Researching the Significance on Expression of VEGF-C in Human Breast Carcinoma by Self-Constructed Tissue Chips_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIANG Yan ¹

Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China;

Corresponding author：

Keywords：

Breast carcinomaVascular endothelial growth factor-CTissue chipsLymph node metastasis

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【Abstract】　Objective　To research the significance on expression of vascular endothelial growth factor-C (VEGF-C) in human breast carcinoma, benign diseases and normal mammary gland by self-constructed tissue chips and research its relationship to regional lymph node metastasis. Methods　The tissue chips containing specimens of breast carcinoma, breast benign disease and normal mammary gland were designed and constructed. The expression of VEGF-C in the specimens was detected by the tissue chips and immunohistochemical method, and researched the relationship of the expression of VEGF-C in breast cancer with regional lymph node metastasis. Results　The positive rates of VEGF-C in the centre and borderline of carcinoma and distant mammary gland （the distance from the tumor’s bouncary ＞3 cm） were 69.4%（68 /98）, 69.1%（67 /97） and 52.9%（36 /68）, respectively， but not in benign disease and normal mammary gland specimens. The positive rates of VEGF-C in the centre and borderline of carcinoma in lymph node metastasis group 〔75.0%（51/68）， 76.1%(51/67)〕 were significantly higher than that of no metastasis group 〔25.0%（17/68），23.9%(16/67)〕, P＜0.05. The positive rates of VEGF-C in the centre and borderline of carcinoma and distant mammary gland were no correlation with size, type and clinical stage of tumor. Conclusion　The tissue chips is high efficiency and well quality control in multiple factor investigation. There are overexpression of VEGF-C in primary breast cancer, and that may play an important role in lymph node metastasis.

Citation： LIANG Yan,JIANG Jun,WANG Qingliang,BIAN Xiuwu.. Researching the Significance on Expression of VEGF-C in Human Breast Carcinoma by Self-Constructed Tissue Chips. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(5): 580-584. doi: Copy

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1. 　Kaipainen A, Korhonen J, Mustonen T, et al. Expression of the fms-like tyrosine kinase 4 gene becomes restricted to lymphatic endothelium during development [J]. Proc Natl Acad Sci USA， 1995; 92(8)∶3566.
2. 　Kononen J, Bubendorf L, Kallioniemi A, et al. Tissue microarrays for high-throughput molecular profiling of tumor specimens [J].Nat Med， 1998; 4(7)∶844.
3. 　卞修武，王清良. 高密度组织微阵列(组织芯片)技术及其在胶质瘤研究中的应用 [J]. 实用肿瘤杂志， 2004； 19(6)∶470.
4. 　Torhorst J, Bucher C, Kononen J, et al. Tissue microarrays for rapid linking of molecular changes to clinical endpoints [J].Am J Pathol， 2001; 159(6)∶2249.
5. 　Schraml P, Kononen J, Bubendorf L, et al. Tissue microarrays for gene amplification surveys in many different tumor types [J].Clin Cancer Res， 1999; 5(8)∶1966.
6. 　Joukov V, Pajusola K, Kaipainen A, et al. A novel vascular endothelial growth factor, VEGF-C, is a ligand for the Flt4 (VEGFR-3) and KDR (VEGFR-2) receptor tyrosine kinases [J]. EMBO J， 1996; 15(7)∶1751.
7. 　Wang JF, Ganju RK, Liu ZY, et al. Signal transduction in human hematopoietic cells by vascular endothelial growth factor related protein, a novel ligand for the FLT4 receptor [J].Blood， 1997; 90(9)∶3507.
8. 　Salven P, Lymboussaki A, Heikkila P, et al. Vascular endothelial growth factors VEGF-B and VEGF-C are expressed in human tumors [J]. Am J Pathol， 1998; 153(1)∶103.
9. 　Onogawa S, Kitadai Y, Tanaka S, et al. Expression of VEGF-C and VEGF-D at the invasive edge correlates with lymph node metastasis and prognosis of patients with colorectal carcinoma [J]. Cancer Sci， 2004; 95(1)∶32.
10. 　Skobe M, Hawighorst T, Jackson DG, et al. Induction of tumor lymphangiogenesis by VEGF-C promotes breast cancer metastasis [J]. Nat Med， 2001; 7(2)∶192.
11. 　Mattila MM, Ruohola JK, Karpanen T, et al. VEGF-C induced lymphangiogenesis is associated with lymph node metastasis in orthotopic MCF-7 tumors [J]. Int J Cancer， 2002; 98(6)∶946.
12. 　Kinoshita J, Kitamura K, Kabashima A, et al. Clinical significance of vascular endothelial growth factor-C (VEGF-C) in breast cancer [J]. Breast Cancer Res Treat， 2001; 66(2)∶159.
13. 　 Muller A, Homey B, Soto H, et al. Involvement of chemokine receptors in breast cancer metastasis [J].Nature， 2001; 410(6824)∶50.
14. 　Mashino K, Sadanaga N, Yamaguchi H, et al. Expression of chemokine receptor CCR7 is associated with lymph node metastasis of gastric carcinoma [J]. Cancer Res， 2002; 62(10)∶2937.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Researching the Significance on Expression of VEGF-C in Human Breast Carcinoma by Self-Constructed Tissue Chips

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