Effect of CO2-Insufflation on Invasion Capacity of Colon Cancer Cells in Vitro_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

MA Junjun , FENG Bo , ZHANG Yi , LU Aiguo , HU Weiguo , LI Jianwen , WANG Mingliang , ZHENG Minhua

Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai Minimally Invasive Surgery Center, Shanghai Institute of Digestive Surgery, Shanghai 200025, China;

Corresponding author：

Keywords：

Colorectal neoplasm; Artificial pneumoperitoneum; Cell adhesion molecule; Neoplasm metastasis

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To investigate the influence of CO2-insufflation pressure on invasion potential of the colon cancer cells.
Methods 　With an in vitro artificial pneumoperitoneum model, SW1116 human colon cancer cells were exposed to CO2-insufflation of 5 different pressure groups: 6, 9, 12, 15 mm Hg and control group, respectively for 1 h. The invasion capacities of SW1116 cells exposed to CO2-insufflation of 5 different pressure groups were detected by cell adhesion/invasion assay in vitro.
Results 　Immediately following exposure to 15 mm Hg CO2 insufflation, the invasion of SW1116 cells decreased significantly compared to the cells before exposure. At the 0 h time point, the cells exposed to 15 mm Hg were significantly less invasive than those exposed to the other insufflation pressure (P＜0.05), and the cells exposed to 6 mm Hg were more invasive than cells exposed to the other insufflation pressure (P＜0.05). And 72 h after exposed to CO2-insufflation, the differences between the pressure groups were not significant.
Conclusion 　CO2-insufflation induced a temporary change in the invasion capacity of cancer cells in vitro, higher pressure of CO2-insufflation inhibits the invasion potential.

Citation： MA Junjun,FENG Bo,ZHANG Yi,LU Aiguo,HU Weiguo,LI Jianwen,WANG Mingliang,ZHENG Minhua. Effect of CO2-Insufflation on Invasion Capacity of Colon Cancer Cells in Vitro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(5): 512-515. doi: Copy

1.	The Clinical Outcomes of Surgical Therapy Study Group. A comparison of laparoscopically assisted and open colectomy for colon cancer [J]. N Engl J Med， 2004; 350(20)∶2050.
2.	Guillou PJ, Quirke P, Thorpe H, et al. Short-term endpoints of conventional versus laparoscopic-assisted surgery in patients with colorectal cancer (MRC CLASICC trial): multicentre, randomised controlled trial [J]. Lancet， 2005; 365(9472)∶1718.
3.	Veldkamp R, Kuhry E, Hop WC, et al. Laparoscopic surgery versus open surgery for colon cancer: short-term outcomes of a randomised trial [J]. Lancet Oncol， 2005; 6(7)∶477.
4.	Yu Q, Stamenkovic I. Localization of matrix metalloproteinase 9 to the cell surface provides a mechanism for CD44-mediated tumor invasion [J]. Genes Dev， 1999; 13(1)∶35.
5.	郑民华，马君俊，冯波，等. CO2气腹对结肠癌细胞粘附能力影响及其与粘附分子的关系 [J]. 中国实用外科杂志， 2005； 20(11)∶668.
6.	Liu YJ, Yan PS, Li J, et al. Expression and significance of CD44s, CD44v6, and nm23 mRNA in human cancer [J]. World J Gastroenterol， 2005; 11(42)∶6601.
7.	Illemann M, Bird N, Majeed A, et al. MMP-9 is differentially expressed in primary human colorectal adenocarcinomas and their metastases [J]. Mol Cancer Res， 2006; 4(5)∶293.
8.	Li BH, Zhao P, Liu SZ, et al. Matrix metalloproteinase-2 and tissue inhibitor of metallo-proteinase-2 in colorectal carcinoma invasion and metastasis[J]. World J Gastroenterol， 2005; 11(20)∶3046.
9.	Karadag A, Fedarko NS, Fisher LW. Dentin matrix protein 1 enhances invasion potential of colon cancer cells by bridging matrix metalloproteinase-9 to integrins and CD44 [J]. Cancer Res， 2005; 65(24)∶11545.
10.	Jiang WG. E-cadherin and its associated protein catenins, cancer invasion and metastasis [J]. Br J Surg， 1996; 83(4)∶437.
11.	Kinsella AR, Green B, Lepts GC, et al. The role of the cell-cell adhesion molecule E-cadherin in large bowel tumour cell invasion and metastasis [J]. Br J Cancer， 1993; 67(5)∶904.
12.	Wlodarczyk J, Stolte M, Mueller J. E-cadherin, beta-catenin and stromelysin-3 expression in de novo carcinoma of the colorectum [J]. Pol J Pathol， 2001; 52(3)∶119.
13.	Wlodarczyk J, Bethke B, Mueller E, et al. A comparative study of E-cadherin and stromelysin-3 expression in de novo and ex adenoma carcinoma of the colorectum [J]. Virchows Arch， 2001; 439(6)∶756.
14.	车向明，王曙逢，樊林，等. E-cadherin反义寡核苷酸对肿瘤细胞浸润能力影响的研究 [J]. 中国普外基础与临床杂志, 2006; 13(2)∶191.
15.	Zhou YN, Xu CP, Han B, et al. Expression of E-cadherin and beta-catenin in gastric carcinoma and its correlation with the clinicopathological features and patient survival [J]. World J Gastroenterol， 2002; 8(6)∶987.
16.	Haier J, Nasralla M, Nicolson GL. Cell surface molecules and their prognostic values in assessing colorectal carcinomas [J]. Ann Surg， 2000; 231(1)∶11.
17.	范西红，尹格平. 直肠癌和大肠粘膜组织细胞间粘附分子-1的表达及其临床意义 [J]. 中国普外基础与临床杂志， 2000; 7(3)∶155.
18.	Ziprin P, Ridgway PF, Peck DH, et al. Laparoscopic enhancement of tumour cell binding to the peritoneum is inhibited by anti-intercellular adhesion molecule-1 monoclonal antibody [J]. Surg Endosc， 2003; 17(11)∶1812.
19.	马君俊，郑民华，冯波，等. CO2气腹对结肠癌细胞表面粘附分子表达影响的研究 [J]. 中华消化内镜杂志， 2006； 23（2）∶114.
20.	Gutt CN, Gessmann T, Schemmer P, et al. The impact of carbon dioxide and helium insufflation on experimental liver metastases, macrophages, and cell adhesion molecules [J]. Surg Endosc， 2003; 17(10)∶1628.

1. 　The Clinical Outcomes of Surgical Therapy Study Group. A comparison of laparoscopically assisted and open colectomy for colon cancer [J]. N Engl J Med， 2004; 350(20)∶2050.
2. 　Guillou PJ, Quirke P, Thorpe H, et al. Short-term endpoints of conventional versus laparoscopic-assisted surgery in patients with colorectal cancer (MRC CLASICC trial): multicentre, randomised controlled trial [J]. Lancet， 2005; 365(9472)∶1718.
3. 　Veldkamp R, Kuhry E, Hop WC, et al. Laparoscopic surgery versus open surgery for colon cancer: short-term outcomes of a randomised trial [J]. Lancet Oncol， 2005; 6(7)∶477.
4. 　Yu Q, Stamenkovic I. Localization of matrix metalloproteinase 9 to the cell surface provides a mechanism for CD44-mediated tumor invasion [J]. Genes Dev， 1999; 13(1)∶35.
5. 　郑民华，马君俊，冯波，等. CO2气腹对结肠癌细胞粘附能力影响及其与粘附分子的关系 [J]. 中国实用外科杂志， 2005； 20(11)∶668.
6. 　Liu YJ, Yan PS, Li J, et al. Expression and significance of CD44s, CD44v6, and nm23 mRNA in human cancer [J]. World J Gastroenterol， 2005; 11(42)∶6601.
7. 　Illemann M, Bird N, Majeed A, et al. MMP-9 is differentially expressed in primary human colorectal adenocarcinomas and their metastases [J]. Mol Cancer Res， 2006; 4(5)∶293.
8. 　Li BH, Zhao P, Liu SZ, et al. Matrix metalloproteinase-2 and tissue inhibitor of metallo-proteinase-2 in colorectal carcinoma invasion and metastasis[J]. World J Gastroenterol， 2005; 11(20)∶3046.
9. 　Karadag A, Fedarko NS, Fisher LW. Dentin matrix protein 1 enhances invasion potential of colon cancer cells by bridging matrix metalloproteinase-9 to integrins and CD44 [J]. Cancer Res， 2005; 65(24)∶11545.
10. 　 Jiang WG. E-cadherin and its associated protein catenins, cancer invasion and metastasis [J]. Br J Surg， 1996; 83(4)∶437.
11. 　Kinsella AR, Green B, Lepts GC, et al. The role of the cell-cell adhesion molecule E-cadherin in large bowel tumour cell invasion and metastasis [J]. Br J Cancer， 1993; 67(5)∶904.
12. 　Wlodarczyk J, Stolte M, Mueller J. E-cadherin, beta-catenin and stromelysin-3 expression in de novo carcinoma of the colorectum [J]. Pol J Pathol， 2001; 52(3)∶119.
13. 　Wlodarczyk J, Bethke B, Mueller E, et al. A comparative study of E-cadherin and stromelysin-3 expression in de novo and ex adenoma carcinoma of the colorectum [J]. Virchows Arch， 2001; 439(6)∶756.
14. 　车向明，王曙逢，樊林，等. E-cadherin反义寡核苷酸对肿瘤细胞浸润能力影响的研究 [J]. 中国普外基础与临床杂志, 2006; 13(2)∶191.
15. 　Zhou YN, Xu CP, Han B, et al. Expression of E-cadherin and beta-catenin in gastric carcinoma and its correlation with the clinicopathological features and patient survival [J]. World J Gastroenterol， 2002; 8(6)∶987.
16. 　Haier J, Nasralla M, Nicolson GL. Cell surface molecules and their prognostic values in assessing colorectal carcinomas [J]. Ann Surg， 2000; 231(1)∶11.
17. 　范西红，尹格平. 直肠癌和大肠粘膜组织细胞间粘附分子-1的表达及其临床意义 [J]. 中国普外基础与临床杂志， 2000; 7(3)∶155.
18. 　Ziprin P, Ridgway PF, Peck DH, et al. Laparoscopic enhancement of tumour cell binding to the peritoneum is inhibited by anti-intercellular adhesion molecule-1 monoclonal antibody [J]. Surg Endosc， 2003; 17(11)∶1812.
19. 　马君俊，郑民华，冯波，等. CO2气腹对结肠癌细胞表面粘附分子表达影响的研究 [J]. 中华消化内镜杂志， 2006； 23（2）∶114.
20. 　Gutt CN, Gessmann T, Schemmer P, et al. The impact of carbon dioxide and helium insufflation on experimental liver metastases, macrophages, and cell adhesion molecules [J]. Surg Endosc， 2003; 17(10)∶1628.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of CO2-Insufflation on Invasion Capacity of Colon Cancer Cells in Vitro

Abstract Full text Figures/Tables Video References Cited by

Format

Content