Effects of Fluorouracil Against The Growth and Metastasis of Tumor Cells in Carbon Dioxide Pneumoperitoneum_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SUN Tiewei , LI Qiang , WANG Feng

The Seventh Department of Genaral Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China;

Corresponding author：

Keywords：

Fluorouracil; Carbon dioxide pneumoperitoneum; Tumor; Growth; Metastasis

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Objective To explore the effects of intraperitoneal chemotherapy with fluorouracil (FU) on the growth and metastasis of tumor cells in carbon dioxide (CO2) pneumoperitoneum.
Methods Fifty male H-22 mice of clean grade were selected and randomly assigned into 5 groups in each group with 10: simple implantation group, pneumoperitoneum group, pneumoperitoneum and NS group, pneumoperitoneum and low concentration (5.0 g/L) of FU group and pneumoperitoneum and high concentration (10.0 g/L) of FU group. All mice were executed after 11 days to observe the weight and the implantation of tumor in abdominal wall. Then the expressions of proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) were detected by immunohistochemistry.
Results The tumor weight was significantly higher in pneumoperitoneum and high concentration of FU group compared with other groups except pneumoperitoneum and low concentration of FU group (P＜0.05, P＜0.01 ). The inhibition rate of tumor was 64.5% in pneumoperitoneum and high concentration of FU group. The diameter of portsite implantation nodus was significantly bigger in pneumoperitoneum and NS group compared with pneumoperitoneum and low concentration of FU group and pneumoperitoneum and high concentration of FU group (P＜0.01). The expressions of PCNA and VEGF of ascites and portsite implantation nodus were significantly different in every group, respectively (P＜0.05, P＜0.01).
Conclusion There is inhibitive effect of intraperitoneal chemotherapy with high concentration of FU on the growth and metastasis of S-180 tumor cells in CO2 pneumoperitoneum, which may be associated with downregulation of PCNA and VEGF expressions.

Citation： SUN Tiewei,LI Qiang,WANG Feng. Effects of Fluorouracil Against The Growth and Metastasis of Tumor Cells in Carbon Dioxide Pneumoperitoneum. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(7): 545-549. doi: Copy

1.	Volz J, Kster S, Schaeff B, et al. Laparoscopic surgery: the effects of insufflation gas on tumorinduced lethality in nude mice [Ｊ]. Am J Obstet Gynecol, 1998; 178(4): 793795.
2.	王广义，孟宪瑛，顾建华，等. CO2气腹对肿瘤细胞种植与生长的影响 [Ｊ]. 中国普外基础与临床杂志, 2004; 11(2): 133135.
3.	Cavina E, Goletti O, Molea N, et al. Trocar site tumor recurrences. May pneumoperitoneum be responsible? [Ｊ]. Surg Endosc, 1998; 12(11): 12941296.
4.	Ridgway PF, Smith A, Ziprin P, et al. Pneumoperitoneum augmented tumor invasiveness is abolished by matrix metalloproteinase blockade [Ｊ]. Surg Endosc, 2002; 16(3): 533536.
5.	韦艾凌，唐健. 癌痛消胶囊调节小鼠荷H22移植性肝癌细胞VEGF表达的实验研究 [Ｊ]. 广西中医药, 2004; 27(4): 4749.
6.	Texler ML, King G, Hewett PJ. Tumor cell movement during heating and humidification of insufflating CO2: an in vitro model [Ｊ]. Aust N Z J Surg, 1998; 68(10): 740742.
7.	Wittich P, Steyerberg EW, Simons SH, et al. Intraperitoneal tumor growth is influenced by pressure of carbon dioxide pneumoperitoneum[Ｊ]. Surg Endosc, 2000; 14(9): 817819.
8.	王灏, 郑民华, 张浩波, 等. CO2气腹对人肿瘤细胞腹腔镜戳口种植的影响 [Ｊ]. 外科理论与实践, 1999; 4(3): 141143.
9.	Kuntz C, Wunsch A, Bdeker C, et al. Effect of pressure and gas type on intraabdominal, subcutaneous, and blood pH in laparoscopy[Ｊ]. Surg Endosc, 2000; 14(4): 367371.
10.	Lee SW, Whelan RL, Southall JC, et al. Abdominal wound tumor recurrence after open and laparoscopicassisted splenectomy in a murine model [Ｊ]. Dis Colon Rectum, 1998; 41(7): 824831.
11.	Neuhaus SJ, Watson DI, Ellis T, et al. Influence of cytotoxic agents on intraperitoneal tumor implantation after laparoscopy[Ｊ]. Dis Colon Rectum, 1999; 42(1): 1015.
12.	Krishna TS, Kong XP, Gary S, et al. Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA [Ｊ]. Cell, 1994; 79(7): 12331243.
13.	Waga S, Hannon GJ, Beach D, et al. The p21 inhibitor of cyclindependent kinases controls DNA replication by interaction with PCNA [Ｊ]. Nature, 1994; 369(6481): 574578.
14.	孙晓敏, 董卫国, 余保平, 等. 检测恶性腹水中VEGF、CD44v6和MMP2、MMP9的临床意义 [Ｊ]. 癌症, 2004; 23(1): 8589.
15.	Mori A, Arii S, Furutani M, et al. Soluble Flt1 gene therapy for peritoneal metastases using HVJcationic liposomes [Ｊ]. Gene Ther, 2000; 7(12): 10271033.

1. Volz J, Kster S, Schaeff B, et al. Laparoscopic surgery: the effects of insufflation gas on tumorinduced lethality in nude mice [Ｊ]. Am J Obstet Gynecol, 1998; 178(4): 793795.
2. 王广义，孟宪瑛，顾建华，等. CO2气腹对肿瘤细胞种植与生长的影响 [Ｊ]. 中国普外基础与临床杂志, 2004; 11(2): 133135.
3. Cavina E, Goletti O, Molea N, et al. Trocar site tumor recurrences. May pneumoperitoneum be responsible? [Ｊ]. Surg Endosc, 1998; 12(11): 12941296.
4. Ridgway PF, Smith A, Ziprin P, et al. Pneumoperitoneum augmented tumor invasiveness is abolished by matrix metalloproteinase blockade [Ｊ]. Surg Endosc, 2002; 16(3): 533536.
5. 韦艾凌，唐健. 癌痛消胶囊调节小鼠荷H22移植性肝癌细胞VEGF表达的实验研究 [Ｊ]. 广西中医药, 2004; 27(4): 4749.
6. Texler ML, King G, Hewett PJ. Tumor cell movement during heating and humidification of insufflating CO2: an in vitro model [Ｊ]. Aust N Z J Surg, 1998; 68(10): 740742.
7. Wittich P, Steyerberg EW, Simons SH, et al. Intraperitoneal tumor growth is influenced by pressure of carbon dioxide pneumoperitoneum[Ｊ]. Surg Endosc, 2000; 14(9): 817819.
8. 王灏, 郑民华, 张浩波, 等. CO2气腹对人肿瘤细胞腹腔镜戳口种植的影响 [Ｊ]. 外科理论与实践, 1999; 4(3): 141143.
9. Kuntz C, Wunsch A, Bdeker C, et al. Effect of pressure and gas type on intraabdominal, subcutaneous, and blood pH in laparoscopy[Ｊ]. Surg Endosc, 2000; 14(4): 367371.
10. Lee SW, Whelan RL, Southall JC, et al. Abdominal wound tumor recurrence after open and laparoscopicassisted splenectomy in a murine model [Ｊ]. Dis Colon Rectum, 1998; 41(7): 824831.
11. Neuhaus SJ, Watson DI, Ellis T, et al. Influence of cytotoxic agents on intraperitoneal tumor implantation after laparoscopy[Ｊ]. Dis Colon Rectum, 1999; 42(1): 1015.
12. Krishna TS, Kong XP, Gary S, et al. Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA [Ｊ]. Cell, 1994; 79(7): 12331243.
13. Waga S, Hannon GJ, Beach D, et al. The p21 inhibitor of cyclindependent kinases controls DNA replication by interaction with PCNA [Ｊ]. Nature, 1994; 369(6481): 574578.
14. 孙晓敏, 董卫国, 余保平, 等. 检测恶性腹水中VEGF、CD44v6和MMP2、MMP9的临床意义 [Ｊ]. 癌症, 2004; 23(1): 8589.
15. Mori A, Arii S, Furutani M, et al. Soluble Flt1 gene therapy for peritoneal metastases using HVJcationic liposomes [Ｊ]. Gene Ther, 2000; 7(12): 10271033.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Fluorouracil Against The Growth and Metastasis of Tumor Cells in Carbon Dioxide Pneumoperitoneum

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