Effects of Newcastle Disease Virus and Adriamycin on Surface Structure and Actin of Hepatocellular Carcinoma Cell Lines SMMC-7721_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

1. S urgical Department of Urban Uni t , General Hos pi tal of Cheng du Command , Cheng du 610017 , China;;
2. Department of General S urgery , General Hos pi tal of Cheng du Command , Cheng du 610083 , China;

Corresponding author：

Keywords：

Newcastle disease virus,Adriamycin,Hepatocellular carcinoma cell,Actin,Ca2+, CD44,Intercellular adhesion molecule-1,Surface structure

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【Abstract】Objective To compare the effects of newcastle disease virus (NDV) and adriamycin (ADM) on surface structure and actin of hepatocellular carcinoma cell lines SMMC-7721. Methods SMMC-7721 carcinoma cell lines were divided into 2 groups. NDV was added into one group, while ADM was added into the other group. The cells were then cultured at 5 time phases (8, 16, 24, 36 and 48 h). Intracellular actin and Ca2+ were examined by using immunofluorescence method. CD44 and intercellular adhesion molecule-1 (ICAM-1) were detected by using immunochemical method and flow cytometry, respectively. The change of cellular surface structure was observed by scan electron microscope. Results Cells gradually contracted and turned round over time. It was observed that actin was segmented and cells alignment became disordered. The mean fluorescence intensity of actin decreased in both groups, but it was obvious in NDV group. There were significant differences of fluorescence intensity between 2 groups at the phases of 16 h (P＜0.05), 24 h (P＜0.05), 36 h (P＜0.01) and 48 h (P＜0.05), except the one after 8 h. Intracellular Ca2+ concentration increased gradually in both groups, and the amplifications in NDV group were significantly higher at the phases of 24 h, 36 h and 48 h than those in ADM group (P＜0.01， P＜0.05 and P＜0.01, respectively). There were also differences at 8 and 16 h, but there were no statistical significance. The expression of CD44 in cells decreased. The mean fluorescence intensity of ICAM-1 raised gradually, and then came to peaking at 36 h, but there was no significant difference between two groups. All the above indices between different phases in the same group showed significant differences (P＜0.05). Conclusion Both NDV and ADM could make tumor cells degenerate and rupture, but the effect of NDV is more intensive. It could increase the fragility of cells and hasten the process of cell rupture. Disintegrated cancer cell and changes of adhesion molecule could lead cancer cells be identified, encapsulated, and killed by immune cells under static condition.

Citation： Z HAO Tiej un ,T IA N Fuz hou,L I Wei,B AO Chuanz hi,CH EN T ao,YI N Zhil iang .. Effects of Newcastle Disease Virus and Adriamycin on Surface Structure and Actin of Hepatocellular Carcinoma Cell Lines SMMC-7721. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(4): 240-244. doi: Copy

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2. 　吴孟超. 肝癌外科治疗的近期进展 [J]. 中国普外基础与临床杂志， 2006； 13(2)∶125.
3. 　Luna EJ, Hitt AL. Cytoskeleton——plasma membrane interactions [J]. Science，1992; 258(5084)∶955.
4. 　庄庆琪. 细胞骨架 [A]. 见: 陈惠黎主编. 生物大分子的结构和功能 [M]. 第1版. 上海: 上海医科大学出版社, 1999∶143~166.
5. 　Cunningham CC. Actin structural proteins in cell motility [J]. Cancer Metastasis Rev，1992; 11(1)∶69.
6. 　Lou W, Krill D, Dhir R, et al. Methylation of the CD44 metastasis suppressor gene in human prostate cancer [J]. Cancer Res，1999; 59(10)∶2329.
7. 　Allouche M, Charrad RS, Bettaieb A, et al. Ligation of the CD44 adhesion molecule inhibits drug-induced apoptosis in human myeloid leukemia cells [J]. Blood，2000; 96(3)∶1187.
8. 　Hgerkorp CM, Bilke S, Breslin T, et al. CD44-stimulated human B cells express transcripts specifically involved in immunomodulation and inflammation as analyzed by DNA microarrays [J]. Blood，2003; 101(6)∶2307.
9. 　牟兆新, 侯振江. CD44及其在肿瘤研究中的进展 [J]. 医学综述, 2006; 12(6)∶341.
10. 　Jothy S. CD44 and its partners in metastasis [J]. Clin Exp Metastasis，2003; 20(3)∶195.
11. 　Camp RL, Kraus TA, PuréE. Variations in the cytoskeletal interaction and posttranslational modification of the CD44 homing receptor in macrophages [J]. J Cell Biol，1991; 115(5)∶1283.
12. 　Seiter S, Arch R, Reber S, et al. Prevention of tumor metastasis formation by anti-variant CD44 [J]. J Exp Med，1993; 177(2)∶443.
13. 　　刘岑, 金伯泉. 黏附分子 [A]. 见: 金伯泉主编. 细胞和分子免疫学 [M]. 第1版. 西安: 世界图书出版公司， 1995∶33~66.
14. 　Lum AF, Green CE, Lee GR, et al. Dynamic regulation of LFA-1 activation and neutrophil arrest on intercellular adhesion molecule 1 (ICAM-1) in shear flow [J]. J Biol Chem，2002; 277(23)∶20660.
15. 　Green CE, Schaff UY, Sarantos MR, et al. Dynamic shifts in LFA-1 affinity regulate neutrophil rolling, arrest, and transmigration on inflamed endothelium [J]. Blood，2006; 107(5)∶2101.
16. 　王燕, 周力, 李利. 原发性肝癌sICAM-1, sP-selectin检测及意义 [J]. 中华医学研究杂志, 2004; 4(5)∶185.
17. 　潘勤, 李定国. 细胞间粘附分子-1与慢性肝病 [J]. 临床消化病杂志, 2002; 14(6)∶272.
18. 　Porgador A, Mandelboim O, Restifo NP, et al. Natural killer cell lines kill autologous beta2-microglobulin-deficient melanoma cells: implications for cancer immunotherapy [J]. Proc Natl Acad Sci USA，1997; 94(24)∶13140.
19. 　赵铁军，陈涛，田伏洲，等. 新城疫病毒对肿瘤浸润淋巴细胞的作用 [J]. 中国普外基础与临床杂志，2002；9(5)∶329.
20. 　赵铁军，田伏洲，尹志良，等. 新城疫病毒治疗肝癌10例 [J]. 世界华人消化杂志，1999；7(8)∶718.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Newcastle Disease Virus and Adriamycin on Surface Structure and Actin of Hepatocellular Carcinoma Cell Lines SMMC-7721

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