Semi-Quantitative Analysis for Human fxyd6 Gene of Cholangiocarcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

1.General Hospital of Airforce of People’s Liberation Army, Beijing 100036, China;;
2.General Hospital of People’s Liberation Army, Beijing 100853;;
3.Academy of Military Medical Sciences, Beijing 100853, ChinaCorresponding Author： XIAO Mei, E-mail： Fengweiairforce@hotmail.com;

Corresponding author：

Keywords：

CholangiocarcinomaHuman fxyd6 geneIon transportBioinformatics Polymerase chain reaction

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Objective To validate the different expressions of human fxyd6 gene between normal bile duct tissues and malignant tumor tissues, and to observe the subcellular localization of human fxyd6 gene in human cholangiocarcinoma cells. MethodsThe different expressions between normal bile duct tissues and malignant tumor tissues were identified by RT-PCR. In situ polymerase chain reaction （IS-RT-PCR） was applied to detect the subcellular localization of fxyd6 gene in paraffin sections of human cholangiocarcinoma cells. Image analysis software was used to semiquantitatively determine the difference between normal and malignant tissues. ResultsHuman fxyd6 gene was highly expressed in cholangiocarcinoma tissues and lowly expressed in normal ones. There was a significant difference between the expressions of carcinoma cells and normal cells （P＜0.05）. IS-RT-PCR showed that fxyd6 gene localized in the kytoplasma of epithelial cells of human cholangiocarcinoma. ConclusionHuman fxyd6 gene may act as an essential component of the malignant transformation process in human cholangiocarcinoma.

Citation： XIAO Mei,ZHOU Ningxin,GAO Lijie,ZHANG Aiqun,GUO Wei,GAO Yabing,PENG Ruiyun,ZHANG Xiaodong,WAN Tao. Semi-Quantitative Analysis for Human fxyd6 Gene of Cholangiocarcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(2): 83-87. doi: Copy

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1. 万涛,倪彬,刘建伟,等.人胆管癌相关基因CCR-L2/FXYD6大片段克隆与鉴定[J].中华实验外科杂志,2003; 20(5):393.
2. Sweadner KJ,Rael E.The FXYD gene family of small ion transport regulators or channels:cDNA sequence,protein signature sequence,and expression[J].Genomics,2000; 68(1):41.
3. 申洪.免疫组织化学染色定量方法研究(Ⅲ)[J].中国组织化学与细胞化学杂志,1995; 4(1):90.
4. Kowdley GC,Ackerman SJ,Chen Z,et al.Anion,cation,and zwitterion selectivity of phospholemman channel molecules[J].Biophys J,1997; 72(1):141.
5. Minor NT,Sha Q,Nichols CG,et al.The gamma subunit of the Na,K-ATPase induces cation channel activity[J].Proc Natl Acad Sci USA,1998; 95(11):6521.
6. Crambert G,Geering K.FXYD proteins:new tissue-specific regulators of the ubiquitous Na,K-ATPase[J].Sci STKE,2003; 2003(166):RE1.
7. Garty H,Karlish SJ.Role of FXYD proteins in ion transport[J].Annu Rev Physiol,2006; 68:431.
8. Clark HF,Gurney AL,Abaya E,et al.The secreted protein discovery initiative (SPDI),a large-scale effort to identify novel human secreted and transmembrane proteins:a bioinformatics assessment[J].Genome Res,2003; 13(10):2265.
9. http://www.ncbi.nlm.nih.gov/Nucleotied/NCBI Nucleotide Data Web Page[DB].
10. Rijken AM,Hu J,Perlman EJ,et al.Genomic alterations in distal bile duct carcinoma by comparative genomic hybridization and karyotype analysis[J].Genes Chromosomes Cancer,1999; 26(3):185.
11. Mahlamki EH,Hglund M,Gorunova L,et al.Comparative genomic hybridization reveals frequent gains of 20q,8q,11q,12p,and 17q,and losses of 18q,9p,and 15q in pancreatic cancer[J].Genes Chromosomes Cancer,1997; 20(4):383.
12. Gorunova L,Hglund M,Andrén-Sandberg A,et al.Cytogenetic analysis of pancreatic carcinomas:intratumor heterogeneity and nonrandom pattern of chromosome aberrations[J].Genes Chromosomes Cancer,1998; 23(2):81.
13. Chen YJ,Chen PJ,Lee MC,et al.Chromosomal analysis of hepatic adenoma and focal nodular hyperplasia by comparative genomic hybridization[J].Genes Chromosomes Cancer,2002; 35(2):138.
14. Pack SD,Karkera JD,Zhuang Z,et al.Molecular cytogenetic fingerprinting of esophageal squamous cell carcinoma by comparative genomic hybridization reveals a consistent pattern of chromosomal alterations[J].Genes Chromosomes Cancer,1999; 25(2):160.
15. Chen YJ,Ko JY,Chen PJ,et al.Chromosomal aberrations in nasopharyngeal carcinoma analyzed by comparative genomic hybridization[J].Genes Chromosomes Cancer,1999; 25(2):169.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Semi-Quantitative Analysis for Human fxyd6 Gene of Cholangiocarcinoma

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