Effect of Multiple Thermotherapy on Proliferation Cycle and bcl-2 mRNA of HepG2 Cell_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

QIN Chunhong ¹ , LI Yongguo. ²

Department of General Surgery, The Second Affiliated Hospital of Nanhua University, Hengyang 421001, China;

Corresponding author：

Keywords：

Thermotherapy HepG2 cell Cell cycle bcl-2 gene bax gene

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【Abstract】ObjectiveTo investigate the proliferation rate of HepG2 cell after multiple thermotherapy and the possible reasons related to it.
MethodsAfter HepG2 cell were treaded by ten repeated cycles of heat exposure at 43 ℃ for 80 minutes twice a day, the doubling time of cell was analyzed， and the cell cycle, bcl2 mRNA and bax mRNA were detected.
ResultsThe proliferation rate of HepG2 cell which treated with heat speeded up, the percentage of G2 and S in cell cycle increased, the expression of bcl2 mRNA strengthened and the rate of bcl2/bax increased.
ConclusionThe speeded proliferation of HepG2 cell after multiple thermotherapy is related to its high percentage of DNA duplicated and dividing cell, strengthened expression of bcl2 mRNA and increased rate of bcl2/bax.

Citation： QIN Chunhong,LI Yongguo.. Effect of Multiple Thermotherapy on Proliferation Cycle and bcl-2 mRNA of HepG2 Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(3): 295-297下转304. doi: Copy

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Multiple Thermotherapy on Proliferation Cycle and bcl-2 mRNA of HepG2 Cell

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