Significance and Expressions of P53 and CD34 in Rectal Cancer and Distal Mucosa_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

TU Xiaohuang ¹ , WANG Weizhong ² , WANG Lie ¹ , LI Chengjin ¹ , SONG Jingxiang ¹ , SONG Lina ¹ , ZHAO Chunlei ¹

1.Institute of General Surgery, Fuzhou General Hospital of Nanjing Military Command， Fuzhou 350025， China;;
2.Department of Gastroenterological Surgery, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, ChinaCorresponding Author： WANG Lie， E-mail： fzptwk@21cn.com;

Corresponding author：

Keywords：

Rectal cancer; Total mesorectal excision; P53; CD34; PET/CT; Immunohistochemistry

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To observe the expressions of P53 and CD34 in rectal cancer and distal mucosa and to explore the safe distal margin of radical surgery for rectal cancer at molecular pathologic level.
Methods 　Forty-five cases of rectal cancer were marked before operation， and then the cases were detected by PET/CT. P53 and CD34 expressions in rectal tissues were detected by immunohistochemistry technique.
Results 　P53 expression and microvessel density (MVD) in rectal cancer were significantly higher than those in distal mucosa， which in distal mucosa were decreased along the anal direction. P53 and CD34 were still found in the normal rectal tissue. P53 expression and MVD were not significantly different between in more than 1.5 cm distal rectal mucosa and in normal rectal tissue. Besides MVD was related to size of tumor in rectal cancer and distal 0.5 cm rectal mucosa tissue, P53 and CD34 in rectal cancer and distal mucosa rectal tissue were not associated with tumor diameter, stage and differentiation of rectal cancer.
Conclusion 　From the molecular pathologic view, the resection of 2.0 cm rectal distal tissue should be safe for excision of rectal cancer.

Citation： TU Xiaohuang,WANG Weizhong,WANG Lie,LI Chengjin,SONG Jingxiang,SONG Lina,ZHAO Chunlei. Significance and Expressions of P53 and CD34 in Rectal Cancer and Distal Mucosa. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(9): 641-645. doi: Copy

1.	Jeong SY, Chessin DB, Guillem JG. Surgical treatment of rectal cancer: radical resection [Ｊ]. Surg Oncol Clin N Am， 2006; 15(1)∶95.
2.	涂小煌，王烈主编. 直肠癌全直肠系膜切除术 [M]. 第1版. 福州：福建科学技术出版社, 2006∶122～130.
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4.	孙治君, 周鑫, 姚榛祥, 等. P-gp、nm23和p53联合检测判断乳腺癌预后的价值 [Ｊ]. 中国普外基础与临床杂志, 2005; 12(3)∶244.
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9.	张剑, 何生, 李茂德. 肝细胞癌中iNOS和p53蛋白的表达及其与肿瘤血管形成的关系 [Ｊ]. 中国普外基础与临床杂志, 2004; 11(5)∶396.
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11.	Kern A, Taubert H, Scheele J, et al. Association of p53 mutations, microvessel density and neoangiogenesis in pairs of colorectal cancers and corresponding liver metastases [Ｊ]. Int J Oncol, 2002; 21(2)∶243.
12.	Boxer GM, Tsiompanou E, Levine T, et al. Immunohistochemical expression of vascular endothelial growth factor and microvessel counting as prognostic indicators in node-negative colorectal cancer [Ｊ]. Tumour Biol, 2005; 26(1)∶1.
13.	Lebe B, Sarioglu S, Skmen S, et al. The clinical significance of p53, p21, and p27 expressions in rectal carcinoma [Ｊ]. Appl Immunohistochem Mol Morphol, 2005; 13(1)∶38.
14.	Ngoi SS, Staiano-Coico L, Godwin TA, et al. Abnormal DNA ploidy and proliferative patterns in superficial colonic epithelium adjacent to colorectal cancer [Ｊ]. Cancer, 1990; 66(5)∶953.
15.	潘志忠, 万德森, 张昌卿, 等. 大切片P53免疫组化染色对判断直肠癌远端扩散长度的临床意义 [Ｊ]. 癌症, 2004; 23(10)∶1199.
16.	范恩学, 孙海侠, 李爱丽. 直肠癌旁移行黏膜P21 P53蛋白表达及其对保肛术的影响 [Ｊ]. 山西医药杂志, 2005; 34(8)∶667.
17.	郑国强, 王怡, 陈香玲, 等. 直肠癌Dixon术后直肠下端切缘p53突变基因分析 [Ｊ]. 临床肿瘤学杂志, 2000; 5(4)∶229.
18.	邓海军, 李国新, 卿三华, 等. 直肠癌远侧癌旁黏膜p53基因突变及其蛋白表达 [Ｊ]. 实用癌症杂志, 2003; 18(2)∶170.
19.	Heald RJ, Husband EM, Ryall RD. The mesorectum in rectal cancer surgery——the clue to pelvic recurrence? [Ｊ]. Br J Surg, 1982; 69(10)∶613.
20.	Karanjia ND, Schache DJ, North WR, et al. ‘Close shave’ in anterior resection [Ｊ]. Br J Surg, 1990; 77(5)∶510.

1. 　Jeong SY, Chessin DB, Guillem JG. Surgical treatment of rectal cancer: radical resection [Ｊ]. Surg Oncol Clin N Am， 2006; 15(1)∶95.
2. 　涂小煌，王烈主编. 直肠癌全直肠系膜切除术 [M]. 第1版. 福州：福建科学技术出版社, 2006∶122～130.
3. 　Munro AJ, Lain S, Lane DP. P53 abnormalities and outcomes in colorectal cancer: a systematic review [Ｊ]. Br J Cancer， 2005; 92(3)∶434.
4. 　孙治君, 周鑫, 姚榛祥, 等. P-gp、nm23和p53联合检测判断乳腺癌预后的价值 [Ｊ]. 中国普外基础与临床杂志, 2005; 12(3)∶244.
5. 　Hilska M, Collan YU, O Laine VJ, et al. The significance of tumor markers for proliferation and apoptosis in predicting survival in coloretal cancer [Ｊ]. Dis Colon Rectum, 2005; 48(12)∶2197.
6. 　Zhou XD, Yu JP, Chen HX, et al. Expression of cellular FLICE-inhibitory protein and its association with p53 mutation in colon cancer [Ｊ]. World J Gastroenterol, 2005; 11(16)∶2482.
7. 　Salmon JS, Lockhart AC, Berlin J. Anti-angiogenic treatment of gastrointestinal malignancies [Ｊ]. Cancer Invest, 2005; 23(8)∶712.
8. 　Uzzan B, Nicolas P, Cucherat M, et al. Microvessel density as a prognostic factor in women with breast cancer: a systematic review of the literature and meta-analysis [Ｊ]. Cancer Res, 2004; 64(9)∶2941.
9. 　张剑, 何生, 李茂德. 肝细胞癌中iNOS和p53蛋白的表达及其与肿瘤血管形成的关系 [Ｊ]. 中国普外基础与临床杂志, 2004; 11(5)∶396.
10. 　Famulski W, Sulkowska M, Wincewicz A, et al. P53 correlates positively with VEGF in preoperative sera of colorectal cancer patients [Ｊ]. Neoplasma, 2006; 53(1)∶43.
11. 　Kern A, Taubert H, Scheele J, et al. Association of p53 mutations, microvessel density and neoangiogenesis in pairs of colorectal cancers and corresponding liver metastases [Ｊ]. Int J Oncol, 2002; 21(2)∶243.
12. 　Boxer GM, Tsiompanou E, Levine T, et al. Immunohistochemical expression of vascular endothelial growth factor and microvessel counting as prognostic indicators in node-negative colorectal cancer [Ｊ]. Tumour Biol, 2005; 26(1)∶1.
13. 　Lebe B, Sarioglu S, Skmen S, et al. The clinical significance of p53, p21, and p27 expressions in rectal carcinoma [Ｊ]. Appl Immunohistochem Mol Morphol, 2005; 13(1)∶38.
14. 　Ngoi SS, Staiano-Coico L, Godwin TA, et al. Abnormal DNA ploidy and proliferative patterns in superficial colonic epithelium adjacent to colorectal cancer [Ｊ]. Cancer, 1990; 66(5)∶953.
15. 　潘志忠, 万德森, 张昌卿, 等. 大切片P53免疫组化染色对判断直肠癌远端扩散长度的临床意义 [Ｊ]. 癌症, 2004; 23(10)∶1199.
16. 　范恩学, 孙海侠, 李爱丽. 直肠癌旁移行黏膜P21 P53蛋白表达及其对保肛术的影响 [Ｊ]. 山西医药杂志, 2005; 34(8)∶667.
17. 　郑国强, 王怡, 陈香玲, 等. 直肠癌Dixon术后直肠下端切缘p53突变基因分析 [Ｊ]. 临床肿瘤学杂志, 2000; 5(4)∶229.
18. 　邓海军, 李国新, 卿三华, 等. 直肠癌远侧癌旁黏膜p53基因突变及其蛋白表达 [Ｊ]. 实用癌症杂志, 2003; 18(2)∶170.
19. 　Heald RJ, Husband EM, Ryall RD. The mesorectum in rectal cancer surgery——the clue to pelvic recurrence? [Ｊ]. Br J Surg, 1982; 69(10)∶613.
20. 　Karanjia ND, Schache DJ, North WR, et al. ‘Close shave’ in anterior resection [Ｊ]. Br J Surg, 1990; 77(5)∶510.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Significance and Expressions of P53 and CD34 in Rectal Cancer and Distal Mucosa

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