Changes and Mechanism of Colon Motility of the Rats in Multiple Organ Dysfunction Syndrome Induced by Bacterial Peritonitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Zheyu ¹ , QI Qinghui ² , YAN Lnan. ¹

Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

Keywords：

Induce nitricoxide synthase Interleukin-6 Tumour necrosis factor-α Multiple organ dysfunction syndrome Colon motility

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

【Abstract】ObjectiveTo explore the changes of colon motility of the rats in multiple organ dysfunction syndrome （MODS） induced bacterial peritonitis and the effects of IL6, TNFα and induce nitricoxide synthase (iNOS) on colon motility.
MethodsWistar rats were divided into two groups, which were the control group and the MODS group. The number of stool, the amplitude changes of circular smooth muscle strip, the length of smooth muscle cell, and the changes of serum NO in two groups were observed. The expressions of IL6, TNFα and iNOS protein and IL6 mRNA, TNFα mRNA and iNOS mRNA in distal colon were investigated by using immunohistochemical methods and RTPCR.
ResultsThe numbers of stool and the amplitude in the MODS group were lower than those of the control group （P＜0.05）. The expressions of IL6, TNFα and iNOS were negative in the control group, while they were positive in the MODS group. IL6 mRNA,TNFα mRNA and iNOS mRNA were negative expression in the control group, but they were positive expression in the MODS group. The concentration of serum NO and the length of smooth muscle cells in the MODS group were higher than those of the control group (P＜0.01).
ConclusionColon motor dysfunction of the rats is related to the iNOS, IL6 and TNFα.

Citation： CHEN Zheyu,QI Qinghui,YAN Lnan.. Changes and Mechanism of Colon Motility of the Rats in Multiple Organ Dysfunction Syndrome Induced by Bacterial Peritonitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(3): 287-290. doi: Copy

1.	Lodato RF, Khan AR, Zembowicz MJ, et al. Roles of IL1 and TNF in the decreased ileal muscle contractility induced by lipopolysaccharide [Ｊ]. Am J Physiol, 1999; 276(6 Pt 1)∶G1356.
2.	陈哲宇, 齐清会. 多器官功能不全综合征大鼠结肠运动功能变化和机制的研究 [Ｊ]. 中华实验外科杂志, 2004; 21(3)∶341.
3.	Hierholzer C, Kalff JC, Chakraborty A, et al. Impaired gut contractility following hemorrhagic shock is accompaied by IL6 and GCSF production and neutrophil infiltration [Ｊ]. Dig Dis Sci, 2001; 46(2)∶230.
4.	Hierholzer C, Kalff JC, Billiar TR, et al. Induced nitric oxide promotes intestinal inflammation following hemorrhagic shock [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2004; 286(2)∶G225.
5.	De Winter BY. Study of the pathogenesis of paralytic ileus in animal models of experimentally induced postoperative and septic ileus [Ｊ]. Verh K Acad Geneeskd Belg, 2003; 65(5)∶293.
6.	Bani D, Baccari MC, Quattrone S, et al. Relaxin depresses small bowel motility through a nitric oxidemediated mechanism： Studies in mice [Ｊ]. Biol Reprod, 2002; 66(3)∶778.

1. Lodato RF, Khan AR, Zembowicz MJ, et al. Roles of IL1 and TNF in the decreased ileal muscle contractility induced by lipopolysaccharide [Ｊ]. Am J Physiol, 1999; 276(6 Pt 1)∶G1356.
2. 陈哲宇, 齐清会. 多器官功能不全综合征大鼠结肠运动功能变化和机制的研究 [Ｊ]. 中华实验外科杂志, 2004; 21(3)∶341.
3. Hierholzer C, Kalff JC, Chakraborty A, et al. Impaired gut contractility following hemorrhagic shock is accompaied by IL6 and GCSF production and neutrophil infiltration [Ｊ]. Dig Dis Sci, 2001; 46(2)∶230.
4. Hierholzer C, Kalff JC, Billiar TR, et al. Induced nitric oxide promotes intestinal inflammation following hemorrhagic shock [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2004; 286(2)∶G225.
5. De Winter BY. Study of the pathogenesis of paralytic ileus in animal models of experimentally induced postoperative and septic ileus [Ｊ]. Verh K Acad Geneeskd Belg, 2003; 65(5)∶293.
6. Bani D, Baccari MC, Quattrone S, et al. Relaxin depresses small bowel motility through a nitric oxidemediated mechanism： Studies in mice [Ｊ]. Biol Reprod, 2002; 66(3)∶778.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Changes and Mechanism of Colon Motility of the Rats in Multiple Organ Dysfunction Syndrome Induced by Bacterial Peritonitis

Abstract Full text Figures/Tables Video References Cited by

Format

Content