Effect of Verapamil on Apoptosis, Calcium and Expressions of bcl-2 and c-myc of Pancreatic Cells in Ischemia-Reperfusion Rat Model_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XING Jun , XU Ping , LIANG Desen , CHEN Yanbo , LI Aidong , SONG Chun , SONG Chunfang.

Department of General Surgery, The Cell Transplantation Key Laboratory of National Health Ministry, The First Affiliated Hospital of Harbin Medical University, Harbin 150001， China;

Corresponding author：

Keywords：

PancreasIschemia-reperfusion injuryCell apoptosisCalcium overloadVerapamil

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【Abstract】　Objective　To investigate the effect of verapamil on apoptosis, calcium and expressions of bcl-2 and c-myc of pancreatic cells in ischemia-reperfusion rat model. Methods　Wistar rats were randomly divided into three groups: control group （n=10）; ischemia-reperfusion group （n=10）; verapamil treatment group （n=10）. The anterior mesenteric artery and the celiac artery of rats in both ischemia-reperfusion group and verapamil treatment group were occluded for 15 min followed by 12-hour reperfusion. Verapamil （1 mg／kg） was injected via caudal vein to the rats in verapamil treatment group 15 min before occlusion and 1 hour after the initiation of reperfusion, respectively; and ischemia-reperfusion group was given the same volume of salient twice intravenously. Pancreatic tissues were collected from the dead rats after twelve hours since the reperfusion. The pathologic characters of pancreatic tissue were observed under light microscope; The level of calcium in the tissue was measured by atomic absorption spectrometer; TUNEL was used to detect apoptosis of pancreatic cells; and the expressions of c-myc and bcl-2 in the cells were also analyzed by immunohistochemistry technique and flow cytometry. Results　The pathologic change in verapamil treatment group was less conspicuous than that of ischemia-reperfusion group. Both the calcium level and the number of apoptotic cells in verapamil treatment group were less than those of ischemia-reperfusion group 〔（411.1±55.8） μg/g dry weight vs （470.9±31.9） μg/g dry weight, P＜0.05 and （9.5±2.9）% vs （18.4±3.1）% 〕, P＜0.05. After taking verapamil, the number of apoptotic cells decreased, whereas the expressions of bcl-2 and c-myc increased. The fluorescent indexes of bcl-2 and c-myc in verapamil treatment group were significantly higher than those of ischemia-reperfusion group (1.72±0.11 vs 1.41±0.07, P＜0.05; 1.76±0.19 vs 1.55±0.13, P＜0.05. Conclusion　Ischemia-reperfusion injury can induce apoptosis of pancreatic cells. Verapamil could protect the injured pancreatic tissue by reducing the level of calcium, stimulating the expressions of bcl-2 and c-myc and inhibiting apoptosis of pancreatic cells.

Citation： XING Jun,XU Ping,LIANG Desen,CHEN Yanbo,LI Aidong,SONG Chun,SONG Chunfang.. Effect of Verapamil on Apoptosis, Calcium and Expressions of bcl-2 and c-myc of Pancreatic Cells in Ischemia-Reperfusion Rat Model. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(5): 545-549. doi: Copy

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2. 　王恩华. 胰腺疾病 [A]. 见：杨光华主编. 病理学 [M]. 第5版. 北京: 人民卫生出版社, 2001∶221~223.
3. 　常　华, 严际慎, 王平瑜, 等．缺血-再灌注损伤对大鼠急性胰腺炎胰腺细胞凋亡的影响 [J]. 中国普外基础与临床杂志, 2005; 12(2)∶150.
4. 　薛东波, 邢　军, 张迎媚, 等. 胰腺缺血-再灌注损伤细胞凋亡及其相关因素的研究 [J]. 中华实验外科杂志, 2001; 18(2)∶125.
5. 　Smaili SS, Hsu YT, Youle RJ, et al. Mitochondria in Ca2+ signaling and apoptosis [J]. J Bioenerg Biomembr, 2000; 32(1)∶35.
6. 　Fauvel H, Marchetti P, Chopin C, et al. Differential effects of caspase inhibitors on endotoxin-induced myocardial dysfunction and heart apoptosis [J]. Am J Physiol Heart Circ Physiol, 2001; 280(4)∶1608.
7. 　Mattys EP, Mallo IJ, Iusi C, et al. Lipid alteration induced by renal ischemia pathogenic factor in membrane damage [J]. Transplantation, 1998; 65(2)∶155.
8. 　Polyak MM, Arrington BO, Stubenbord WT, et al. The influence of pulsatile preservation on renal transplantation in the 1990s [J]. Transplantation, 2000; 69(2)∶249.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Verapamil on Apoptosis, Calcium and Expressions of bcl-2 and c-myc of Pancreatic Cells in Ischemia-Reperfusion Rat Model

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