Relationship Between Expressions of MCP-1 and VEGF in Gastric Cancer and Helicobacter Pylori Infection_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Lei , SUN Xuejun , WANG Wei , ZHENG Jianbao , SUN Fenglei , LU Shaoying.

Department of General Surgery, The First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710061, China;

Corresponding author：

Keywords：

Monocyte chemoattractant protein-1; Vascular endothelial growth factor; Helicobacter pylori; Gastric cancer; Immunohistochemistry

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Objective To investigate the expressions of monocyte chemoattractant protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) in gastric cancer tissues and normal gastirc mucosa tissues and the situation of helicobacter pylori (HP) infection, and detect their relationships and clinicopathologic significances. Methods Expressions of MCP-1 and VEGF were detected by immunohistochemistry in gastric cancer tissues and normal gastric mucosa tissues (5-10 cm from the mass), and HP was detected in specimen from gastric antrum by Giemsa dyeing method. Results MCP-1 and VEGF expressions in gastric cancer tissues were significantly higher than those in normal gastric mucosa tissues (P＜0.05), but there was no difference in HP positive and negative tissues included the cancer and the normal tissues （P＞0.05）. The expressions of MCP-1 and VEGF in carcinoma with tumordiameter ＞5 cm, poorly differentiated， lymph node metastasis， distant metastasis and Ⅲ+Ⅳ stage of TNM were significantly higher than those with tumor diameter ≤5 cm, well and moderately differentiated， non-lymph node metastasis， non-distant metastasis and Ⅰ+Ⅱ stage of TNM (P＜0.05). Conclusion The high expressions of MCP-1 and VEGF in gastric cancer may relate to tumor angiogenesis and metastasis, but HP infection may be irrelevant.

Citation： YANG Lei,SUN Xuejun,WANG Wei,ZHENG Jianbao,SUN Fenglei,LU Shaoying.. Relationship Between Expressions of MCP-1 and VEGF in Gastric Cancer and Helicobacter Pylori Infection. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(5): 375-379. doi: Copy

1.	Houghton J, Wang TC. Helicobacter pylori and gastric cancer: a new paradigm for inflammation-associated epithelial cancers [Ｊ]. Gastroenterology, 2005; 128(6): 1567-1578.
2.	Singh S, Sadanandam A, Singh RK. Chemokines in tumor angiogenesis and metastasis [Ｊ]. Cancer Metastasis Rev, 2007; 26(3-4): 453-467.
3.	Koga M, Kai H, Egami K, et al. Mutant MCP-1 therapy inhibits tumor angiogenesis and growth of malignant melanoma in mice [Ｊ]. Biochem Biophys Res Commun, 2008; 365(2): 279-284.
4.	Yoo CH, Noh SH, Kim YI, et al. Comparison of prognostic significance of nodal staging between old (4th edition) and new (5th edition) UICC TNM classification for gastric carcinoma.International Union Against Cancer [Ｊ]. World J Surg, 1999; 23(5): 492-498.
5.	Laine L, Lewin DN, Naritoku W, et al. Prospective comparison of H&E, Giemsa, and Genta stains for the diagnosis of Helicobacter pylori [Ｊ]. Gastrointest Endosc, 1997; 45(6): 463-467.
6.	谭明, 戴春, 丁永斌, 等. syndecan-1蛋白在胃癌中的表达及其意义 [Ｊ]. 中国普外基础与临床杂志, 2008; 15(12): 910-913.
7.	姚宏亮, 杨竹林, 李永国, 等. 胃癌及癌旁组织中 SKP2 和p27 蛋白表达及相互关系研究 [Ｊ]. 中国普外基础与临床杂志, 2007; 14(3): 308-311.
8.	Henkel JS, Beers DR, Siklós L, et al. The chemokine MCP-1 and the dendritic and myeloid cells it attracts are increased in the mSOD1 mouse model of ALS [Ｊ]. Mol Cell Neurosci, 2006; 31(3): 427-437.
9.	Chipitsyna G, Gong Q, Gray CF, et al. Induction of monocyte chemoattractant protein-1 expression by angiotensin Ⅱ in the pancreatic islets and beta-cells [Ｊ]. Endocrinology, 2007; 148(5): 2198-2208.
10.	Kim HL, Lee DS, Yang SH, et al. The polymorphism of monocyte chemoattractant protein-1 is associated with the renal disease of SLE [Ｊ]. Am J Kidney Dis, 2002; 40(6): 1146-1152.
11.	Tanaka E, Shimokawa H, Kamiuneten H, et al. Disparity of MCP-1 mRNA and protein expressions between the carotid artery and the aorta in WHHL rabbits: one aspect involved in the regional difference in atherosclerosis [Ｊ]. Arterioscler Thromb Vasc Biol, 2003; 23(2): 244-250.
12.	Conti I, Rollins BJ. CCL2 (monocyte chemoattractant protein-1) and cancer [J]. Semin Cancer Biol， 2004; 14(3): 149-154.
13.	Valkovic＇ T, Fuckar D, Stifter S, et al. Macrophage level is not affected by monocyte chemotactic protein-1 in invasive ductal breast carcinoma [Ｊ]. J Cancer Res Clin Oncol, 2005; 131(7): 453-458.
14.	Futagami S, Tatsuguchi A, Hiratsuka T, et al. Monocyte chemoattractant protein 1 and CD40 ligation have a synergistic effect on vascular endothelial growth factor production through cyclooxygenase 2 upregulation in gastric cancer [Ｊ]. J Gastroenterol, 2008; 43(3): 216-224.
15.	Koga M, Kai H, Egami K, et al. Mutant MCP-1 therapy inhibits tumor angiogenesis and growth of malignant melanoma in mice [Ｊ]. Biochem Biophys Res Commun, 2008; 365(2): 279-284.
16.	Marcus B, Arenberg D, Lee J, et al. Prognostic factors in oral cavity and oropharyngeal squamous cell carcinoma [Ｊ]. Cancer, 2004; 101(12): 2779-2787.
17.	佟书娟, 刘亚平, 伊清云. 幽门螺杆菌感染与胃癌中VEGF的表达及肿瘤血管形成的关系 [Ｊ]. 实用肿瘤学杂志, 2005; 19(3): 161-163.

1. Houghton J, Wang TC. Helicobacter pylori and gastric cancer: a new paradigm for inflammation-associated epithelial cancers [Ｊ]. Gastroenterology, 2005; 128(6): 1567-1578.
2. Singh S, Sadanandam A, Singh RK. Chemokines in tumor angiogenesis and metastasis [Ｊ]. Cancer Metastasis Rev, 2007; 26(3-4): 453-467.
3. Koga M, Kai H, Egami K, et al. Mutant MCP-1 therapy inhibits tumor angiogenesis and growth of malignant melanoma in mice [Ｊ]. Biochem Biophys Res Commun, 2008; 365(2): 279-284.
4. Yoo CH, Noh SH, Kim YI, et al. Comparison of prognostic significance of nodal staging between old (4th edition) and new (5th edition) UICC TNM classification for gastric carcinoma.International Union Against Cancer [Ｊ]. World J Surg, 1999; 23(5): 492-498.
5. Laine L, Lewin DN, Naritoku W, et al. Prospective comparison of H&E, Giemsa, and Genta stains for the diagnosis of Helicobacter pylori [Ｊ]. Gastrointest Endosc, 1997; 45(6): 463-467.
6. 谭明, 戴春, 丁永斌, 等. syndecan-1蛋白在胃癌中的表达及其意义 [Ｊ]. 中国普外基础与临床杂志, 2008; 15(12): 910-913.
7. 姚宏亮, 杨竹林, 李永国, 等. 胃癌及癌旁组织中 SKP2 和p27 蛋白表达及相互关系研究 [Ｊ]. 中国普外基础与临床杂志, 2007; 14(3): 308-311.
8. Henkel JS, Beers DR, Siklós L, et al. The chemokine MCP-1 and the dendritic and myeloid cells it attracts are increased in the mSOD1 mouse model of ALS [Ｊ]. Mol Cell Neurosci, 2006; 31(3): 427-437.
9. Chipitsyna G, Gong Q, Gray CF, et al. Induction of monocyte chemoattractant protein-1 expression by angiotensin Ⅱ in the pancreatic islets and beta-cells [Ｊ]. Endocrinology, 2007; 148(5): 2198-2208.
10. Kim HL, Lee DS, Yang SH, et al. The polymorphism of monocyte chemoattractant protein-1 is associated with the renal disease of SLE [Ｊ]. Am J Kidney Dis, 2002; 40(6): 1146-1152.
11. Tanaka E, Shimokawa H, Kamiuneten H, et al. Disparity of MCP-1 mRNA and protein expressions between the carotid artery and the aorta in WHHL rabbits: one aspect involved in the regional difference in atherosclerosis [Ｊ]. Arterioscler Thromb Vasc Biol, 2003; 23(2): 244-250.
12. Conti I, Rollins BJ. CCL2 (monocyte chemoattractant protein-1) and cancer [J]. Semin Cancer Biol， 2004; 14(3): 149-154.
13. Valkovic＇ T, Fuckar D, Stifter S, et al. Macrophage level is not affected by monocyte chemotactic protein-1 in invasive ductal breast carcinoma [Ｊ]. J Cancer Res Clin Oncol, 2005; 131(7): 453-458.
14. Futagami S, Tatsuguchi A, Hiratsuka T, et al. Monocyte chemoattractant protein 1 and CD40 ligation have a synergistic effect on vascular endothelial growth factor production through cyclooxygenase 2 upregulation in gastric cancer [Ｊ]. J Gastroenterol, 2008; 43(3): 216-224.
15. Koga M, Kai H, Egami K, et al. Mutant MCP-1 therapy inhibits tumor angiogenesis and growth of malignant melanoma in mice [Ｊ]. Biochem Biophys Res Commun, 2008; 365(2): 279-284.
16. Marcus B, Arenberg D, Lee J, et al. Prognostic factors in oral cavity and oropharyngeal squamous cell carcinoma [Ｊ]. Cancer, 2004; 101(12): 2779-2787.
17. 佟书娟, 刘亚平, 伊清云. 幽门螺杆菌感染与胃癌中VEGF的表达及肿瘤血管形成的关系 [Ｊ]. 实用肿瘤学杂志, 2005; 19(3): 161-163.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Relationship Between Expressions of MCP-1 and VEGF in Gastric Cancer and Helicobacter Pylori Infection

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