Effects of Enteral Immunonutrition and Econutrition on Intestinal Mucosa Barrier Function in Wounded Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YU Lijian ¹ , XU Liang ² , YAO Hui ² , WEI Renzhi ² , QI Xiaolong ²

1.Department of Anal-Colorectal Surgery of The People’s Hospital of Luzhou City, Luzhou 646000， China;;
2.Department of Gastrointestinal Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China;

Corresponding author：

Keywords：

Enteral nutrition; Immunonutrition; Econutrition; Animal experiment; Wistar rat

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To study effects of enteral immunonutrition and econutrition on intestinal mucosa barrier function in wounded rats. Methods Forty Wistar rats were randomly divided into four groups, with ten rats in each group 〔ie.control group, enteral nutrition (EN) group, enteral immunonutrition (EIN) group and enteral econutrition (EEN) group〕. After gastrostomy, rats in each group were treated with the isocaloric and isonitrogenous nutritional formulas for 7 days, respectively. The morphology of ileum membrane was studied, and the quantities of IgA+, CD3+, CD4+ and CD8+ cells (each HP) of ileum membrane were determined. Results The villus height, crypt depth, mucosal thickness (except EN group) and villus surface area of ileum were increased in EN, EIN and EEN group compared with control group (P＜0.05), but there was no significant difference among the former three groups (P gt;0.05). The numbers of IgA+, CD3+, CD4+ and CD8+ cells were increased in EN, EIN and EEN group compared with control group (P＜0.05), and those numbers in EN group were lower than those in EIN and EEN group (P＜0.05). Conclusion EIN and EEN may improve intestine mechanical barrier function and promote restoration of small intestine mucous membrane barrier function in rats. EIN and EEN also improve intestine immune barrier function and strengthen its immune function.

Citation： YU Lijian,XU Liang,YAO Hui,WEI Renzhi,QI Xiaolong. Effects of Enteral Immunonutrition and Econutrition on Intestinal Mucosa Barrier Function in Wounded Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(11): 794-798. doi: Copy

1.	Bengmark S. Immunonutrition: role of biosurfactants, fiber, and probiotic bacteria [J]. Nutrition, 1998; 14(7-8)∶585.
2.	李江，刘斌，侯宇, 等. 腹部外科术后早期肠内营养的临床研究与应用 [J]. 中国普外基础与临床杂志, 2006; 13(3)∶355.
3.	李宁. 临床营养的新概念：免疫营养 [J]. 中国实用外科杂志， 2001； 21(1)∶7.
4.	Bengmark S. Econutrition and health maintenance——a new concept to prevent GI inflammation, ulceration and sepsis [J]. Clin Nutr, 1996; 15(1)∶1.
5.	孙少川, 郑春宁, 曲飞, 等. 重组人生长激素对大鼠肠缺血再灌注后肠壁组织T淋巴细胞亚群和浆细胞的影响 [J]. 中华实验外科杂志, 2005; 22(4)∶447.
6.	彭乃宝. 维护肠道屏障功能的重要性及措施 [J]. 齐齐哈尔医学院学报, 2005; 26(10)∶1182.
7.	孙敬平，马洪升. 应激状态下肠屏障功能的变化及其机理的研究进展 [J]. 中国普外基础与临床杂志, 2005; 12（3）∶314.
8.	武正炎主编. 普通外科手术并发症预防与处理 [M]. 第1版. 北京: 人民军医出版社， 2002∶25~28.
9.	Pscheidl E, Schywalsky M, Tschaikowsky K, et al. Fish oil-supplemented parenteral diets normalize splanchnic blood flow and improve killing of translocated bacteria in a low-dose endotoxin rat model [J]. Crit Care Med, 2000; 28(5)∶1489.
10.	胡建昆. 外科手术后早期肠内营养 [J]. 中国普外基础与临床杂志, 2003; 10(4)∶407.
11.	吴国豪. 肠道屏障功能 [J]. 肠外与肠内营养, 2004; 11（1）∶44.
12.	Popovic PJ, Zeh HJ 3rd, Ochoa JB. Arginine and immunity [J]. J Nutr, 2007; 137(6 Suppl 2)∶1681S.
13.	吴国豪. 特殊营养物质与肠内营养 [J]. 世界华人消化杂志, 2000; 12(8)∶1398.
14.	次仁桑珠, 王征夏, 张辉. 免疫营养剂对器官移植的作用 [J]. 中国普外基础与临床杂志, 2007; 14（2）∶243.
15.	Efron D, Barbul A. Role of arginine in immunonutrition [J]. J Gastroenterol, 2000; 35(Suppl 12)∶20.
16.	Tuohy KM, Probert HM, Smejkal CW, et al.Using probiotics and prebiotics to improve gut health [J]. Drug Discov Today， 2003; 8(15)∶692.
17.	Doron S, Gorbach SL. Probiotics: their role in the treatment and prevention of disease [J]. Expert Rev Anti Infect Ther, 2006; 4(2)∶261.
18.	Thorlacius H, Nobaek S, Wang XD, et al. Lactobacilli attenuate bacteremia and endotoxemia associated with severe intra-abdominal infection [J]. Surgery, 2003; 134(3)∶467.
19.	Medici M, Vinderola CG, Weill R, et al. Effect of fermented milk containing probiotic bacteria in the prevention of an enteroinvasive Escherichia coli infection in mice [J]. J Dairy Res, 2005; 72(2)∶243.
20.	宗莉, 杨立明, 张传卫, 等. 益生菌及其细胞组分的免疫调节作用 [J]. 微生物学杂志, 2006； 26(2)∶92.

1. Bengmark S. Immunonutrition: role of biosurfactants, fiber, and probiotic bacteria [J]. Nutrition, 1998; 14(7-8)∶585.
2. 李江，刘斌，侯宇, 等. 腹部外科术后早期肠内营养的临床研究与应用 [J]. 中国普外基础与临床杂志, 2006; 13(3)∶355.
3. 李宁. 临床营养的新概念：免疫营养 [J]. 中国实用外科杂志， 2001； 21(1)∶7.
4. Bengmark S. Econutrition and health maintenance——a new concept to prevent GI inflammation, ulceration and sepsis [J]. Clin Nutr, 1996; 15(1)∶1.
5. 孙少川, 郑春宁, 曲飞, 等. 重组人生长激素对大鼠肠缺血再灌注后肠壁组织T淋巴细胞亚群和浆细胞的影响 [J]. 中华实验外科杂志, 2005; 22(4)∶447.
6. 彭乃宝. 维护肠道屏障功能的重要性及措施 [J]. 齐齐哈尔医学院学报, 2005; 26(10)∶1182.
7. 孙敬平，马洪升. 应激状态下肠屏障功能的变化及其机理的研究进展 [J]. 中国普外基础与临床杂志, 2005; 12（3）∶314.
8. 武正炎主编. 普通外科手术并发症预防与处理 [M]. 第1版. 北京: 人民军医出版社， 2002∶25~28.
9. Pscheidl E, Schywalsky M, Tschaikowsky K, et al. Fish oil-supplemented parenteral diets normalize splanchnic blood flow and improve killing of translocated bacteria in a low-dose endotoxin rat model [J]. Crit Care Med, 2000; 28(5)∶1489.
10. 胡建昆. 外科手术后早期肠内营养 [J]. 中国普外基础与临床杂志, 2003; 10(4)∶407.
11. 吴国豪. 肠道屏障功能 [J]. 肠外与肠内营养, 2004; 11（1）∶44.
12. Popovic PJ, Zeh HJ 3rd, Ochoa JB. Arginine and immunity [J]. J Nutr, 2007; 137(6 Suppl 2)∶1681S.
13. 吴国豪. 特殊营养物质与肠内营养 [J]. 世界华人消化杂志, 2000; 12(8)∶1398.
14. 次仁桑珠, 王征夏, 张辉. 免疫营养剂对器官移植的作用 [J]. 中国普外基础与临床杂志, 2007; 14（2）∶243.
15. Efron D, Barbul A. Role of arginine in immunonutrition [J]. J Gastroenterol, 2000; 35(Suppl 12)∶20.
16. Tuohy KM, Probert HM, Smejkal CW, et al.Using probiotics and prebiotics to improve gut health [J]. Drug Discov Today， 2003; 8(15)∶692.
17. Doron S, Gorbach SL. Probiotics: their role in the treatment and prevention of disease [J]. Expert Rev Anti Infect Ther, 2006; 4(2)∶261.
18. Thorlacius H, Nobaek S, Wang XD, et al. Lactobacilli attenuate bacteremia and endotoxemia associated with severe intra-abdominal infection [J]. Surgery, 2003; 134(3)∶467.
19. Medici M, Vinderola CG, Weill R, et al. Effect of fermented milk containing probiotic bacteria in the prevention of an enteroinvasive Escherichia coli infection in mice [J]. J Dairy Res, 2005; 72(2)∶243.
20. 宗莉, 杨立明, 张传卫, 等. 益生菌及其细胞组分的免疫调节作用 [J]. 微生物学杂志, 2006； 26(2)∶92.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Enteral Immunonutrition and Econutrition on Intestinal Mucosa Barrier Function in Wounded Rats

Abstract Full text Figures/Tables Video References Cited by

Format

Content