• 1. Department of Thoracic Surgery, The General Hospital of Aerospace, Beijing 100076, China;;
  • 2. Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China;
RNA interference; 　　Short hairpin RNA; 　　Vein graft; 　　Intimal hyperplasia
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Objective　To construct vectors that express phosphatidylinositol-3-kinase, catalytic, beta polypeptide （PIK3cb） shRNA in eukaryon plasmid catalyzed by PI3K in rat, then test their effects on intimal hyperplasia in transplanted vein graft. Methods　One hundred and fifty SD rats were randomly divided into six groups （n=25, in each group）: blank (25% Pluronic F-127), shRNA-1, shRNA-2, 1/2 （shRNA-1+shRNA-2）, negative control (pGenesil-1 scramble shRNA) and positive control （wortmannin） group. The jugular vein in rats were interpositioned autologously into the common carotid artery. shRNA and 25% Pluronic F-127 were mixed and coated around the transplanted vein in three PIK3cb shRNA groups. Every 5 samples were removed according to the time point (1, 3, 7, 14 and 28 days after operation), respectively. The thickness of intima and neointima area were calculated and analyzed by computer system. The PCNA expression was detected by Western blot and SP immunohistochemistry. Results　The intimal thickness of three PIK3cb shRNA groups were lower than those in the blank group and negative control group on day 3, 7, 14, 28 after operation (P＜0.05); The neointima area in three PIK3cb shRNA groups (except shRNA-2 group on day 3, 7) began to decrease significantly from day one (P＜0.05). The protein expression of PCNA in three PIK3cb shRNA groups on day 3 after operation were decreased compared with blank group and negative group (P＜0.05). The percentage of the PCNA positive cells area in three PIK3cb shRNA groups were significantly lower than those in blank group and negative control group in each time point (P lt;0.05). There were no significant differences between blank and negative control group in different time points (P gt;0.05). Conclusion　The PIK3cb shRNA can effectively inhibit the proliferation of vascular smooth muscle cell, which may provide a new gene therapy for the prevention of vein graft restenosis after bypass grafting.

Citation： LIU Sujian ,SHEN Qingmin,LIU Hong,DENG Yongzhi,MA Jie. Effect of Double Sites shRNA Targeting at Phosphatidylinositol-3-Kinase Signaling on Intimal Hyperplasia in Transplanted Vein Graft. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(6): 396-401. doi: Copy