Chemotaxis on Umbilical Vein Endothelial Cells of SMMC7721 Hepatic Carcinoma Cells with Angiopoietin Gene Expression_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

PENG Lin , OU Jinrui , SUN Jian.

Department of Hepatobiliary Surgery, Guangdong Provincial People’s Hospital, Guangzhou 510080, China;

Corresponding author：

Keywords：

Angiopoietin Hepatic carcinoma cell line Chemotaxis

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【Abstract】ObjectiveTo observe the chemotactic role on umbilical vein endothelial cells of SMMC7721 hepatic carcinoma cells with angiopoietin gene expression in order to study the effects of angiopoietin on hepatocellular carcinoma angiogenesis.
MethodsAngiopoietin gene 1 (Ang-1) fragment and Ang-2 fragment was transfected into SMMC7721 liver carcinoma cell line by Lipofectamine induced gene transfection technique. The chemotactic role of SMMC7721 liver carcinoma cell line on umbilical vein endothelial cells was observed through microchemotaxis analysis.
ResultsThe chemotactic response of the Umbilical vein endothelial cells was obviously improved with Ang1 expression (P＜0.05). This effect seemed to be inhibited by Ang-1 antibody (P＜0.05). However, there was no difference of the chemotactic effects with or without Ang-2 expression (P gt;0.05).
ConclusionAng-1 is a chemotactic factor for vascular endothelial cell and a promoter for angiogenesis, whereas Ang-2 does not show obvious chemotactic role.

Citation： PENG Lin,OU Jinrui,SUN Jian.. Chemotaxis on Umbilical Vein Endothelial Cells of SMMC7721 Hepatic Carcinoma Cells with Angiopoietin Gene Expression. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(2): 154-157. doi: Copy

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1. antagonist for Tie2 that disrupts in vivo angiogenesis [Ｊ]. Science, 2002； 277(6)∶55.
2. 彭林，孙建，王卫东，等. 稳定表达促血管生成素基因肝癌细胞系的建立及产物的检测 [Ｊ]. 广东医学， 2003； 24(7)∶701.
3. Davis S, Yancopoulos GD. The angiopoietins: Yin and Yang in angiogenesis [Ｊ]. Curr Top Microbiol Immunol， 1999； 237(25)∶173.
4. Shalaby F, Rossant J, Yamaguchi TP, et al. Failure of bloodisland formation and vasculogenesis in Flk1deficient mice [Ｊ]. Nature, 2002； 376(6535)∶62.
5. Sato TN, Tozawa Y, Deutsch U, et al. Distinct roles of the receptor tyrosine kinases Tie1 and Tie2 in blood vessel formation [Ｊ]. Nature, 2001; 376(6535)∶70.
6. Levy AP, Levy NS, Wegner S, et al. Transcriptional regulation of the rat vascular endothelial growth factor gene by hypoxia [Ｊ]. J Biol Chem, 2003； 272(22)∶13333.
7. Davis S, Aldrich TH, Jones PF, et al. Isolation of angiopoietin1, a ligand for the TIE2 receptor, by secretiontrap expression cloning [Ｊ]. Cell, 1996； 87(7)∶1161.
8. Maisonpierre PC, Suri C, Jones PF, et al. Angiopoietin2: a natural.
9. Kim L, Kim JH, Ryu YS, et al. Regulation of angiopoietin2 mRNA levels in bovine microvascular endothelial cells by cytokines and hypox [Ｊ]. Circ Res, 2001； 83(8)∶852.
10. Orimura T, Sata M, Ueno T, et al. Increased expression of vascular endothelial growth factor is associated with tumor progression in hepatocellular carcinoma [Ｊ]. Human Pathol, 1998； 29(9)∶986.
11. Anaka S, Mori M, Sakamoto Y, et al. Biologic significance of angiopoietin2 expression in human hepatocellular carcinoma [Ｊ]. J Clin Invest, 2001； 103(3)∶341.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Chemotaxis on Umbilical Vein Endothelial Cells of SMMC7721 Hepatic Carcinoma Cells with Angiopoietin Gene Expression

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