• 1. Department of Geriatrics, The First Affiliated Hospital of China Medical University, Shenyang 110001, China;;
  • 2. The Affiliated Health School of Shenyang Medical College, Shenyang 110043, China;
Advanced glycation end products; 　　Receptor; 　　Hyperplasia; 　　Aminoguanidine; 　　Vein graft
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Objective　To study the expression of receptor of advanced glycation end products (RAGE) in autogenous vein graft of streptozotocin induced diabetic rats and the inhibitory effects of aminoguanidine on intimal hyperplasia. Methods　Sixty male Sprague-Dawley rats were randomly divided into three groups: aminoguanidine group, distilled water group and control group. Autogenous vein graft models were established in all groups. Streptozotocin was injected into abdominal cavity to induce diabetes in both aminoguanidine group and distilled water group, and they were intragastric administrated with aminoguanidine or distilled water, respectively before and after transplantation. Specimens were collected from autogenous vein graft 7 days and 14 days after surgery to undergo histological examination. At the same time, the level of serum advanced glycation end products (AGE) was tested. Western blotting and immunohistochemistry were used to detect the protein expression of RAGE and NF-κB p65. RAGE and NF-κB p65 mRNA were measured by reverse transcription-PCR. Results　 The mRNA and protein expressions of RAGE, NF-κB p65, the level of serum AGE and the intimal thickness of vein graft in distilled water group increased in comparison with those in control group 7 days and 14 days after surgery （P＜0.05). The level of serum AGE, mRNA and protein expressions of NF-κB p65 and the intimal thickness of vein graft in aminoguanidine group were lower than those in distilled water group （P＜0.05), and showed no significant difference compared with control group （P＞0.05). Conclusion　The over-expression of RAGE in vein graft activats NF-κB in streptozotocin-induced diabetic rat, which has a close relation with intimal hyperplasia. Aminoguanidine can block the binding of AGE and RAGE by inhibiting the production of AGE, which will prevent intimal hyperplasia of vein graft.

Citation： WANG Xin,ZHANG Qiang,ZHANG Hongwei,HUO Xin,DU Huilian. Expression of Receptor of Advanced Glycation End Products and Effect of Aminoguanidine on Intimal Hyperplasia in Autogenous Vein Graft of Rats with Streptozotocin Induced Diabetes. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(6): 390-395. doi: Copy