Effect of Mesentery Imbedding Chemotherapy on The Healing of Colonic Stoma in Dog_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHU Wenyi ¹ , FAN Yuezu ² , YAN Bo ¹ , BAO Yanyi ¹ , HOU Kun ¹

Department of General and Laparoscopic Surgery, Shanghai Punan Hospital, Shanghai 200125, China;

Corresponding author：

Keywords：

Imbedding chemotherapy; Sustained release 5-fluorouracil; Pathology; Drug concentration

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Objective 　To investigate the effect of imbedding chemotherapy of sustained release of 5-fluorouracil on the healing of colonic stoma in dog.
Methods 　Twenty-eight adult hybrid dogs were randomly divided into chemotherapy group （n=22) and control group (n=6). The canine sigmoid colon were firstly detached and then anastomosed via median abdominal incision, 200 mg sustained release of 5-fluorouracil was imbedded in the mesentery 1.0-1.5 cm away from colonic stoma in chemotherapy group, whereas the control substance was injected into the dogs in control group. Tissue samples were collected from mesentery and stomas on 3, 5, 7, 10 and 15 days after operation, respectively, in order to observe the healing of stoma. The drug concentrations in the stoma and in the tissues that were 0, 1, 3, 5, 7, 10 and 15 cm away from the imbedding point were also measured by high performance liquid chromatography
method at different phases.
Results 　The tissues from colonic stoma only showed inflammatory reaction at early stage, with no necrosis and cellular degeneration. It was observed that the stoma healed basically on the tenth day after operation. The drug concentrations in the tissues gradually decreased at the range of 0-15 cm over time, but all of which were higher than the anti-tumor effective concentration （0.10 μg/g）.
Conclusion 　The imbedding chemotherapy of sustained release of 5-fluorouracil in mesentery has little effect on the healing of stoma, and it could remain an effective anti-tumor concentration in a period of time.

Citation： ZHU Wenyi,FAN Yuezu,YAN Bo,BAO Yanyi,HOU Kun. Effect of Mesentery Imbedding Chemotherapy on The Healing of Colonic Stoma in Dog. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(2): 192-195. doi: Copy

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1. 　张森, 万德森，潘志忠, 等. 结直肠癌肝转移的多因素分析 [J]. 中华肿瘤杂志, 2002; 24(4)∶367.
2. 　万德森. 直肠癌辅助放化疗的选择 [J]. 中国普外基础与临床杂志, 2003; 10(2)∶92.
3. 　Archer S, Gray B. Intraperitoneal 5-fluorouracil infusion for treatment of both peritoneal and liver micrometastases [J]. Surgery, 1990; 108(3)∶502.
4. 　何庆泗，姜希宏，寿楠海，等. 直肠癌周粘膜下注射抗癌药物对癌组织病理学形态及增殖核抗原表达影响的研究 [J]. 中华普通外科杂志， 1999； 14（4）∶251.
5. 　许健健，储成顶，周浩炳, 等. 局部药代动力学的药物浓度时空方程初探 [J]. 中国新药杂志, 2003； 12(4)∶284.
6. 　Chu CD, Tong XP, Xu JJ, et al. Study on local plasma drug concentration-space-time equation at the local embedding [J]. Asian J Drug Metab Pharmac, 2003; 3(1)∶25.
7. 　Peters GJ, Lankelma J, Kok RM, et al. Prolonged retention of high concentrations of 5-fluorouracil in human and murine tumors as compared with plasma [J]. Cancer Chemother Pharmacol, 1993; 31(4)∶269.
8. 　 Sugarbaker PH. A perspective on clinical research strategies in carcinoma of the large bowel [J]. World J Surg, 1991; 15(5)∶609.
9. 　韩晓燕，卫洪波，连建学，等. 5-氟尿嘧啶不同给药途径的药代动力学研究和临床应用 [J]. 中国新药杂志, 1999； 8(1)∶20.
10. 　Graf W, Weiber S, Glimelius B, et al. Influence of 5-fluorouracil and folinic acid on colonic healing: an experimental study in the rat [J]. Br J Surg, 1992; 79(8)∶825.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Mesentery Imbedding Chemotherapy on The Healing of Colonic Stoma in Dog

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