Effect and Mechanism of Glutamine to The Aberrant Crypt Foci in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHENG Lei , TAN Guang , DONG Lei , LI Kejun , WANG Zhongyu.

Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, ChinaCorresponding Author： CHENG Lei, E-mail： iamchenglei@yahoo.com;

Corresponding author：

Keywords：

Aberrant crypt foci; Glutamine; CD44; Intercellular adhesion molecule-1

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Objective 　To explore the effect and mechanism of glutamine to the aberrant crypt foci (ACF) in rat injured by acetic acid.
Methods 　Thirty Wistar rats were averagely divided into three groups: control group, acetic acid group and glutamine group. The colon of the rat was infused with 1% acetic acid. Started to gavage with glutamate two days after modeling glutamine group. The injured colons were studied after fourteen days with light and scanning electronic microscope. Paraffin sections of specimens were prepared and stained with HE. The colon crypts were isolated by HCl digestion method. The expressions of CD44 and ICAM-1 in the epithelial cell of the large intestine mucosa were detected by immunohistochemistry method.
Results 　On the days of 14， the number of ACF in the glutamine group were remarkably decreased as compared with that of the acetic acid group and a branch-like. The expressions of ICAM-1 and CD44 (every 1 000 cells) were 302.1±30.1 and 298.6±28.3 in glutamine group, 223.6±23.5 and 221.5±28.6 in control group, 198.5±19.5 and 215.3±17.8 in acetic acid group, respectively. While the expressions of CD44 and ICAM-1 in intestine were increased remarkably in the glutamine group compared with the control group and acetic acid group (P＜0.05).
Conclusion 　 Glutamine could decrease the formation of the ACF injured by acetic acid. Increasing the expressions of CD44 and ICAM-1 may be one of the important factors to decrease the ACF.

Citation： CHENG Lei,TAN Guang,DONG Lei,LI Kejun,WANG Zhongyu.. Effect and Mechanism of Glutamine to The Aberrant Crypt Foci in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(8): 600-602. doi: Copy

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1. 　刘斌，郝志英. 谷氨酰胺在肿瘤治疗中的应用进展[Ｊ]. 肿瘤研究与临床， 2006； 18(3)∶ 207.
2. 　程蕾，来茂德. 一种新的结直肠癌癌前病变—畸形腺窝灶[Ｊ]. 临床与实验病理学杂志， 2001； 17(3)∶ 253.
3. 　Araki K, Kobayashi M, Ogata T. A scanning electron microscopic study of the three-dimensional configuration of isolated crypts from human colon adenomas [Ｊ]. Med Electron Microsc, 1994; 27(1)∶ 55.
4. 　Inoué T, Osatake H. A new drying method of biological specimens for scanning electron microscopy： the t-butyl alcohol freeze-drying method [Ｊ]. Arch Histol Cytol, 1988; 51(1)∶ 53.
5. 　Moon C, Jeong CW, Kim H, et al. Expression of CD44 adhesion molecule in rat testis with ischemia/reperfusion injury [Ｊ]. J Vet Med Sci, 2006; 68(7)∶ 761.
6. 　程湘，程天民. CD44分子的结构特征[Ｊ]. 国外医学·免疫学分册， 1998； 21 (4)∶ 185.
7. 　Alho AM, Underhill CB. The hyaluronate receptor is preferentially expressed on proliferating epithelial cells [Ｊ]. J Cell Biol, 1989; 108(4)∶ 1557.
8. 　夏国伟，史海安，周亚魁. 表皮生长因子、谷氨酰胺强化的全胃肠外营养对肠屏障功能和肠细菌移位的影响 [Ｊ]. 上海医科大学学报， 2000； 27(6)∶ 443.
9. 　刘宏, 吴雄飞, 李敛. 大鼠缺血再灌注损伤后肾组织ICAM-1表达及炎细胞浸润[Ｊ]. 第三军医大学学报， 2005； 27(15)∶ 1618.
10. 　刘敏丰，高志光，秦环龙. 生长激素和谷氨酰胺对脓毒症大鼠细胞因子和氨基酸代谢的影响 [Ｊ]. 中国普外基础与临床杂志， 2006； 13(5)∶ 535.
11. 　宫路路，韩喜春，姜伟栋. 谷氨酰胺对梗阻性黄疸大鼠可溶性白细胞介素2受体的影响 [Ｊ]. 中国普外基础与临床杂志， 2006； 13(4)∶ 445.
12. 　程雷，谭广，王忠裕，等. 谷氨酰胺对阻塞性黄疸大鼠免疫功能的影响 [Ｊ]. 中国普外基础与临床杂志， 2006； 13(1)∶ 71.
13. 　Afify A, Pang L, Howell L. Diagnostic utility of CD44 standard, CD44v6, and CD44v3-10 expression in adenocarcinomas presenting in serous fluids[Ｊ]. Appl Immunohistochem Mol Morphol, 2007; 15(4)∶ 446.
14. 　Cenesiz S, Devrim AK, Kamber U， et al. The effect of kefir on glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) levels in mice with colonic abnormal crypt formation (ACF) induced by azoxymethane (AOM) [Ｊ]. Dtsch Tierarztl Wochenschr， 2008; 115(1)∶15.
15. 　Ito T, Ohi S, Tachibana T, et al. Development of the mucosal vascular system in the distal colon of the fetal mouse [Ｊ]. Anat Rec (Hoboken)， 2008; 291(1)∶65.
16. 　Guebel DV, Torres NV. A computer model of oxygen dynamics in human colon mucosa： implications in normal physiology and early tumor development [Ｊ]. J Theor Biol， 2008; 250(3)∶389.
17. 　Cheung DY, Kim JI, Park YB, et al. Prolapsing mucosal polyps in the sigmoid colon: presenting with chronic abdominal cramping pain and colonic obstruction [Ｊ]. Intern Med, 2007; 46(20)∶1701.
18. 　Wang TD, Friedland S, Sahbaie P, et al. Functional imaging of colonic mucosa with a fibered confocal microscope for real-time in vivo pathology [Ｊ]. Clin Gastroenterol Hepatol， 2007; 5(11)∶1300.
19. 　Spek CA, ten Kate FJ, te Velde AA. Factor V Leiden and the etiology of inflammatory bowel disease [Ｊ]. Thromb Haemost， 2007; 98(3)∶670.
20. 　Matusiak D, Benya RV. CYP27A1 and CYP24 expression as a function of malignant transformation in the colon [Ｊ]. J Histochem Cytochem， 2007; 55(12)∶1257.
21. 　Zhaorigetu S, Sasaki M, Kato N. Consumption of sericin suppresses colon oxidative stress and aberrant crypt foci in 1, 2-dimethylhydrazine-treated rats by colon undigested sericin [Ｊ]. J Nutr Sci Vitaminol (Tokyo)， 2007; 53(3)∶297.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect and Mechanism of Glutamine to The Aberrant Crypt Foci in Rats

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