Effect and Evaluation ofStem Cell for Blood Flow Remodeling inCritical Limb Ischemia_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANGYong ¹ , LU Ping ¹ , HE Xiaoming ¹ , YANG Guokai ¹ , LUO Kaiyuan ¹ , DUAN Zhiquan ² , SHI De. ³

1.Department of Vascular Surgery,The Fourth Affiliated Hospital of Kunming Medical College Kunming 650021,China;;
2.Department of Vascular Surgery,The First Affiliated Hospital of China Medical University,Shenyang 110001,China;;
3.Department of Vascular Surgery,The First Affiliated Hospital of Chongqing Medical University,Chongqing 400010,ChinaCorresponding Author: YANG Yong, E-mail:yncvs@yahoo.com.cn;

Corresponding author：

Keywords：

Stem cell; Limb; Ischemia; Effect; Index

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Objective To explore the effect and evaluation criterion of the transplantation of autologous peripheral blood stem cells(PBSC)for blood flow remodeling in the critical limb ischemia (CLI).
MethodsThirty six patients with 39 limbs suffered from CLI at Yunnan Provincial Center of Vascular Surgery and Department of Vascular Surgery of The First Affiliated Hospital of China Medical University from March 2003 to January 2007 were included in this study.These patients were divided into two groups. One groupconsisted of 20 cases in all 22 limbs used the transplantation of autologous PBSC,and another group included 16 cases in all 17 limbs were not use this technique.Multifunction monitoring device,dopplor ultrasound monitoring device,per cutem oxygen partial pressure monitoring device and digital subtraction angiography were used to measure effect degree of limbs regional blood flow from cutaneous covering,blood vessel and blood on the preoperative and postoperative days and the follow-up time was six months.
ResultsThe effect indexes of limbs regional blood flow of the case by transplantation of autologous PBSC was as follows:skin temperature index(STI)was(1.5±0.3) ℃， per cutem oxygen partial pressure monitoring device(TcPO2)was(36.6±9.3)mm Hg,ankle-brachium index(ABI)was0.7±0.1,photoplethysmograpy index(PPGI) was0.8±0.1,saturation of blood oxygen(SaO2)was(78.3±15.9)%,digital subtraction angiography score was1.5±0.3,the rate of limbs salvage was 82%,the distance of intermittent claudication was(150.3±41.1)meters,and the change of index was consistent with ameliorative tendency of symptom(0.415＜r＜0.592, P＜0.05).
ConclusionThe transplantation of autologous PBSC can promote blood flowremodeling in limbs
ischemia,and the effect indexes of limbs regional blood flow can objectively reflect the degree of blood flow remodeling.

Citation： YANGYong,LU Ping,HE Xiaoming,YANG Guokai,LUO Kaiyuan,DUAN Zhiquan,SHI De.. Effect and Evaluation ofStem Cell for Blood Flow Remodeling inCritical Limb Ischemia. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(2): 115-118. doi: Copy

1.	段志泉，张强主编.实用血管外科学 [M].第1版.沈阳:辽宁科学技术出版社, 1999：75～76.
2.	段志泉，张强主编. 实用血管外科学 [M]. 第1版.沈阳: 辽宁科学技术出版社,1999： 406～407.
3.	Oyibo SO,Jude EB,Tarawneh I,et al. A comparison of two diabetic foot ulcer classification systems:the Wagner and the University of Texas wound classification systems[Ｊ].Diabetes Care,2001;24(1)：84.
4.	汪忠稿，张建，谷涌泉主编.血栓闭塞性脉管炎 [M]. 第1版.北京: 人民军医出版社， 2004：266～271.
5.	王玉琦，史振宇.我国血管外科的现状与展望 [Ｊ]. 中国普外基础与临床杂志， 2008;15(6)：387.
6.	梁越洋，吴诚义.造血干细胞微环境中的相互调节 [Ｊ].中国普外基础与临床杂志,2008;15(3)：171.
7.	寇兰，张韵. 自体外周血干细胞移植治疗下肢缺血的临床进展 [Ｊ].肿瘤预防与治疗,2008; 21(1)：104.
8.	杨镛，罗开元，段志泉，等.自体外周血干细胞移植重建缺血肢体血管功能的策略与问题 [Ｊ]. 云南医药,2005;26 (3B)∶7.
9.	黄平平，李尚珠，韩明哲，等.自体外周血干细胞移植治疗下肢动脉硬化性闭塞症 [Ｊ].中华血液学杂志， 2003; 24(6)： 308.
10.	谷涌泉，张健，齐立行，等.自体骨髓干细胞移植治疗慢性下肢缺血94例不同病变分期患者的效果比较 [Ｊ]. 中国康复临床， 2005; 9(38)： 7.
11.	郭连瑞，谷涌泉，张健，等.不同途径移植骨髓单个核细胞治疗大鼠后肢缺血 [Ｊ].中国临床康复,2005； 9(10)： 57.
12.	Huang PP, Li SZ,Han MZ, et al. Autologous transplantation of granulocyte colony-stimulating factor mobilized peripheral blood mononuclear cells improves critical limb ischemia in diabetes[Ｊ]. Diabetes Care,2005; 28(9)： 2155.
13.	谷涌泉，郭连瑞，张建，等. 自体骨髓干细胞移植改善下肢严重缺血致运动功能障碍: 15例报告 [Ｊ]. 中国临床康复， 2004;8(20)：3917.
14.	吴雁翔，杨晓凤，王红梅，等. 干细胞治疗下肢缺血性疾病疗效观察 [Ｊ]. 中国修复重建外科杂志， 2005； 19(12)： 1019.
15.	缪健航，师天雄，胡锡祥，等. 自体外周血干细胞移植治疗下肢动脉缺血性疾病的临床应用 [Ｊ].中国普外基础与临床杂志， 2008;15(2)： 106.
16.	张健，段志泉. 下肢动脉硬化闭塞症的治疗进展 [Ｊ].中国普外基础与临床杂志， 2004;11(4)： 288.

1. 段志泉，张强主编.实用血管外科学 [M].第1版.沈阳:辽宁科学技术出版社, 1999：75～76.
2. 段志泉，张强主编. 实用血管外科学 [M]. 第1版.沈阳: 辽宁科学技术出版社,1999： 406～407.
3. Oyibo SO,Jude EB,Tarawneh I,et al. A comparison of two diabetic foot ulcer classification systems:the Wagner and the University of Texas wound classification systems[Ｊ].Diabetes Care,2001;24(1)：84.
4. 汪忠稿，张建，谷涌泉主编.血栓闭塞性脉管炎 [M]. 第1版.北京: 人民军医出版社， 2004：266～271.
5. 王玉琦，史振宇.我国血管外科的现状与展望 [Ｊ]. 中国普外基础与临床杂志， 2008;15(6)：387.
6. 梁越洋，吴诚义.造血干细胞微环境中的相互调节 [Ｊ].中国普外基础与临床杂志,2008;15(3)：171.
7. 寇兰，张韵. 自体外周血干细胞移植治疗下肢缺血的临床进展 [Ｊ].肿瘤预防与治疗,2008; 21(1)：104.
8. 杨镛，罗开元，段志泉，等.自体外周血干细胞移植重建缺血肢体血管功能的策略与问题 [Ｊ]. 云南医药,2005;26 (3B)∶7.
9. 黄平平，李尚珠，韩明哲，等.自体外周血干细胞移植治疗下肢动脉硬化性闭塞症 [Ｊ].中华血液学杂志， 2003; 24(6)： 308.
10. 谷涌泉，张健，齐立行，等.自体骨髓干细胞移植治疗慢性下肢缺血94例不同病变分期患者的效果比较 [Ｊ]. 中国康复临床， 2005; 9(38)： 7.
11. 郭连瑞，谷涌泉，张健，等.不同途径移植骨髓单个核细胞治疗大鼠后肢缺血 [Ｊ].中国临床康复,2005； 9(10)： 57.
12. Huang PP, Li SZ,Han MZ, et al. Autologous transplantation of granulocyte colony-stimulating factor mobilized peripheral blood mononuclear cells improves critical limb ischemia in diabetes[Ｊ]. Diabetes Care,2005; 28(9)： 2155.
13. 谷涌泉，郭连瑞，张建，等. 自体骨髓干细胞移植改善下肢严重缺血致运动功能障碍: 15例报告 [Ｊ]. 中国临床康复， 2004;8(20)：3917.
14. 吴雁翔，杨晓凤，王红梅，等. 干细胞治疗下肢缺血性疾病疗效观察 [Ｊ]. 中国修复重建外科杂志， 2005； 19(12)： 1019.
15. 缪健航，师天雄，胡锡祥，等. 自体外周血干细胞移植治疗下肢动脉缺血性疾病的临床应用 [Ｊ].中国普外基础与临床杂志， 2008;15(2)： 106.
16. 张健，段志泉. 下肢动脉硬化闭塞症的治疗进展 [Ｊ].中国普外基础与临床杂志， 2004;11(4)： 288.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect and Evaluation ofStem Cell for Blood Flow Remodeling inCritical Limb Ischemia

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