Study on The Effect of Estradiol on Proliferation of Diverse Mammary Primary Cells in Vitro_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Zhi , HUANG Tao , HAN Mingqiang , TIAN Yuan.

Department of Breast and Thyroid Center, Union Hospital, Wuhan 430022, China;

Corresponding author：

Keywords：

Breast cancer; Estrogen; Primary culture; Study in vitro

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To understand the effect of estradiol in different concentrations on proliferation of diverse mammary primary cells in vitro.
Methods 　The primary cells of cancer tissue, the adjacent tissue to tumors and normal mammary tissue from patiens with breast cancer were obtained using collagenase digesting method. All the tissue samples were cultivated in vitro, and were given estradiol in different concentrations. The effect of estradiol on the proliferation of those primary cells was measured by MTT.
Results 　Estradiol remarkedly promoted the proliferation of primary cells of cancer tissue and peritumor tissue in vitro, whose ER expression were positive. Whereas, the promotion effect of estradiol on the proliferation of normal mammary primary cells was relatively weak, and there was no correlation between the promotion effect with the expression of ER in cancer tissue.
Conclusion 　The risks of occurrence and relapse of breast cancer would increase significantly when the concentration of estradiol is no less than 103 pmol/L in vivo.

Citation： LI Zhi,HUANG Tao,HAN Mingqiang,TIAN Yuan.. Study on The Effect of Estradiol on Proliferation of Diverse Mammary Primary Cells in Vitro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(3): 255-258. doi: Copy

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1. Travis RC, Key TJ. Oestrogen exposure and breast cancer risk [J]. Breast Cancer Res, 2003; 5(5)∶239.
2. Pike MC, Spicer DV, Dahmoush L, et al. Estrogens, progestogens, normal breast cell proliferation, and breast cancer risk [J]. Epidemiol Rev, 1993; 15(1)∶17.
3. Folkerd EJ, Martin LA, Kendall A, et al. The relationship between factors affecting endogenous oestradiol levels in postmenopausal women and breast cancer [J]. J Steroid Biochem Mol Biol, 2006; 102(1-5)∶250.
4. Rockhill B, Byrne C, Rosner B, et al. Breast cancer risk prediction with a logincidence model: evaluation of accuracy [J]. J Clin Epidemiol, 2003; 56(9)∶856.
5. Kabuto M, Akiba S, Stevens RG, et al. A prospective study of estradiol and breast cancer in Japanese women [J]. Cancer Epidemiol Biomarkers Prev, 2000; 9(6)∶575.
6. Cotterchio M, Kreiger N, Theis B, et al. Hormonal factors and the risk of breast cancer according to estrogenand progesteronereceptor subgroup [J]. Cancer Epidemiol Biomarkers Prev, 2003; 12(10)∶1053.
7. Jensen J, Kitlen JW, Briand P, et al. Effect of antiestrogens and aromatase inhibitor on basal growth of the human breast cancer cell line MCF7 in serumfree medium [J]. J Steroid Biochem Mol Biol, 2003; 84(4)∶469.
8. Kuperwasser C, Chavarria T, Wu M, et al. Reconstruction of functionally normal and malignant human breast tissues in mice [J]. Proc Natl Acad Sci USA, 2004; 101(14)∶4966.
9. Pechoux C, Gudjonsson T, RonnovJessen L, et al. Human mammary luminal epithelial cells contain progenitors to myoepithelial cells [J]. Dev Biol, 1999; 206(1)∶88.
10. Collaborative Group on Hormonal Factors in Breast Cancer. Breast cancer and hormone replacement therapy: collaborative reanalysis of data from 51 epidemiological studies of 52, 705 women with breast cancer and 108, 411 women without breast cancer [J]. Lancet, 1997; 350(9084)∶1047.
11. Eerola H, Aittomaki K, AskoSeljavaara S, et al. Hereditary breast cancer and handling of patients at risk [J]. Scand J Surg, 2002; 91(3)∶280.
12. 郭贵龙，张筱骅，姚榛祥. 选择性COX2抑制剂尼美舒利对大鼠乳腺癌的影响及机理研究 [J]. 中国普外基础与临床杂志, 2005; 12（3）∶219.
13. Clarke RB, Howell A, Potten CS, et al. Dissociation between steroid receptor expression and cell proliferation in the human breast [J]. Cancer Res, 1997; 57(22)∶4987.
14. 龙启明，杨金巧，陈晓理，等. 125碘标记17α乙烯雌二醇3醋酸酯在荷人乳腺癌裸鼠体内生物学分布 [J]. 中国普外基础与临床杂志, 1999; 6(5)∶266.
15. Khan SA, Rogers MA, Obando JA, et al. Estrogen receptor expression of benign breast epithelium and its association with breast cancer [J]. Cancer Res, 1994; 54(4)∶993.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on The Effect of Estradiol on Proliferation of Diverse Mammary Primary Cells in Vitro

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