Effect of Nimesulide on DMBAInduced Mammary Tumors in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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GUO Guilong ¹ , ZHANG Xiaohua ¹ , YAO Zhenxiang. ²

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Keywords：

Nimesulide DMBA Mammary tumor Apoptosis

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【Abstract】ObjectiveTo observe the effect of nimesulide (NIM) on DMBA-induced mammary tumors and to investigate possible mechanisms of inhibiting tumors.
MethodsThe Wistar rats were randomly divided into four groups: DMBA group, NIM+DMBA group, NIM+diet group and diet group. The incidence and mean latent phase of mammary tumors were observed. The number and volume of tumors in every rat were measured. The apoptosis index and proliferation index were evaluated by TUNEL assay and PCNA immunohistochemical staining respectively.
Results The latent phases of mammary tumors in NIM+DMBA group were strikingly longer than those in DBMA group 〔(115±14.8) d vs (84±15.6) d,P＜0.01〕. The incidence of mammary tumors was 69.2% in DMBA group, 46.4% in NIM+DMBA group. There was obviously decreased incidence in NIM+DMBA group (P＜0.05）. The number and the mean volume of mammary tumors in every rat in NIM+DMBA group were markedly less than DBMA group 〔（0.82±0.31） vs （1.52±0.43），（3.24±1.21） cm3 vs （6.03±1.84） cm3，P＜0.05〕. The apoptosis index was obviously higher and the proliferation index was markedly lower in NIM+DMBA group compared with DMBA group〔(42.54±13.24)% vs (18.14±6.66)%, (20.25±4.97)% vs (36.36±5.02)%, P＜0.05〕.
ConclusionNIM can inhibit the development of DMBA-induced mammary tumors by inhibiting proliferation and inducing apoptosis.

Citation： GUO Guilong,ZHANG Xiaohua,YAO Zhenxiang.. Effect of Nimesulide on DMBAInduced Mammary Tumors in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(3): 219-222. doi: Copy

1.	Ristimaki A, Sivula A, Lundin J, et al. Prognostic significance of elevated cyclooxygenase2 expression in breast cancer [Ｊ]. Cancer Res， 2002; 62(3)∶ 632.
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6.	Rasso J, Gusterson BA, Rogers AE, et al. Comparative study of human and rat mammary tumorigenesis [Ｊ]. Lab Invest, 1990; 62(3)∶244.
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8.	Russo J, Russo IH. Experimentally induced mammary tumors in rats [Ｊ]. Breast Cancer Res Treat, 1996; 39(1)∶7.
9.	Harris RE, Chlebowski RT, Jackson RD, et al. Breast cancer and nonsteroidalantiinflammatory drugs: prospective results from the women’s health initiative [Ｊ]. Cancer Res, 2003; 63(15)∶6096.
10.	Egan KM, Stampfer MJ, Geovannrcci E, et al. Prospective study of regular aspirin use and the risk of breast cancer [Ｊ]. J Natl Cancer Inst, 1996; 88(14)∶988.
11.	Denda A, Kitayama W, Murata A, et al. Increased expression of cyclooxygenase2 protein during rat hepatocarcinogenesis caused by a cholinedeficient, Lamino aciddefined diet and chemopreventive efficacy of a specific inhibitor, nimesulide [Ｊ]. Carcinogenesis, 2002; 23(2)∶245.
12.	Tsujii M, DuBois RN. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase2 [Ｊ]. Cell, 1995; 83(4)∶493.
13.	Tardieu D, Jaeg JP, Deloly A, et al. The COX2 inhibitor nimesulide suppresses superoxide and 8hydroxydeoxyguanosine formation, and stimulates apoptosis in mucosa during early colonic inflammation in rats [Ｊ]. Carcinogenesis, 2000; 21(5)：973.

1. Ristimaki A, Sivula A, Lundin J, et al. Prognostic significance of elevated cyclooxygenase2 expression in breast cancer [Ｊ]. Cancer Res， 2002; 62(3)∶ 632.
2. 李建英，于皆平，罗和生，等. 选择性COX2抑制剂对胃癌细胞增殖的影响 [Ｊ]. 癌症, 2002; 21(6)∶625.
3. Tian G, Yu JP, Luo HS, et al. Effect of nimesulide on proliferation and apoptosis of human hepatoma SMMC7721 cells [Ｊ]. World J Gastroenterol, 2002; 8(3)∶483.
4. Nakatsugi S, Ohta T, Kawamori T, et al. Chemprevention by nimesulide, a selective cyclooxygenase2 inhibitor, of 2amin1methyl6phenylinidazo pyr I dineinduced mammary gland carcinogenesis in rats [Ｊ]. Jpn J Cancer Res, 2000; 91（9）∶886.
5. 姜军，陈意生，柳凤轩，等. 二甲基苯蒽诱导大鼠乳腺发生的组织形态学研究 [Ｊ]. 中国肿瘤临床,1998; 25（6）∶436.
6. Rasso J, Gusterson BA, Rogers AE, et al. Comparative study of human and rat mammary tumorigenesis [Ｊ]. Lab Invest, 1990; 62(3)∶244.
7. 邓敏，苏宁，朝月明,等. 三苯氧胺对二甲基苯蒽诱发的大鼠乳腺癌的影响 [Ｊ]. 苏州医学院学报, 1999; 19(9)∶961.
8. Russo J, Russo IH. Experimentally induced mammary tumors in rats [Ｊ]. Breast Cancer Res Treat, 1996; 39(1)∶7.
9. Harris RE, Chlebowski RT, Jackson RD, et al. Breast cancer and nonsteroidalantiinflammatory drugs: prospective results from the women’s health initiative [Ｊ]. Cancer Res, 2003; 63(15)∶6096.
10. Egan KM, Stampfer MJ, Geovannrcci E, et al. Prospective study of regular aspirin use and the risk of breast cancer [Ｊ]. J Natl Cancer Inst, 1996; 88(14)∶988.
11. Denda A, Kitayama W, Murata A, et al. Increased expression of cyclooxygenase2 protein during rat hepatocarcinogenesis caused by a cholinedeficient, Lamino aciddefined diet and chemopreventive efficacy of a specific inhibitor, nimesulide [Ｊ]. Carcinogenesis, 2002; 23(2)∶245.
12. Tsujii M, DuBois RN. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase2 [Ｊ]. Cell, 1995; 83(4)∶493.
13. Tardieu D, Jaeg JP, Deloly A, et al. The COX2 inhibitor nimesulide suppresses superoxide and 8hydroxydeoxyguanosine formation, and stimulates apoptosis in mucosa during early colonic inflammation in rats [Ｊ]. Carcinogenesis, 2000; 21(5)：973.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Nimesulide on DMBAInduced Mammary Tumors in Rats

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