.
19.	万继英, 韩庶勇. 肿瘤实验动物模型的建立及应用研究进展 [J]. 医学综述， 2009; 15(19): 2959-2961.
20.	Yoshiba S, Ito D, Nagumo T, et al. Hypoxia induces resistance to 5-fluorouracil in oral cancer cells via G(1) phase cell cycle arrest [J]. Oral Oncol, 2009; 45(2): 109-115.
21.	梁永钜，符立梧，冯海林, 等. KBv200裸鼠移植瘤模型的建立及其耐药特性的探讨 [J] . 中国药理学通报, 2000; 16(6)： 705-707.
22.	Henss H, Fiebig HH, Meinhardt K, et al. Clonal growth of human tumor xenografts. First experiences in drug testing [J]. J Cancer Res Clin Oncol, 1984; 108(2): 233-235.
23.	Kelland LR. Of mice and men: values and liabilities of the athymic nude mouse model in anticancer drug development [J]. Eur J Cancer, 2004; 40(6): 827-836.
24.	Prenen H, Deroose C, Vermaelen P, et al. Establishment of a mouse gastrointestinal stromal tumour model and evaluation of response to imatinib by small animal positron emission tomography [J]. Anticancer Res, 2006; 26(2A): 1247-1252.
25.	Zhang YH, Wu Q, Xiao XY, et al. Silencing MRP4 by small interfering RNA reverses acquired DDP resistance of gastric cancer cell [J]. Cancer Lett, 2010; 291(1): 76-82.
26.	Hawkins LK, Lemoine NR, Kirn D. Oncolytic biotherapy: a novel therapeutic plafform [J]. Lancet Oncol, 2002; 3(1): 17-26.

1. Meyer HJ. The influence of case load and the extent of resection on the quality of treatment outcome in gastric cancer [J]. Eur J Surg Oncol, 2005; 31(6): 595-604.
2. Sivins A, Pedrazzani C, Roviello F, et al. Surgical treatment of gastric cancer in Latvia: results of centralized experience [J]. Eur J Surg Oncol, 2009; 35(5): 481-485.
3. Morabito A, Carillio G, Longo R. Systemic treatment of gastric cancer [J]. Crit Rev Oncol Hematol, 2009; 70(3): 216-234.
4. Cervenak J, Andrikovics H, Ozvegy-Laczka C, et al. The role of the human ABCG2 multidrug transporter and its variants in cancer therapy and toxicology [J]. Cancer Lett, 2006; 234(1): 62-72.
5. Chun JH, Kim HK, Lee JS, et al. Weekly irinotecan in patients with metastatic gastric cancer failing cisplatin-based chemotherapy [J]. Jpn J Clin Oncol, 2004; 34(1): 8-13.
6. 林芸秀, 吕联煌, 林锦娟, 等. 人小细胞肺癌NCI-H446细胞裸鼠异种移植瘤模型的建立 [J]. 实验动物科学与管理, 2004; 21(3): 1-3.
7. 何一, 王崇树, 魏寿江, 等. 人胃癌耐羟基喜树碱细胞株SGC-7901/HCPT 的建立及其生物学性状 [J]. 中国现代医生, 2008; 46(3): 15-17.
8. Branimir IS. Natural and Acquired Resistance to Cancer Therapies [A]. In: John Mendelsohn MD, Peter M. Howley MD, Mark A. Israel MD, et al. The Molecular Basis of Cancer [M]. Third Ed, 2008: 583-592.
9. Morgillo F, Bareschino MA, Bianco R, et al. Primary and acquired resistance to anti-EGFR targeted drugs in cancer therapy [J]. Differentiation, 2007; 75(9): 788-799.
10. Duesberg P, Li R, Sachs R, et al. Cancer drug resistance: the central role of the karyotype [J]. Drug Resist Updat, 2007; 10(1-2): 51-58.
11. Ozben T. Mechanisms and strategies to overcome multiple drug resistance in cancer [J]. FEBS Lett, 2006; 580(12): 2903-2909.
12. Jabr-Milane LS, van Vlerken LE, Yadav S, et al. Multi-functional nanocarriers to overcome tumor drug resistance [J]. Cancer Treat Rev, 2008; 34(7): 592-602.
13. McConkey DJ, Zhu K. Mechanisms of proteasome inhibitor action and resistance in cancer [J]. Drug Resist Updat, 2008; 11(4-5): 164-179.
14. Rygaard J, Povlsen CO. Hetero transplantation of a human malignant tumor to nude mice [J]. Act Path Microbiol Scand, 1969; 77(4): 758-760.
15. 刘孟刚, 杨俊涛, 刘宏鸣, 等. GA反义基因对裸鼠胃癌移植瘤细胞凋亡的影响 [J]. 中国普外基础与临床杂志, 2008; 15(1): 4-7.
16. 黄喆, 郭克建, 何三光. 柳氮磺胺吡啶和5-氟尿嘧啶对裸鼠皮下移植瘤胰腺癌细胞BxPC-3凋亡基因的影响 [J]. 中国普外基础与临床杂志, 2008; 15(8): 550-555.
17. Mickisch GH, Garin A, van Poppel H, et al. Radical nephrectomy plus interferon-alfa-based immunotherapy compared with interferon alfa alone in metastatic renal-cell carcinoma: a randomised trial [J]. Lancet, 2001; 358(9286): 966-970.
18. Metzinger DS, Taylor DD, Gercel-Taylor C. Induction of p53 and drug resistance following treatment with cisplatin or paclitaxel in ovarian cancer cell lines [J]. Cancer Lett, 2006; 236(2): 302-308.
19. 万继英, 韩庶勇. 肿瘤实验动物模型的建立及应用研究进展 [J]. 医学综述， 2009; 15(19): 2959-2961.
20. Yoshiba S, Ito D, Nagumo T, et al. Hypoxia induces resistance to 5-fluorouracil in oral cancer cells via G(1) phase cell cycle arrest [J]. Oral Oncol, 2009; 45(2): 109-115.
21. 梁永钜，符立梧，冯海林, 等. KBv200裸鼠移植瘤模型的建立及其耐药特性的探讨 [J] . 中国药理学通报, 2000; 16(6)： 705-707.
22. Henss H, Fiebig HH, Meinhardt K, et al. Clonal growth of human tumor xenografts. First experiences in drug testing [J]. J Cancer Res Clin Oncol, 1984; 108(2): 233-235.
23. Kelland LR. Of mice and men: values and liabilities of the athymic nude mouse model in anticancer drug development [J]. Eur J Cancer, 2004; 40(6): 827-836.
24. Prenen H, Deroose C, Vermaelen P, et al. Establishment of a mouse gastrointestinal stromal tumour model and evaluation of response to imatinib by small animal positron emission tomography [J]. Anticancer Res, 2006; 26(2A): 1247-1252.
25. Zhang YH, Wu Q, Xiao XY, et al. Silencing MRP4 by small interfering RNA reverses acquired DDP resistance of gastric cancer cell [J]. Cancer Lett, 2010; 291(1): 76-82.
26. Hawkins LK, Lemoine NR, Kirn D. Oncolytic biotherapy: a novel therapeutic plafform [J]. Lancet Oncol, 2002; 3(1): 17-26.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Establishment and Characterization of Human Gastric Cancer Cell Line SGC-7901/HCPT Xenograft in Nude Mice

Abstract Full text Figures/Tables Video References Cited by

Format

Content