Effect of 103Pd Radioactive Stent on The Expression of Fas Gene and Its Relation with Apoptosis of Bile Duct Cancer Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HE Guijin ¹ , YU Faqiang ¹ , GAO Qinyi ² , XU Shuhe ³ , GAO Hong ⁴ , JIANG Weiguo ⁵ , JIANG tao ⁴ , DAI Xianwei ¹ , DAI Chaoliu ¹

Department of General Surgery, Shengjin Hospital, China Medical University, Shenyang 110004, China;

Corresponding author：

Keywords：

103Pd radioactive stent; γ-radiation; Fas gene; Apoptosis

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Objective 　To research the effect of γ-radiation released from 103Pd radioactive stent on the expression of Fas gene and its relation with apoptosis of bile duct cancer cells lines.
Methods 　 The cancer cells of bile duct were dissociated into suspension in culture flasks, and the number of cells was counted by hemacytometry. The suspension was then stored in 2 ml freezing tubes in the density of 1×105/ml. They were set in two stents: general stent （general stent group） and 103Pd radioactive stent (103Pd stent group). The expression of Fas gene and apoptosis of bile duct cancer cells in general stent group and 103Pd stent group were analysed with immunohistochemistry technique and TUNEL method, respectively.
Results 　 The expression level of Fas gene in 103Pd stent group was significantly higher than that in general stent group （P＜0.05）, and the number of apoptotic cancer cells in 103Pd stent group was also significantly more than that in general stent group （P＜0.01）.
Conclusion 　There is a correlation between the expression of Fas gene and the apoptosis of bile duct cancer cells, which means that 103Pd radioactive stent may increase the expression of Fas gene and promote the apoptosis of cancer cells. It may be helpful for the further study of treatment for bile duct cancer using 103Pd radiative stent.

Citation： HE Guijin,YU Faqiang,GAO Qinyi,XU Shuhe,GAO Hong,JIANG Weiguo,JIANG tao,DAI Xianwei,DAI Chaoliu. Effect of 103Pd Radioactive Stent on The Expression of Fas Gene and Its Relation with Apoptosis of Bile Duct Cancer Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(3): 284-286. doi: Copy

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11.	Oshimi Y, Miyazaki S. Fas antigen-mediated DNA fragmentation and apoptotic morphologic changes are regulated by elevated cytosolic Ca2+ level [J]. J Immunol， 1995; 154(2)∶599.
12.	Venet F, Pachot A, Debard AL, et al. Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism [J]. J Immunol， 2006; 177(9)∶6540.
13.	Chmura SJ, Nodzenski E, Beckett MA, et al. Loss of ceramide production confers resistance to radiation-induced apoptosis [J]. Cancer Res， 1997; 57(7)∶1270.
14.	Gadbois DM, Crissman HA, Nastasi A, et al. Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays [J]. Radiat Res， 1996; 146(4)∶414.
15.	Petri S, Kiaei M, Wille E, et al. Loss of Fas ligand-function improves survival in G93A-transgenic ALS mice [J]. J Neurol Sci， 2006; 251(1-2)∶44.

1. Itamochi H, Yamasaki F, Sudo T, et al. Reduction of radiation-induced apoptosis by specific expression of Bcl-2 in normal cells [J]. Cancer Gene Ther， 2006; 13(5)∶451.
2. Peschel RE, Colberg JW, Chen Z, et al. Iodine 125 versus palladium 103 implants for prostate cancer: clinical outcomes and complications [J]. Cancer J， 2004; 10(3)∶170.
3. Grigoriu M, Palade R, Vasile D, et al. Technic, indications and contribution of choledochoscopy in the diagnosis and treatment of biliary diseases [J]. Chirurgia (Bucur)， 2003; 98(2)∶167.
4. Wiedermann JG, Marboe C, Amols H, et al. Intracoronary irradiation markedly reduces restenosis after balloon angioplasty in a porcine model [J]. J Am Coll Cardiol， 1994; 23(6)∶1491.
5. 李海民，窦科峰，李韧, 等. 肝癌组织中Fas和FasL mRNA表达水平的变化及其意义 [J]. 中国普外基础与临床杂志, 2005; 12（3）∶284.
6. Bittner RM, Crowe SF. The relationship between naming difficulty and FAS performance following traumatic brain injury [J]. Brain Inj， 2006; 20(9)∶971.
7. Drewa T, Wolski Z, Skok Z, et al. The FAS-related apoptosis signaling pathway in the prostate intraepithelial neoplasia and cancer lesions [J]. Acta Pol Pharm， 2006; 63(4)∶311.
8. Protonotariou E, Rizos D, Malamitsi-Puchner A, et al. Tissue polypeptide specific antigen and soluble Fas during normal pregnancy and early life [J]. In Vivo， 2006; 20(6B)∶901.
9. McVicker BL, Tuma DJ, Kubik JL, et al. Ethanol-induced apoptosis in polarized hepatic cells possibly through regulation of the Fas pathway [J]. Alcohol Clin Exp Res， 2006; 30(11)∶1906.
10. Weissman JL. Old problems, new techniques: the interventional radiologist and the lacrimal apparatus [J]. Radiology， 1999; 212(2)∶305.
11. Oshimi Y, Miyazaki S. Fas antigen-mediated DNA fragmentation and apoptotic morphologic changes are regulated by elevated cytosolic Ca2+ level [J]. J Immunol， 1995; 154(2)∶599.
12. Venet F, Pachot A, Debard AL, et al. Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism [J]. J Immunol， 2006; 177(9)∶6540.
13. Chmura SJ, Nodzenski E, Beckett MA, et al. Loss of ceramide production confers resistance to radiation-induced apoptosis [J]. Cancer Res， 1997; 57(7)∶1270.
14. Gadbois DM, Crissman HA, Nastasi A, et al. Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays [J]. Radiat Res， 1996; 146(4)∶414.
15. Petri S, Kiaei M, Wille E, et al. Loss of Fas ligand-function improves survival in G93A-transgenic ALS mice [J]. J Neurol Sci， 2006; 251(1-2)∶44.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of 103Pd Radioactive Stent on The Expression of Fas Gene and Its Relation with Apoptosis of Bile Duct Cancer Cells

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