【Abstract】ObjectiveTo investigate the effect of ischemia-reperfusion （I/R） injury on apoptosis of pancreatic cells in rats with acute pancreatitis(AP).
MethodsFifty-four SD rats were randomized into 3 groups: pancreatitis group (n=24), I/R-injury group (n=24) and control group (n=6). The animal model of AP was induced by retrograde injection of 3% sodium taurocholate into biliopancreatic duct in rats. Pancreatic I/R was caused by blocking the inferior splenic artery and removing the clamp after AP induction. At 1 h, 3 h, 6 h and 12 h, groups of rats were sacrificed. A terminal deoxynucleotidyl transferase-mediated dUTP-biotion nick end labeling (TUNEL) was used to detect pancreatic apoptosis, and histological changes of the pancreas were observed.
ResultsPancreatic hemorrhage, necrosis were respectively observed in the pancreatitis rats at 6 h and the I/R-injury rats at 1 h. Histological changes of the pancreatitis rats at 1 h and 3 h were only congestion and edema. Apoptoic acinar cells increased after AP induction, the peak respectively appeared at 6 h in the pancreatitis rats and at 3 h in the I/R-injury rats. Compared with the pancreatitis rats, apoptosis index (AI) of the I/Rinjury rats was significantly higher at 1 h and 3 h (P＜0.01, P＜0.05, respectively), but lower at 6 h and 12 h (P＜0.05, P＜0.01, respectively).
ConclusionI/R injury can induce conversion of edematous pancreatitis to hemorrhagic necrotizing pancreatitis and apoptosis of acinar cells. Apoptosis may be a beneficial response to pancreatic injury in AP.
Citation：
CHANG Hua,YAN Jishen,WANG Pingyu,LIU Quncai.. Effect of Ischemia-Reperfusion Injury on Apoptosis of Pancreatic Cells in Rats with Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(2): 150-152. doi:
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- 1. Fujimoto K, Hosotani R, Wada M, et al. Ischemiareperfusion injury on the pancreas in rats: identification of acinar cell apoptosis [Ｊ]. J Surg Res, 1997; 71(2)127.
- 2. 常华，严际慎，罗建飞, 等. 大鼠急性胰腺炎胰腺细胞凋亡改变的实验研究 [Ｊ]. 肝胆胰外科杂志， 2001； 13(3)147.
- 3. Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholateinduced acute haemorrhagic pancreatitis [Ｊ]. Scand J Gastroenterol, 1980; 15(4)411.
- 4. Schmidt J, Lewandrowski K, Fernandezdel Castillo C, et al. Histopathologic correlates of serum amylase activity in acute experimental pancreatitis [Ｊ]. Dig Dis Sci, 1992; 37(9)1426.
- 5. 吕洪光, 邢军, 王智, 等.大鼠胰腺缺血/再灌注损伤的实验研究 [Ｊ]. 中国急救医学, 1999； 19(11)644.
- 6. Bassi D, Kollias N, Fernandezdel Castillo C, et al. Impairment of pancreatic microcirculation correlates with the severity of acute experimental pancreatitis [Ｊ]. J Am Coll Surg, 1994; 179(3)257.
- 7. 常华，严际慎，王平瑜, 等. 大鼠急性胰腺炎胰腺细胞凋亡与外周血浆TNFα的关系 [Ｊ]. 肝胆胰外科杂志, 2002； 14(4)215.
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