Role of Tumor Necrosis FactorAlpha Monoclonal Antibody in Cell Apoptosis after Combined Liver and Kidney Transplantation in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HAN Shuling , YU Lixin.

Centre of Organ Transplantation， Nanfang Hospital of Southern Medical University, Guangzhou 510515, China;

Corresponding author：

Keywords：

Combined liver and kidney transplantation Cell apoptosis Tumor necrosis factor-alpha Monoclonal antibody

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【Abstract】Objective To investigate the potential role of tumor necrosis factoralpha (TNFα) in apoptosis after combined liver and kidney transplantation in rats.
MethodsEighty rats which had combined liver and kidney transplantation were randomly paired, were divided into study group (n=20) and control group (n=20). 40 ml of 4 ℃ sodium chloride and antiTNFα monoclonal antibody (30 ml was infused from portal veins to donated livers and 10 ml from renal arteries to donated kidneys) were infused to the study group (0.1 mg/kg weight),and the same quantity of 4 ℃ sodium chloride was infused the control group. Venous blood was drew at different phases after the transplantations to detect the function of kidney and liver. The level of TNFα and the cell apoptosis were detected in the transplanted tissues of liver and kidney by ELISA and terminal deoxynucleotidy transferase mediated dTUPbiotin nickend labeling (TUNEL).
ResultsThe levels of AST, ACT, Cr and BUN in the study group were significantly lower than those of the control group at the same phases (P＜0.05). The level of TNFα in the transplanted tissues of kidney and liver was also significantly lower as compared with those of control group. The cell apoptosis index of the transplanted tissues of kidney and liver was significantly smaller in the study group （P＜0.05）. There was no dramatically pathological change in the tissues of transplanted kidney and liver, which were treated with antiTNFα monoclonal antibody, and the structures are almost normal.
ConclusionAntiTNFα monoclonal antibody may reduce cell apoptosis and accelerate the restoration of function of liver and kidney after combined liver and kidney transplantation.

Citation： HAN Shuling,YU Lixin.. Role of Tumor Necrosis FactorAlpha Monoclonal Antibody in Cell Apoptosis after Combined Liver and Kidney Transplantation in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(3): 283-286. doi: Copy

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11.	康俊生. 肿瘤坏死因子与移植肝的保存再灌注损伤 [Ｊ]. 国外医学·生理病理科学与临床分册， 1998; 18（3）∶276.
12.	Falk R, Hacham M, Nyska A, et al. Induction of interleukin1 beta, tumour necrosis factoralpha and apoptosis in mouse organs by amphotericin B is neutralized by conjugation with arabinogalactan [Ｊ].
13.	Nagakawa Y, Williams GM, Zheng Q, et al. Oxidative mitochondrial DNA damage and deletion in hepatocytes of rejecting liver allografts in rats: role of TNFalpha [Ｊ]. Hepatology, 2005; 42(1)∶208.
14.	Misseri R, Meldrum DR, Dinarello CA, et al. TNFalpha mediates obstructioninduced renal tubular cell apoptosis and proapoptotic signaling [Ｊ]. Am J Physiol Renal Physiol, 2005; 288(2)∶F406.

1. J Antimicrob Chemother, 2005; 55(5)∶713.
2. Schemmer P, Liang R, Kincius M, et al. Taurine improves graft survival after experimental liver transplantation [J]. Liver Transpl， 2005; 11(8)∶950.
3. Donnahoo KK, Shames BD, Harken AH, et al. Review article: the role of tumor necrosis factor in renal ischemiareperfusion injury [J]. J Urol, 1999; 162(1)∶196.
4. 张毅，叶启发，周进学，等. 三七总皂苷对大鼠肝移植物细胞凋亡及Bcl2和Caspase3表达的影响 [J]. 中国普外基础与临床杂志, 2005; 12(2)∶113.
5. 韩述岭，于立新，马俊杰, 等. 抗肿瘤坏死因子α单克隆抗体减轻肾脏缺血再灌注损伤的实验研究 [J]. 第一军医大学学报, 2003; 23(4)∶332.
6. 孙煦勇，于立新，刘小友，等. 大鼠原位肝肾联合移植模型的建立 [J]. 第一军医大学学报, 2003; 23(6)∶ 614.
7. Tannapfel A, Kohlhaw K, Ebelt J, et al. Apoptosis and the expression of Fas and Fas ligand (FasL) antigen in rejection and reinfection in liver allograft specimens [J]. Transplantation, 1999; 67(7)∶1079.
8. Niculescu F, Niculescu T, Nguyen P, et al. Both apoptosis and complement membrane attack complex deposition are major features of murine acute graftvshost disease [J]. Exp Mol Pathol, 2005; 79(2)∶136.
9. 黄建钊，夏穗生. 对肝移植免疫耐受机理研究的思索 [J]. 中国普外基础与临床杂志, 2000; 7(4)∶261.
10. Colletti LM, Kunkel SL, Walz A, et al. The role of cytokine networks in the local liver injury following hepatic ischemia/reperfusion in the rat [J]. Hepatology, 1996; 23(3)∶506.
11. 康俊生. 肿瘤坏死因子与移植肝的保存再灌注损伤 [Ｊ]. 国外医学·生理病理科学与临床分册， 1998; 18（3）∶276.
12. Falk R, Hacham M, Nyska A, et al. Induction of interleukin1 beta, tumour necrosis factoralpha and apoptosis in mouse organs by amphotericin B is neutralized by conjugation with arabinogalactan [Ｊ].
13. Nagakawa Y, Williams GM, Zheng Q, et al. Oxidative mitochondrial DNA damage and deletion in hepatocytes of rejecting liver allografts in rats: role of TNFalpha [Ｊ]. Hepatology, 2005; 42(1)∶208.
14. Misseri R, Meldrum DR, Dinarello CA, et al. TNFalpha mediates obstructioninduced renal tubular cell apoptosis and proapoptotic signaling [Ｊ]. Am J Physiol Renal Physiol, 2005; 288(2)∶F406.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Role of Tumor Necrosis FactorAlpha Monoclonal Antibody in Cell Apoptosis after Combined Liver and Kidney Transplantation in Rats

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