Expression and Functional Significance of Lung Resistance Protein in Pancreatic Adenocarcinoma Cell_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

GUO Junchao , YAN Changqing , LIAO Quan , ZHAO Yupei.

Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China;

Corresponding author：

Keywords：

Pancreatic neoplasmMultidrug resistanceAcquired drug resistance

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【Abstract】　Objective　To detect the expression of lung resistance protein (LRP) and investigate its significance in pancreatic carcinoma cell lines (SW1990, PCT-2, PCT-3, PCT-4, Aspc-1, Capan-1, Mia-PaCa-2 and Panc-1). Methods　Reverse transcription PCR (RT-PCR) and immunocytochemistry (ICC) were carried out to investigate the expression of LRP. Results　LRP mRNA was absent in PCT-2 cell line by RT-PCR. Mild to moderate expression level was found in other pancreatic carcinoma cell lines. PCT-4, Aspc-1 and Panc-1 presented the highest LRP mRNA expression level, in contrast, SW1990, PCT-3, Capan-1 and Mia-PaCa-2 showed moderate LRP mRNA expression. The median value was 0.56±0.33. LRP was further validated by ICC. Absent to weak protein expression of LRP was found in PCT-2 and PCT-3. Overexpressed LRP was present in SW1990, Capan-1 and Aspc-1, furthermore, the highest expression of LRP was found in Panc-1, Mia-PaCa-2 and PCT-4 cell lines. Conclusion　All these data showed that LRP might play an important role in multidrug resistance of pancreatic carcinoma.

Citation： GUO Junchao,YAN Changqing,LIAO Quan,ZHAO Yupei.. Expression and Functional Significance of Lung Resistance Protein in Pancreatic Adenocarcinoma Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(5): 502-505. doi: Copy

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12.	Mandoky L, Geczi L, Doleschall Z, et al. Expression and prognostic value of the lung resistance-related protein(LRP) in germ cell testicular tumors [J]. Anticancer Res, 2004; 24(2C)∶1097.
13.	Olson DP, Taylor BJ, La M, et al. The prognostic significance of P-glycoprotein, multidrug resistance-related protein 1 and lung resistance protein in pediatric acute lymphoblastic leukemia: a retrospective study of 295 newly diagnosed patients by the Children’s Oncology Group [J]. Leuk Lymphoma, 2005; 46(5)∶681.
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1. 　郭俊超. 多药耐药基因与胰腺癌化疗 [J]. 胰腺病学, 2002; 2(4)∶247.
2. 　张立阳, 赵玉沛, 廖　泉, 等. MDR1在胰腺癌细胞株中的表达及其意义 [J]. 中华实验外科杂志， 2003; 20(5)∶475.
3. 　张立阳, 赵玉沛, 吴元德，等. 胰腺癌阿霉素耐药细胞株SW1990/ADM的建立及其耐药机理研究 [J]. 中国普外基础与临床杂志， 2005; 12(1)∶46.
4. 　潘　博, 郭俊超, 廖　泉, 等. 吉西他滨与胰腺癌化疗耐药 [J]. 中国普外基础与临床杂志, 2005; 12(5)∶503.
5. 　郭俊超, 赵玉沛, 廖　泉, 等. 胰腺癌耐药细胞株SW1990/FU的建立、鉴定及生物学特性 [J]. 中国医学科学院学报, 2005; 27(5)∶592.
6. 　Berger W, Elbling L, Micksche M. Expression of the major va-ult protein LRP in human non-small-cell lung cancer cells: activation by short-term exposure to antineoplastic drugs [J].Int J Cancer, 2000; 88(2)∶293.
7. 　Fan K, Fan D, Cheng L, et al. Expression of multidrug resistance-related markers in gastric cancer [J]. Anticancer Res, 2000; 20(6C)∶4809.
8. 　Raidl M, Berger W, Schulte-Hermann R, et al. Expression of the lung resistance-related protein in human and rat hepatocarcinogenesis [J]. Am J Physiol Gastrointest Liver Physiol, 2002; 283(5)∶G1117.
9. 　Schneider J, Gonzalez-Roces S, Pollan M, et al. Expression of LRP and MDR1 in locally advanced breast cancer predicts axillary node invasion at the time of rescue mastectomy after induction chemotherapy [J]. Breast Cancer Res, 2001; 3(3)∶183.
10. 　Scheper RJ, Broxterman HJ, Scheffer GL, et al. Overexpression of a M(r) 110,000 vesicular protein in non-P-glycoprotein-mediated multidrug resistance [J]. Cancer Res, 1993; 53(7)∶1475.
11. 　Izquierdo MA, Scheffer GL, Flens MJ, et al. Major vault protein LRP-related multidrug resistance [J]. Eur J Cancer, 1996; 32A(6)∶979.
12. 　Mandoky L, Geczi L, Doleschall Z, et al. Expression and prognostic value of the lung resistance-related protein(LRP) in germ cell testicular tumors [J]. Anticancer Res, 2004; 24(2C)∶1097.
13. 　Olson DP, Taylor BJ, La M, et al. The prognostic significance of P-glycoprotein, multidrug resistance-related protein 1 and lung resistance protein in pediatric acute lymphoblastic leukemia: a retrospective study of 295 newly diagnosed patients by the Children’s Oncology Group [J]. Leuk Lymphoma, 2005; 46(5)∶681.
14. 　Izquierdo MA, Scheffer GL, Flens MJ, et al. Broad distribution of the multidrug resistance-related vault lung resistance protein in normal human tissues and tumors [J]. Am J Pathol, 1996; 148(3)∶877.
15. 　Liu B, Staren ED, Iwamura T, et al. Mechanisms of taxotere-related drug resistance in pancreatic carcinoma [J]. J Surg Res, 2001; 99(2)∶179.
16. 　Qadir M, O’Loughlin KL, Fricke SM, et al. Cyclosporin A is a broad-spectrum multidrug resistance modulator [J]. Clin Cancer Res, 2005; 11(6)∶2320.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression and Functional Significance of Lung Resistance Protein in Pancreatic Adenocarcinoma Cell

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