Correlation Between Expression of hTERT mRNA and BRCA1 Protein in Breast Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Li , JIANG Jun , YANG Xinhua , FAN Linjun , CHEN Xianchun.

Breast Centre, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China;

Corresponding author：

Keywords：

Breast cancer Telomerase Breast cancer susceptibility gene 1

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【Abstract】ObjectiveTo investigate the expressions of hTERT mRNA and BRCA1 protein and to analyze the correlation between these two factors in breast cancer.
MethodsThe expression of hTERT mRNA was examined by reverse transcription polymerase chain reaction (RT-PCR). The expression of BRCA1 protein was examined by immunohistochemistry.
ResultsThe positive rates of hTERT mRNA and BRCA1 protein were 72.1%（31/43） and 34.9%（15/43） in breast cancer tissue, were 5.0%(2/40) and 77.5%(31/40) in paracancerous breast tissue respectively. Significant difference existed between breast cancer tissue and paracancerous breast tissue (P＜0.05). Significant negative correlation existed between the expression of BRCA1 protein and expression of hTERT mRNA （r=-0.995, P＜0.01）.
ConclusionThe expression of hTERT mRNA is upregulated in breast cancer, and expression of BRCA1 protein is downregulated in breast cancer. BRCA1 protein expression may be associated with expression of hTERT mRNA in breast cancer, which may be involved in the carcinogenesis of breast cancer.

Citation： CHEN Li,JIANG Jun,YANG Xinhua,FAN Linjun,CHEN Xianchun.. Correlation Between Expression of hTERT mRNA and BRCA1 Protein in Breast Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(4): 434-436. doi: Copy

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1. 李汛, 李玉民, 梁聚民，等. 端粒酶基因和DNA倍体在良恶性梗阻性黄疸中的表达 [Ｊ]. 中国普外基础与临床杂志， 2005； 12(4)∶ 343.
2. Gretarsdottir S, Sveinbjornsdottir S, Jonsson HH, et al. Localization of a susceptibility gene for common forms of stroke to 5q12 [Ｊ]. Am J Hum Genet, 2002; 70(3)∶593.
3. Bieche I, Nogues C, Paradis V, et al. Quantitation of hTERT gene expression in sporadic breast tumors with a realtime reverse transcriptionpolymerase chain reaction assay [Ｊ]. Clin Cancer Res， 2000； 6(2)∶452.
4. Miki Y, Swensen J, ShattuckEidens D, et al. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1 [Ｊ]. Science， 1994; 266(5182)∶66.
5. Li H, Lee TH, Avraham H. A novel tricomplex of BRCA1, Nmi, and cMyc inhibits cMycinduced human telomerase reverse transcriptase gene (hTERT) promoter activity in breast cancer [Ｊ]. J Biol Chem， 2002; 277(23)∶20965.
6. Venkitaraman AR. Cancer susceptibility and the functions of BRCA1 and BRCA2 [Ｊ]. Cell， 2002; 108(2)∶171.
7. Zhou C, Liu J. Inhibition of human telomerase reverse transcriptase gene expression by BRCA1 in human ovarian cancer cells [Ｊ]. Biochem Biophys Res Commun， 2003; 303(1)∶130.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Correlation Between Expression of hTERT mRNA and BRCA1 Protein in Breast Cancer

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