Common Reasons for Failure in Orthotopic Liver Transplantation in Rats During Preliminary Experiment and Preventive_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

QIU Changru , WANG Wei , CHENG Wei , SHEN Haoyuan.

Department of Hepatobiliary Surgery, The First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, ChinaCorresponding Author： WANG Wei, E-mail: wwsir8@sohu.com;

Corresponding author：

Keywords：

Rat; Liver transplantation; Failure reason; Prevention; Strategy

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Objective 　To analysis the common reasons for failure in orthotopic liver transplantation during preliminary experiment and propose the preventive.
Methods 　One hundred and twenty cases in preliminary experiment using modified Kamada “two-cuff” method of orthotopic liver transplantation were retrospectively analyzed.
Results 　The causes of failure included： lengthening of anhepatic phase (66 cases)， failed anastomosis of suprahepatic inferior vena cava (61 cases)， failed anastomosis of infrahepatic inferior vena cava (17 cases), failed anastomosis of portal vein (12 cases), unsatisfied anesthesia (8 cases). Succeed in 21 cases (17.50%, 21/120).
Conclusion 　Improve the microsurgical operation techniques, particularly the anastomosis of suprahepatic inferior vena cava, can increase the success rate in orthotopic liver transplantation.

Citation： QIU Changru,WANG Wei,CHENG Wei,SHEN Haoyuan.. Common Reasons for Failure in Orthotopic Liver Transplantation in Rats During Preliminary Experiment and Preventive. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(10): 1035-1038. doi: Copy

1.	Lee S, Charters AC, Chandler JG, et al. A technique for orthotopic liver transplantation in the rat [J]. Transplantation, 1973; 16(6): 664-669．.
2.	Miyata M, Fischer JH, Fuhs M, et al. A simple method for orthotopic liver transplantation in the rat. Cuff technique for three vascular anastomoses [J]. Transplantation, 1980; 30(5): 335-338．.
3.	Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat [J]. Surgery, 1983; 93(1 Pt 1): 64-69.
4.	孙君泓, 吴孟超, 曾琪华. 300次大鼠原位肝移植 [J]. 中华器官移植杂志, 1990; 11(1): 19-21.
5.	董雨亭, 张文海. 大鼠原位肝移植术并发症的预防 [J]. 中华器官移植杂志, 1995; 16(1): 12-14.
6.	黄新立, 张峰, 王学浩, 等. 建立大鼠部分肝移植动脉化模型的实验研究 [J]. 中国普外基础与临床杂志, 2004; 11(3): 224-227.
7.	刘立新, 彭方兴, 张英男, 等. 改良法大鼠原位肝移植死亡因素分析及对策 [J]. 中国普外基础与临床杂志, 2005; 12(2): 120-122.
8.	彭方兴, 郑本波, 刘立新, 等. 大鼠原位肝移植动物模型制作要点 [J]. 中国普外基础与临床杂志, 2005; 12(2): 117-120.
9.	Kashfi A, Mehrabi A, Pahlavan PS, et al. A review of various techniques of orthotopic liver transplantation in the rat [J]. Transplant Proc, 2005; 37(1): 185-188.
10.	朱金海, 陈燕凌, 唐南洪, 等. 大鼠改良式原位肝移植手术技巧探讨 [J]. 中国修复重建外科杂志, 2008; 22(11): 1384-1388.
11.	Jiang Y, Qiu YD, Gu XP, et al. Rat liver transplantation without preservation of “phrenic ring” using double cuff method [J]. World J Gastroenterol, 2004; 10(10): 1526-1527.
12.	Matevossiana E, Dolla D, Hüsera N, et al. Liver transplantation in the rat: Single-center experience wth technique, long-term survival, and functional and histologic findings [J]. Transplant Proc, 2009; 41(6): 2631-2636.
13.	Hübscher SG. Transplantation pathology [J]. Semin Liver Dis, 2009; 29(1): 74-90.
14.	彭勇, 龚建平, 刘长安, 等. 大鼠原位肝移植模型制作过程中麻醉方法的选择 [J]. 中国普通外科杂志, 2003; 12(9): 673-676.

1. Lee S, Charters AC, Chandler JG, et al. A technique for orthotopic liver transplantation in the rat [J]. Transplantation, 1973; 16(6): 664-669．.
2. 　Miyata M, Fischer JH, Fuhs M, et al. A simple method for orthotopic liver transplantation in the rat. Cuff technique for three vascular anastomoses [J]. Transplantation, 1980; 30(5): 335-338．.
3. 　Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat [J]. Surgery, 1983; 93(1 Pt 1): 64-69.
4. 　孙君泓, 吴孟超, 曾琪华. 300次大鼠原位肝移植 [J]. 中华器官移植杂志, 1990; 11(1): 19-21.
5. 　董雨亭, 张文海. 大鼠原位肝移植术并发症的预防 [J]. 中华器官移植杂志, 1995; 16(1): 12-14.
6. 　黄新立, 张峰, 王学浩, 等. 建立大鼠部分肝移植动脉化模型的实验研究 [J]. 中国普外基础与临床杂志, 2004; 11(3): 224-227.
7. 　刘立新, 彭方兴, 张英男, 等. 改良法大鼠原位肝移植死亡因素分析及对策 [J]. 中国普外基础与临床杂志, 2005; 12(2): 120-122.
8. 　彭方兴, 郑本波, 刘立新, 等. 大鼠原位肝移植动物模型制作要点 [J]. 中国普外基础与临床杂志, 2005; 12(2): 117-120.
9. 　Kashfi A, Mehrabi A, Pahlavan PS, et al. A review of various techniques of orthotopic liver transplantation in the rat [J]. Transplant Proc, 2005; 37(1): 185-188.
10. 　朱金海, 陈燕凌, 唐南洪, 等. 大鼠改良式原位肝移植手术技巧探讨 [J]. 中国修复重建外科杂志, 2008; 22(11): 1384-1388.
11. 　Jiang Y, Qiu YD, Gu XP, et al. Rat liver transplantation without preservation of “phrenic ring” using double cuff method [J]. World J Gastroenterol, 2004; 10(10): 1526-1527.
12. 　Matevossiana E, Dolla D, Hüsera N, et al. Liver transplantation in the rat: Single-center experience wth technique, long-term survival, and functional and histologic findings [J]. Transplant Proc, 2009; 41(6): 2631-2636.
13. 　Hübscher SG. Transplantation pathology [J]. Semin Liver Dis, 2009; 29(1): 74-90.
14. 　彭勇, 龚建平, 刘长安, 等. 大鼠原位肝移植模型制作过程中麻醉方法的选择 [J]. 中国普通外科杂志, 2003; 12(9): 673-676.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Common Reasons for Failure in Orthotopic Liver Transplantation in Rats During Preliminary Experiment and Preventive

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