Expressions of S-Phase Kinase-Associated Protein 2 and p27 Protein in Gastric Carcinoma and Pericancerous Tissues_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YAO Hongliang , YANG Zhulin , LI Yongguo , LIU Guowen

Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha 410011, China;

Corresponding author：

Keywords：

Gastric neoplasm; S-phase kinase-associated protein 2; p27 protein; Immunohistochemistry

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To study the expressions of SKP2 and p27 in gastric carcinoma and pericancerous tissues and to detect the relationship between their expressions and clinicopathological features.
Methods 　Forty-nine cases of gastric carcinoma spicemen and 20 cases of tissue adjacent to the carcinoma were cut and made into paraffin-embedded slices. The expressions of SKP2 and p27 were then detected by SP immunohistochemical method.
Results 　The positive expression rate and score of SKP2 were both significantly higher in the gastric carcinoma tissues than those in pericancerous tissues (P＜0.01), whereas those terms of p27 were higher in pericancerous tissues (P＜0.05, P＜0.01). It was observed that the pericancerous tissues with positive SKP2 expression or with negative p27 expression showed atypical hyperplasis ranging from moderate to severe degrees. The positive rate and score of SKP2 were significantly lower in the cases of infiltrating depth T1+T2， without-metastasis of lymph node, with-metastasis of the first site lymph node, and without-metastasis of distant organs than those in infiltrating depth T3+T4, with-metastasis of lymph node, with-metastasis of the second or third site lymph node and with-metastasis of distant organs in gastric carcinoma tissues (P＜0.05， P＜0.01), whereas the results were contrary for p27 (P＜0.01). There was a negative correlation between the score of SKP2 and that of p27 in gastric carcinoma tissues (r=-0.65, P＜0.01).
Conclusion 　The expressions of SKP2 and p27 may act as important biological markers to reflect carcinogenesis, progression, biological beheviors and prognosis of gastric carcinoma.

Citation： YAO Hongliang,YANG Zhulin,LI Yongguo,LIU Guowen. Expressions of S-Phase Kinase-Associated Protein 2 and p27 Protein in Gastric Carcinoma and Pericancerous Tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(3): 308-311. doi: Copy

1.	Harada K, Supriatno, Kawashima Y, et al. Down-regulation of S-phase kinase associated protein 2 (Skp2) induces apoptosis in oral cancer cells [J]. Oral Oncol， 2005; 41(6)∶623.
2.	Oliveira AM, Okuno SH, Nascimento AG, et al. Skp2 protein expression in soft tissue sarcomas [J]. J Clin Oncol， 2003; 21(4)∶722.
3.	Takanami I. The prognostic value of overexpression of Skp2 mRNA in non-small cell lung cancer [J]. Oncol Rep， 2005; 13(4)∶727.
4.	Li JQ, Wu F, Masaki T, et al. Correlation of Skp2 with carcinogenesis, invasion, metastasis, and prognosis in colorectal tumors [J]. Int J Oncol， 2004; 25(1)∶87.
5.	Shim EH, Johnson L, Noh HL, et al. Expression of the F-box protein SKP2 induces hyperplasia, dysplasia, and low-grade carcinoma in the mouse prostate [J]. Cancer Res， 2003; 63(7)∶1583.
6.	Tigli H, Buyru N, Dalay N. Molecular analysis of the p27/kip1 gene in breast cancer [J]. Mol Diagn， 2005; 9(1)∶17.
7.	Li Q, Murphy M, Ross J, et al. Skp2 and p27kip1 expression in melanocytic nevi and melanoma: an inverse relationship [J]. J Cutan Pathol， 2004; 31(10)∶633.
8.	Fukuchi M, Masuda N, Nakajima M, et al. Inverse correlation between expression levels of p27 and the ubiquitin ligase subunit Skp2 in early esophageal squamous cell carcinoma [J]. Anticancer Res， 2004; 24(2B)∶777.
9.	Shintani S, Li C, Mihara M, et al. Skp2 and Jab1 expression are associated with inverse expression of p27(KIP1) and poor prognosis in oral squamous cell carcinomas [J]. Oncology， 2003; 65(4)∶355.
10.	靖昌庆，李乐平，李昭. 直肠癌p27基因表达及血清转化生长因子β1水平的变化 [J]. 中国普外基础与临床杂志, 2005； 12(1)∶33.
11.	周彦明，李玉民，朱有全, 等. cyclin E和p27kip1蛋白在胆囊癌组织中的表达及其意义 [J]. 中国普外基础与临床杂志, 2004； 11(2)∶148.
12.	Radke S, Pirkmaier A, Germain D. Differential expression of the F-box proteins Skp2 and Skp2B in breast cancer [J]. Oncogene， 2005; 24(21)∶3448.
13.	Yokoi S, Yasui K, Mori M, et al. Amplification and overexpression of SKP2 are associated with metastasis of non-small-cell lung cancers to lymph nodes [J]. Am J Pathol， 2004; 165(1)∶175.
14.	Langner C, von Wasielewski R, Ratschek M, et al. Expression of p27 and its ubiquitin ligase subunit Skp2 in upper urinary tract transitional cell carcinoma [J]. Urology， 2004; 64(3)∶611.
15.	牛坚，陈澍周，钱海鑫. TGFβ1及p27在原发性胆囊癌中的表达及临床意义 [J]. 中国普外基础与临床杂志, 2004； 11（6）∶518.

1. Harada K, Supriatno, Kawashima Y, et al. Down-regulation of S-phase kinase associated protein 2 (Skp2) induces apoptosis in oral cancer cells [J]. Oral Oncol， 2005; 41(6)∶623.
2. Oliveira AM, Okuno SH, Nascimento AG, et al. Skp2 protein expression in soft tissue sarcomas [J]. J Clin Oncol， 2003; 21(4)∶722.
3. Takanami I. The prognostic value of overexpression of Skp2 mRNA in non-small cell lung cancer [J]. Oncol Rep， 2005; 13(4)∶727.
4. Li JQ, Wu F, Masaki T, et al. Correlation of Skp2 with carcinogenesis, invasion, metastasis, and prognosis in colorectal tumors [J]. Int J Oncol， 2004; 25(1)∶87.
5. Shim EH, Johnson L, Noh HL, et al. Expression of the F-box protein SKP2 induces hyperplasia, dysplasia, and low-grade carcinoma in the mouse prostate [J]. Cancer Res， 2003; 63(7)∶1583.
6. Tigli H, Buyru N, Dalay N. Molecular analysis of the p27/kip1 gene in breast cancer [J]. Mol Diagn， 2005; 9(1)∶17.
7. Li Q, Murphy M, Ross J, et al. Skp2 and p27kip1 expression in melanocytic nevi and melanoma: an inverse relationship [J]. J Cutan Pathol， 2004; 31(10)∶633.
8. Fukuchi M, Masuda N, Nakajima M, et al. Inverse correlation between expression levels of p27 and the ubiquitin ligase subunit Skp2 in early esophageal squamous cell carcinoma [J]. Anticancer Res， 2004; 24(2B)∶777.
9. Shintani S, Li C, Mihara M, et al. Skp2 and Jab1 expression are associated with inverse expression of p27(KIP1) and poor prognosis in oral squamous cell carcinomas [J]. Oncology， 2003; 65(4)∶355.
10. 靖昌庆，李乐平，李昭. 直肠癌p27基因表达及血清转化生长因子β1水平的变化 [J]. 中国普外基础与临床杂志, 2005； 12(1)∶33.
11. 周彦明，李玉民，朱有全, 等. cyclin E和p27kip1蛋白在胆囊癌组织中的表达及其意义 [J]. 中国普外基础与临床杂志, 2004； 11(2)∶148.
12. Radke S, Pirkmaier A, Germain D. Differential expression of the F-box proteins Skp2 and Skp2B in breast cancer [J]. Oncogene， 2005; 24(21)∶3448.
13. Yokoi S, Yasui K, Mori M, et al. Amplification and overexpression of SKP2 are associated with metastasis of non-small-cell lung cancers to lymph nodes [J]. Am J Pathol， 2004; 165(1)∶175.
14. Langner C, von Wasielewski R, Ratschek M, et al. Expression of p27 and its ubiquitin ligase subunit Skp2 in upper urinary tract transitional cell carcinoma [J]. Urology， 2004; 64(3)∶611.
15. 牛坚，陈澍周，钱海鑫. TGFβ1及p27在原发性胆囊癌中的表达及临床意义 [J]. 中国普外基础与临床杂志, 2004； 11（6）∶518.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions of S-Phase Kinase-Associated Protein 2 and p27 Protein in Gastric Carcinoma and Pericancerous Tissues

Abstract Full text Figures/Tables Video References Cited by

Format

Content