Detection of Differential Expression of Serum Protein in Peripheral Blood by SELDI-TOF-MS Technology in Cholangiocarcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

.!.Department of Hepatobiliary Gastrointestinal Disease，The Second Artillery General Hospital of The PLA，Beijing100088，China;;
2 .D epartmentof H epatobiliary，TheG eneralH ospitalo fT heP LA，Beijing1 00853，Chi-、;;
3. Institute of Cell Biology, College of Li介Science，Beijing Normal University，Bejing 100875，ChinaCorresponding Author;;
ZHOUNing-xin，E-mail:zhounx301)163. com;

Corresponding author：

Keywords：

B iled uctm alignancy ,Tu morm arker ,Proteomics ,Surfacee nhancedl aserd esorption八onization-time of flight-mass spectrometry

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective Toa nalyzed ifferentialp roteine xpressiono fc holangiocarcinomai np eripheralb loodb y
proteomics technology, and to investigate the significance of proteomics technology in early diagnosis of bile duct
malignancy.M ethods Serum proteinf rom 58p atientsw ithc holangiocarcinomaa nd5 8c ontrols( 20p atientsw ith
cholecystolithiasis and 38 healthy people) were detected by surface enhanced laser desorption/ionization-time of
flight-mass spectrometry (SELDI-TOF-MS). Ciphergen protein chip software was used to identify proteinic spectra.
R esults Comparedw itht hes pectrao fs erum proteini nc ontrolg roup,t herew ere1 0d ifferentiallye xpressed
proteins in bile duct carcinoma group, among which three proteins with relative molecular masses of 5. 900 X 10’，
9.08 0X 1 0’a nd1 1.86 3X 1 0’w ereu p-regulated( P lt;1 0-’)ands evenp roteinsw ithr elativem olecularm asseso f
6.9 59X 1 0’，14.0 00X 1 0’，14.1 29X 1 0’，14.3 02X 1 0’，17.5 57X 10’，17.6 90X 1 0’a nd2 8.5 52X 1 0’w ered ownregulated
(P lt; 10-’)。The average concentration of protein with the relative molecular mass of 11. 863 X 10’ in
cholangiocarcinoma group was eight times more than that in controls group. At the stage I of cholangiocarcinoma,
thee xpressiono fp roteinp ointw itha r elativem olecularm asso f5 .90 0X1 0’w ass ignificantlyh ighert hant hosep atients
at the stage III and stage fV (P lt;10-’)，while there were no statistical difference of expression between diffeent clinical stages for the other 9 proteins points. And there were no significant expression differences of the above
10 proteins between the patients with and without jaundice following cholangiocarcinoma. Instead, another three
proteinsw ithr elativem olecularm asseso f7 .25 5X 1 03，12.36 4X 1 0’a nd1 5.8 73X 1 03w ered etectedt oh aved ifferentp
roteine xpressions.A nda llo fth em showedh ighe xpressionsin j aundiceg roup( P lt;10-5).C onclusion There
are remarkable differences of the expressions of serum proteins in peripheral blood in patients with cholangiocarcinoma.
T hep roteinp ointw itha r elativem olecularm asso f1 1.86 3X 1 0’m ayb ea p otentialb iomarkerfo re arlyd iagnosis
of cholangiocarcinoma

Citation： CHENJunzhou,ZHOUNingzin,LIUBoz,ZHOUD inghua,XIAOXueyuan,HED acheng. Detection of Differential Expression of Serum Protein in Peripheral Blood by SELDI-TOF-MS Technology in Cholangiocarcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(2): 88-91. doi: Copy

1.	<><><><><><><><><> [1]孙学军,石景森,何平.影响胆管癌切除术后的预后因素分析[J].中国普外基础与临床杂志,2006; 13(1):85.
2.	周宁新,黄志强.肝门部胆管癌103例外科治疗远期疗效的评析[J].中华外科杂志,1997; 35(11):649.
3.	Ward U,Primrose JN,Finan PJ,et al.The use of tumour markers CEA,CA-195 and CA-242 in evaluating the response to chemotherapy in patients with advanced colorectal cancer[J].Br J Cancer,1993; 67(5):1132.
4.	Somiari RI,Somiari S,Russell S,et al.Proteomics of breast carcinoma[J].J Chromatogr B Analyt Technol Biomed Life Sci,2005; 815(1-2):215.
5.	Malik G,Ward MD,Gupta SK,et al.Serum levels of an isoform of apolipoprotein A-Ⅱ as a potential marker for prostate cancer[J].Clin Cancer Res,2005; 11(3):1073.
6.	Pan YZ,Xiao XY,Zhao D,et al.Application of surface-enhanced laser desorption/ionization time-of-flight-based serum proteomic array technique for the early diagnosis of prostate cancer[J].Asian J Androl,2006; 8(1):45.
7.	von Eggeling F,Davies H,Lomas L,et al.Tissue-specific microdissection coupled with ProteinChip array technologies:applications in cancer research[J].Biotechniques,2000; 29(5):1066.
8.	Hara T,Honda K,Ono M,et al.Identification of 2 serum biomarkers of renal cell carcinoma by surface enhanced laser desorption/ionization mass spectrometry[J].J Urol,2005; 174(4 Pt 1):1213.
9.	Vlahou A,Schellhammer PF,Mendrinos S,et al.Development of a novel proteomic approach for the detection of transitional cell carcinoma of the bladder in urine[J].Am J Pathol,2001; 158(4):1491.
10.	Wang JX,Zhang B,Yu JK,et al.Application of serum protein fingerprinting coupled with artificial neural network model in diagnosis of hepatocellular carcinoma[J].Chin Med J (Engl),2005; 118(15):1278.
11.	Honda K,Hayashida Y,Umaki T,et al.Possible detection of pancreatic cancer by plasma protein profiling[J].Cancer Res,2005; 65(22):10613.
12.	徐文鸿,陈益定,胡跃,等.激光解析电离-飞行时间质谱技术和CM10蛋白质芯片检测术前大肠癌分期的意义[J].中华肿瘤杂志,2006; 28(10):753.
13.	黄志强.肝门部胆管癌的外科治疗与发展[J].中国普外基础与临床杂志,2003; 10(5):425.
14.	钟春生,张倩茹.多肿瘤标志物蛋白芯片检测系统在肿瘤诊断中的应用[J].安徽医药,2004; 8(2):109.
15.	Wiesner A.Detection of tumor markers with ProteinChip technology[J].Curr Pharm Biotechnol,2004; 5(1):45.

1. <><><><><><><><><> [1]孙学军,石景森,何平.影响胆管癌切除术后的预后因素分析[J].中国普外基础与临床杂志,2006; 13(1):85.
2. 周宁新,黄志强.肝门部胆管癌103例外科治疗远期疗效的评析[J].中华外科杂志,1997; 35(11):649.
3. Ward U,Primrose JN,Finan PJ,et al.The use of tumour markers CEA,CA-195 and CA-242 in evaluating the response to chemotherapy in patients with advanced colorectal cancer[J].Br J Cancer,1993; 67(5):1132.
4. Somiari RI,Somiari S,Russell S,et al.Proteomics of breast carcinoma[J].J Chromatogr B Analyt Technol Biomed Life Sci,2005; 815(1-2):215.
5. Malik G,Ward MD,Gupta SK,et al.Serum levels of an isoform of apolipoprotein A-Ⅱ as a potential marker for prostate cancer[J].Clin Cancer Res,2005; 11(3):1073.
6. Pan YZ,Xiao XY,Zhao D,et al.Application of surface-enhanced laser desorption/ionization time-of-flight-based serum proteomic array technique for the early diagnosis of prostate cancer[J].Asian J Androl,2006; 8(1):45.
7. von Eggeling F,Davies H,Lomas L,et al.Tissue-specific microdissection coupled with ProteinChip array technologies:applications in cancer research[J].Biotechniques,2000; 29(5):1066.
8. Hara T,Honda K,Ono M,et al.Identification of 2 serum biomarkers of renal cell carcinoma by surface enhanced laser desorption/ionization mass spectrometry[J].J Urol,2005; 174(4 Pt 1):1213.
9. Vlahou A,Schellhammer PF,Mendrinos S,et al.Development of a novel proteomic approach for the detection of transitional cell carcinoma of the bladder in urine[J].Am J Pathol,2001; 158(4):1491.
10. Wang JX,Zhang B,Yu JK,et al.Application of serum protein fingerprinting coupled with artificial neural network model in diagnosis of hepatocellular carcinoma[J].Chin Med J (Engl),2005; 118(15):1278.
11. Honda K,Hayashida Y,Umaki T,et al.Possible detection of pancreatic cancer by plasma protein profiling[J].Cancer Res,2005; 65(22):10613.
12. 徐文鸿,陈益定,胡跃,等.激光解析电离-飞行时间质谱技术和CM10蛋白质芯片检测术前大肠癌分期的意义[J].中华肿瘤杂志,2006; 28(10):753.
13. 黄志强.肝门部胆管癌的外科治疗与发展[J].中国普外基础与临床杂志,2003; 10(5):425.
14. 钟春生,张倩茹.多肿瘤标志物蛋白芯片检测系统在肿瘤诊断中的应用[J].安徽医药,2004; 8(2):109.
15. Wiesner A.Detection of tumor markers with ProteinChip technology[J].Curr Pharm Biotechnol,2004; 5(1):45.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Detection of Differential Expression of Serum Protein in Peripheral Blood by SELDI-TOF-MS Technology in Cholangiocarcinoma

Abstract Full text Figures/Tables Video References Cited by

Format

Content