Comparison of The Injury of Intestines Induced by PAAF and ASPAAF in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Zhen ¹ , CHENG Ruochuan ² , FANG Xiang ¹ , ZHANG Yuetian ¹ , ZHAO Haijian ² , LI Jun ¹ , CHEN Yuxiang ¹

1.Department of General Surgery, The Peoples Hospital of Deyang City, Sichuan Province, Deyang 618000, China;;
2. Department of General Surgery, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, ChinaCorresponding Author： FANG Xiang, E-mail： fangxiang76111@163.com;

Corresponding author：

Keywords：

Pancreatitis associated ascitic fluid; Acute suppurative peritonitis associated ascitic fluidIntestinal mucosa injury; Tumor necrosis factor-α

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Objective 　 To explore the role and intrinsic mechanism of the injury of intestines induceded by pancreatitis associated ascitic fluid (PAAF) and acute suppurative peritonitis associated ascitic fluid (ASPAAF) in rats.
Methods 　Forty-eight Sprague-Dawley (SD) rats, male or female, were randomly divided into three groups averagely. The control group: 8 ml of normal saline (NS) was injected into the peritoneal cavity; the PAAF group: 8 ml of PAAF was injected into the peritoneal cavity; and the ASPAAF group: 8 ml of ASPAAF was injected into the peritoneal cavity. After peritoneal cavity injection, the rats were put to death in batches at 6 h and 12 h, eight rats per-batch. Levels of TNF-α and endotoxin in serum were measured. The activity of ATP enzyme and level of TNF-α in the intestinal tissues were measured. The pathological changes of intestines were observed by microscope.
Results 　The levels of TNF-α, endotoxin and the degree of injury of the intestines were markedly elevated and the activity of ATP enzyme of the intestinal tissues was decreased in the PAAF group and ASPAAF group compared with those in the control group (P＜0.05). The levels of TNF-α, endotoxin and the degree of injury of the intestines were markedly elevated and the activity of ATP enzyme of the intestinal tissues was decreased in the ASPAAF group compared with those in the PAAF group (P＜0.05).
Conclusion 　PAAF and ASPAAF can induce the injury of intestines, but the injury of intestines induced by ASPAAF is more serious.

Citation： LIU Zhen,CHENG Ruochuan,FANG Xiang,ZHANG Yuetian,ZHAO Haijian,LI Jun,CHEN Yuxiang. Comparison of The Injury of Intestines Induced by PAAF and ASPAAF in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(8): 555-559. doi: Copy

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1. 　方向，程若川，赵海剑，等． PAAF 诱导肠损伤的实验研究 [J]. 昆明医学院学报， 2005； 26(2)∶ 47.
2. 　 Lankisch PG, Ihse I. Bile-induced acute experimental pancreatitis [J].Scand J Gastroenterol, 1987； 22(3)∶ 257.
3. 　 Chiu CJ, McArdle AH, Brown R, et al. Intestinal mucosal lesion in low-flow states. Ⅰ. A morphological, hemodynamic, and metabolic reappraisal [J]. Arch Surg， 1970； 101(4)∶ 478.
4. 　 Ogle CK, Mao JX, Wu JZ, et al. The 1994 Lindberg Award. The production of tumor necrosis factor, interleukin-1, interleukin-6, and prostaglandin E2 by isolated enterocytes and gut macrophages: effect of lipopolysaccharide and thermal injury[J]. Burn Care Rehabil， 1994； 15(6)∶ 470.
5. 　Erwin PJ, Lewis IF, Dolan S, et al. Lipopolysaccharide binding protein in acute pancreatitis [J]. Crit Care Med， 2000； 28(1)∶ 104.
6. 　 Makhija R, Kingsnorth AN. Cytockine storm in acute pancreatitis[J]. J Hepatobiliary Pancreat Surg， 2002； 9(4)∶ 401.
7. 　 Davies MG, Hagen PO. Systemic inflammatory response syndrome [J]. Br J Surg, 1997； 84(7)∶ 920.
8. 　 Gomez-Cambronero LG, Sabater L, Pereda J, et al. Role of cytokines and oxidative stress in the pathophysiology of acute pancreatitis: therapeutical implications [J]. Curr Drug Targets Inflamm Allergy, 2002; 1(4)∶ 393.
9. 　 Pastor CM, Frossard JL. Are genetically modified mice useful for the understanding of acute pancreatitis? [J]. FASEB J, 2001; 15(6)∶ 893.
10. 　 Leindler L, Morschl E, Laszlo F, et al. Importance of cytokines, nitric oxide, and apoptosis in the pathological process of necrotizing pancreatitis in rats [J]. Pancreas, 2004; 29(2)∶ 157.
11. 　Ding LA, Li JS. Gut in diseases: physiological elements and their clinical significance[J]. World J Gastroenterol, 2003; 9(11)∶ 2385.
12. 　Liu LR, Xia SH. Role of platelet-activating factor in the pathogenesis of acute pancreatitis[J]. World J Gastroenterol, 2006; 12(4)∶ 539.
13. 　Tracey KJ, Beutler B, Lowry SF, et al. Shock and tissue injury induced by recombinant human cachectin [J]. Science, 1986; 234(4775)∶ 470.
14. 　李红光, 周总光, 雷松, 等. 人急性胰腺炎早期外周血单核细胞Toll样受体4表达变化 [J]. 中国普外基础与临床杂志, 2006； 13（2）∶191.
15. 　陈哲宇，齐清会，严律南. 细菌性腹膜炎诱导大鼠多器官功能不全综合征后结肠运动的变化和机理 [J]. 中国普外基础与临床杂志, 2006； 13（3）∶287.
16. 　刘殿刚, 孙家邦, 李非, 等. 胰腺炎相关腹水对大鼠胃肠动力的影响 [J]. 北京医学, 2005； 27（9）∶546.
17. 　Choudhry MA, Rana SN, Kavanaugh MJ. Impaired intestinal immunity and barrier function: a cause for enhanced bacterial translocation in alcohol intoxication and burn injury[J]. Alcohol, 2004; 33(3)∶ 199.
18. 　De-Souza DA, Greene LJ. Intestinal permeability and systemic infections in critically ill patients: effect of glutamine [J]. Crit Care Med, 2005; 33(5)∶ 1125.
19. 　Vliagoftis H, Befus AD, Hollenberg MD, et al. Airway epithelial cells release eosinophil survival-promoting factors (GM-CSF) after stimulation of proteinase-activated receptor 2 [J]. J Allergy Clin Immunol, 2001； 107(4)∶ 679.
20. 　van Pelt LJ, Huisman MV, Weening RS, et al. A single dose of granulocyte-macrophage colony-stimulating factor induces systemic interleukin-8 release and neutrophil activation in healthy volunteers [J]. Blood, 1996； 87(12)∶ 5305.
21. 　Frossard JL, Saluja AK, Mach N , et al. In vivo evidence for the role of GM-CSF as a mediated in acute pancreatitis-associated lung injury [J]. Am J Physiol Lung Cell Mol Physiol, 2002； 283(3)∶ L541.
22. 　ONeill S, ONeill AJ, Conroy E, et al. Altered caspase expression results in delayed neutrophil apoptosis in acute pancreatitis [J]. J Leukoc Biol, 2000； 68(1)∶ 15.
23. 　Botha AJ, Moore FA, Moore EE. Early neutrophil sequestration alter injury: a pathogenic mechanism for multiple organ failure [J]. J Trauma， 1995； 39(3)∶ 411.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Comparison of The Injury of Intestines Induced by PAAF and ASPAAF in Rats

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