Effect of Nidus Vespae on Lymphocyte Blastisation in Mixed Culture System of Lymphocyte and Pancreatic Islet_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WU Dequan ¹ , CHEN Ming ² , HUANG Yuenan ² , TAI Sheng ² , SUN Tiewei ² , ZHANG Xinchen ² , SU Huawei ²

Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;

Corresponding author：

Keywords：

Nidus vespae; Pancreatic islet; Mixed culture; Lymphocyte blastisation; Rejection

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Objective 　To investigate the effect of nidus vespae on lymphocyte blastisation in mixed culture system of lymphocyte and pancreatic islet.
Methods 　Solution of nidus vespae was extracted with ethanol from its herb. Rat lymphocyte and pig pancreatic islet were isolated and then were mixed together and cultured in incubators of 37 ℃ (concentration in volume: 5%CO2). Three different concentrations of extracted solution of nidus vespae （experimental group Ⅰ: 4.0 g/ml, group Ⅱ: 0.4 g/ml, group Ⅲ: 0.2 g/ml） were added to the mixed culture system of lymphocyte, respectively. Radioactive nuclide counts per minute were measured by 3H-thymdine test in order to examine the role of nidus vespae in inhibiting blatisation of lymphocyte, and the results were also compared with control group and CsA group, respectively.
Results 　The counts were all reduced significantly (P＜0.001) in 3 experimental groups compared with control group (group Ⅰ 45.3%, group Ⅱ 29.6%and group Ⅲ 9.2%). It also showed that the inhibitory effect became ber in higher concentration but still weaker than that in CsA group (80.7%).
Conclusion 　Nidus vespae could inhibit the blastisation of lymphocyte in mixed culture system of lymphocyte and pancreatic islet, and the effect increased as the the concentration increased, which may suggest that nidus vespae could suppress the rejection induced by T cells.

Citation： WU Dequan,CHEN Ming,HUANG Yuenan,TAI Sheng,SUN Tiewei,ZHANG Xinchen,SU Huawei. Effect of Nidus Vespae on Lymphocyte Blastisation in Mixed Culture System of Lymphocyte and Pancreatic Islet. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(2): 168-170. doi: Copy

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1. 　李旭，刘长庭，宋满景．蜂胶对慢性阻塞性肺疾病患者肺泡巨噬细胞胞浆游离钙浓度及其生成细胞因子的影响 [J]. 中国临床康复， 2005； 9（3）∶158.
2. 　黄跃南，吴德全，单世光，等．成年猪和大鼠胰岛分离纯化的实验研究 [J]. 哈尔滨医科大学学报， 2000; 34(4)∶249.
3. 　Calafiore R, Calcinaro F, Basta G, et al.A method for the massive separation of highly purified, adult porcine islets of Langerhans [J]. Metabolism， 1990; 39(2)∶175.
4. 　马继红，叶海军，赵俭，等. 蜂胶益胃胶囊治疗胃脘痛35例临床观察 [J]. 锦州医学院学报， 1995； 16（4）∶58.
5. 　朱彤, 吕效吾. 蜂胶在医学中的应用前景 [J]. 中国养蜂, 1997; 51(5)∶35.
6. 　Shizuru JA, Ramakrishnan S, Hunt T, et al. Inhibition of rat mixed lymphocyte pancreatic islet cultures with anti-Ia immunotoxin [J]. Transplantation， 1986; 42(6)∶660.
7. 　Zekorn T, Endl U, Horcher A, et al. Mixed lymphocyte islet culture for assessment of immunoprotection by islet microencapsulation [J]. Transplant Proc， 1995; 27(6)∶3362.
8. 　武鸿翔. 露蜂房中化学成分的研究与临床应用概况 [J]. 云南中医中药杂志， 2001; 22(3)∶29.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Nidus Vespae on Lymphocyte Blastisation in Mixed Culture System of Lymphocyte and Pancreatic Islet

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