Objective To establish and modify a rat model of arterialized small-for-size orthotopic liver transplantation and investigate the histopathologic changes of the grafts after transplantation.
Methods Modified two-cuff technique was applied to establish a rat model of 40% small-for-size orthotopic liver transplantation with a modified microvascular “sleeve” anastomosis between the celiac trunk of donors and the stump of right kidney artery of recipients. Seven days survival rate was observed, main indices of liver function, histopathologic changes of the grafts were detected on the 1st, 2nd, 4th and 7th day after transplantation, respectively.
Results The successful rate of operation was 93.3%. Seven days survival rate was 60.0%. The mean time of nonhepatic time was (12.0±2.5) min. Alanine aminotransferase (ALT) and total bilirubin (TB) began to elevate on the first day and peaked on the second day after operation. Histological findings indicated that hepatic sinusoidal and central vein dilation, monocytes infiltration in partial area were found on the 1st day after operation, more diploid and polyploid hepatocytes could be observed on the 4th day after operation.
Conclusion The model is easily available and highly reproducible, and the stability of the model is improved by modifying the technique. The histological changes of the grafts are mainly caused by ischemia-reperfusion injury.
Citation：
XU Guiyun,JIANG Yi,CHEN Haiyan,PAN Fan. Establishment of Arterialized Small-for-Size Liver Transplantation Model and Investigation of Histopathologic Changes of Grafts in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(3): 180-183. doi:
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Heaton N. Small-for-size liver syndrome after auxiliary and split liver transplantation: donor selection [Ｊ]. Liver Transpl, 2003; 9(9):S26.
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Lo CM, Fan ST, Liu CL, et al. Minimum graft size for successful living donor liver transplantation [Ｊ]. Transplantation, 1999; 68(8):1112.
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Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ]. Transplantation, 1979; 28(1):47.
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徐贵云， 江艺， 蔡秋程， 等. 改良套入缝合重建肝动脉血供的大鼠肝移植模型建立 [Ｊ]. 肝胆外科杂志， 2008; 16(1):63.
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Knoop M, Bachmann S, Keck H, et al. Experience with cuff rearterialization in 600 orthotopic liver grafts in the rat [Ｊ]. Am J Surg, 1994; 167(3):360.
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Li J, Dahmen U, Dirsch O, et al. Modified sleeve anastomosis for reconstruction of the hepatic artery in rat liver transplantation [Ｊ]. Microsurgery, 2002; 22(2):62.
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黄新立, 张峰, 王学浩, 等. 建立大鼠部分肝移植动脉化模型的实验研究 [Ｊ]. 中国普外基础与临床杂志, 2004; 11(5):224.
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梁廷波， 郑树森， 徐骁， 等. 两种不同方法的大鼠小体积肝移植模型建立的比较 [Ｊ]. 中华肝胆外科杂志， 2004; 10(5):320.
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钱建民， 汤黎明， 胡建平， 等. 大鼠肝移植小移植物模型的研究 [Ｊ]. 中华实验外科杂志， 2003; 20(3):275.
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Xu HS, Pruett TL, Jones RS. Study of donor-recipient liver size match for transplantation [Ｊ]. Ann Surg, 1994; 219(1):46.
|
| 11. |
Man K, Lo CM, Ng IO, et al. Liver transplantation in rats using small-for-size grafts: a study of hemodynamic and morphological changes [Ｊ]. Arch Surg, 2001; 136(3):280.
|
- 1. Heaton N. Small-for-size liver syndrome after auxiliary and split liver transplantation: donor selection [Ｊ]. Liver Transpl, 2003; 9(9):S26.
- 2. Lo CM, Fan ST, Liu CL, et al. Minimum graft size for successful living donor liver transplantation [Ｊ]. Transplantation, 1999; 68(8):1112.
- 3. Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ]. Transplantation, 1979; 28(1):47.
- 4. 徐贵云， 江艺， 蔡秋程， 等. 改良套入缝合重建肝动脉血供的大鼠肝移植模型建立 [Ｊ]. 肝胆外科杂志， 2008; 16(1):63.
- 5. Knoop M, Bachmann S, Keck H, et al. Experience with cuff rearterialization in 600 orthotopic liver grafts in the rat [Ｊ]. Am J Surg, 1994; 167(3):360.
- 6. Li J, Dahmen U, Dirsch O, et al. Modified sleeve anastomosis for reconstruction of the hepatic artery in rat liver transplantation [Ｊ]. Microsurgery, 2002; 22(2):62.
- 7. 黄新立, 张峰, 王学浩, 等. 建立大鼠部分肝移植动脉化模型的实验研究 [Ｊ]. 中国普外基础与临床杂志, 2004; 11(5):224.
- 8. 梁廷波， 郑树森， 徐骁， 等. 两种不同方法的大鼠小体积肝移植模型建立的比较 [Ｊ]. 中华肝胆外科杂志， 2004; 10(5):320.
- 9. 钱建民， 汤黎明， 胡建平， 等. 大鼠肝移植小移植物模型的研究 [Ｊ]. 中华实验外科杂志， 2003; 20(3):275.
- 10. Xu HS, Pruett TL, Jones RS. Study of donor-recipient liver size match for transplantation [Ｊ]. Ann Surg, 1994; 219(1):46.
- 11. Man K, Lo CM, Ng IO, et al. Liver transplantation in rats using small-for-size grafts: a study of hemodynamic and morphological changes [Ｊ]. Arch Surg, 2001; 136(3):280.
