Protective Role of Recombinant Human Growth Hormone in Ischemic Reperfusion Injury of Rat Liver_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

RAN Jianghua ¹

Department of No.1 Hepatobiliary Pancreatic Surgery,The Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China;

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Keywords：

Recombinant human growth hormoneRatIschemic reperfusion injuryMalondialdehydeNuclear factor kappa BIntercellular adhesion molecule-1

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【Abstract】　Objective　To investigate the protective role of recombinant human growth hormone (rhGH )in ischemic reperfusion injury of rat liver and its mechanism. Methods　One hundred Male rats were randomly divided into two groups: the rhGH group and the control group. In the rhGH group, rhGH were injected （0.2U/100g weight） to rats seven days before the ischemic reperfusion injury, and in the control group, normal saline was injected instead. Serum levels of ALT， TNF-α and IL-1α were tested. Hepatic tissue was sectioned for to detect the level of EC and MDA, the expression of NF-κB and ICAM-1 mRNA on SEC. Ultrastructural characteristics histopathological characteristics were determined also. Results　Serum levels of ALT, TNF-α, IL-1α and the contents of MDA in the control group were significantly higher than those in the rhGH group （P＜0.05）. Comparied with control group, rhGH also decreased NF-κB activation, and reduced the expression of ICAM-1 mRNA of SEC in the liver cells （P＜0.05）. Electronic microscopic revealed that the hepatic sinusoidal endothelial cells and the hepatocellular mitochondria were injured in the control group. Pretreatment with the rhGH was able to significantly improved the pathological changes. Conclusion　rhGH might confer the protection to ischemic reperfusion injury of rat liver through reducing the expression of NF-κB to down-regulate cytokine (IL-1α,TNF-α), MDA and inhibition the expression of ICAM-1 mRNA.

Citation： RAN Jianghua,GUO Yongzhang,LI Li,ZHANG Shengning.. Protective Role of Recombinant Human Growth Hormone in Ischemic Reperfusion Injury of Rat Liver. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(5): 555-559. doi: Copy

1.	冉江华，张　捷，张家骅, 等. 重组人生长激素对肝叶切除术后肝功能改善和肝脏再生作用的动物实验和临床研究 [J]. 中国普外基础与临床杂志, 2001; 8(4)∶22.
2.	严律南, 曾　勇. 重组人生长激素对肝切除术后肝脏再生作用的临床研究 [J]. 中国实用外科杂志, 1999; 19(Suppl)∶21.
3.	戴朝六, 徐　锋, 田大治，等. 生长激素预处理保护大鼠肝脏缺血再灌注损伤的研究 [J]. 中国医科大学学报， 2005； 34（5）∶422.
4.	严律南主编. 现代肝移植学 [M]. 第1版. 北京：人民军医出版社， 2004∶1～8.
5.	龙建纲, 王学敏, 高宏翔，等. 丙二醛对大鼠肝线粒体呼吸功能及相关脱氢酶活性影响 [J]. 第二军医大学学报, 2005; 26(10)∶1131.
6.	张春芳，陈胜喜，郭海周，等. 缺血预处理对猪肺缺血再灌注性损伤的保护机制 [J]. 中南大学学报（医学版）， 2005; 30(1)∶64.
7.	Ferencz A, Racz B, Gasz B, et al. Intestinal ischemic preconditioning in rats and NF-kappaB activation [J].Microsurgery, 2006; 26(1)∶54.
8.	Suetsugu H, Iimuro Y, Uehara T, et al. Nuclear factor kappaB inactivation in the rat liver ameliorates short term total warm ischemia/reperfusion injury [J]. Gut, 2005; 54(6)∶835.

1. 　冉江华，张　捷，张家骅, 等. 重组人生长激素对肝叶切除术后肝功能改善和肝脏再生作用的动物实验和临床研究 [J]. 中国普外基础与临床杂志, 2001; 8(4)∶22.
2. 　严律南, 曾　勇. 重组人生长激素对肝切除术后肝脏再生作用的临床研究 [J]. 中国实用外科杂志, 1999; 19(Suppl)∶21.
3. 　戴朝六, 徐　锋, 田大治，等. 生长激素预处理保护大鼠肝脏缺血再灌注损伤的研究 [J]. 中国医科大学学报， 2005； 34（5）∶422.
4. 　严律南主编. 现代肝移植学 [M]. 第1版. 北京：人民军医出版社， 2004∶1～8.
5. 　龙建纲, 王学敏, 高宏翔，等. 丙二醛对大鼠肝线粒体呼吸功能及相关脱氢酶活性影响 [J]. 第二军医大学学报, 2005; 26(10)∶1131.
6. 　张春芳，陈胜喜，郭海周，等. 缺血预处理对猪肺缺血再灌注性损伤的保护机制 [J]. 中南大学学报（医学版）， 2005; 30(1)∶64.
7. 　Ferencz A, Racz B, Gasz B, et al. Intestinal ischemic preconditioning in rats and NF-kappaB activation [J].Microsurgery, 2006; 26(1)∶54.
8. 　Suetsugu H, Iimuro Y, Uehara T, et al. Nuclear factor kappaB inactivation in the rat liver ameliorates short term total warm ischemia/reperfusion injury [J]. Gut, 2005; 54(6)∶835.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Protective Role of Recombinant Human Growth Hormone in Ischemic Reperfusion Injury of Rat Liver

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