Alteration of Fas/FasL mRNA Expression in Hepato carcinoma and Its Significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Haiming , DOU Kefeng , LI Ren , ZHOU Jingshi , DI Zhenyu , ZHANG Fuqin.

Department of Hepatobiliary Surgery， Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China;

Corresponding author：

Keywords：

Hepatocarcinoma Adjacent non-cancerous liver parenchyma Normal liver tissue Fas FasL mRNA

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【Abstract】ObjectiveTo measure the expressions of Fas/FasL mRNA in normal liver, adjacent non-cancerous liver parenchyma and hepatocarcinoma, and to explore the relationship between the expressions of Fas/FasL mRNA in those tissues and the hepatocellular carcinogenesis.
MethodsSemi-quantity reverse transcript-ploymerase chain reaction(QRTPCR) were performed to measure the relative quantity of the Fas and FasL mRNA expressions in normal liver (n=25), adjacent noncancerous liver parenchyma(n=40) and hepatocarcinoma(n=40).
ResultsThe relative quantity of Fas and FasL mRNA expressed in normal liver, adjacent non-cancerous liver parenchyma and hepatocarcinoma were 0.792±0.039 vs 0.245±0.043，0.857±0.031 vs 0.429±0.035 and 0.473±0.047 vs 0.185±0.041, respectively. The relative quantity of Fas mRNA expression in hepatocarcinoma was lower than that of normal liver tissue and adjacent non-cancerous liver parenchyrna (P＜0.05). The relative quantity of FasL mRNA expression in hepatocarcinoma was also lower than that of normal liver tissue (P＜0.05) and adjacent non-cancerous liver parenchyma (P＜0.01), but its expression in adjacent non-cancerous liver parenchyma was higher than that of normal liver tissue (P＜0.05).
ConclusionHepatorcarcinoma may escape the immune surveillance of the host, not only by means of reducing Fas expression, but also through adjacent non-cancerous liver parenchyma’s increasing expression of FasL to induce apoptosis of contact lymphocyte which highly expresses Fas.

Citation： LI Haiming,DOU Kefeng,LI Ren,ZHOU Jingshi,DI Zhenyu,ZHANG Fuqin.. Alteration of Fas/FasL mRNA Expression in Hepato carcinoma and Its Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(3): 284-286. doi: Copy

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9.	Roskams T, Libbrecht L, Van Damme B, et al. Fas and Fas ligand: strong coexpression in human hepatocytes surrounding hepatocellular carcinoma; can cancer induce suicide in peritumoural cells? [Ｊ]. J Pathol, 2000; 191(2)∶150.
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11.	梁云,陆斌,李晓冬, 等.肝癌细胞通过Fas/FasL途径触发T淋巴细胞的凋亡 [Ｊ]. 第二军医大学学报, 2002; 22(11)∶1021.

1. Shima Y, Nakao K, Nakashima T, et al. Activation of caspase8 in transforming growth factorbetainduced apoptosis of human hepatoma cells [Ｊ]. Hepatology, 1999; 30(5)∶ 1215.
2. Suzuki A, Hayashida M, Kawano H, et al. Hepatocyte growth factor promotes cell survival from fasmediated cell death in hepatocellular carcinoma cells via Akt activation and Fasdeathinducing signaling complex suppression [Ｊ]. Hepatology, 2000; 32(4 Pt 1)∶796.
3. Ito Y, Monden M, Takeda T, et al. The status of Fas and Fas ligand expression can predict recurrence of hepatocellular carcinoma [Ｊ]. Br J Cancer, 2000; 82(6)∶1211.
4. 张俊峰,石学涛,衣龙海, 等.Fas/FasL在肝癌免疫逃避中作用的探讨 [Ｊ]. 山东医药•内科(上半月),2002; 42(16)∶1.
5. Higaki K, Yano H, Kojiro M. Fas antigen expression and its relationship with apoptosis in human hepatocellular carcinoma and noncancerous tissues [Ｊ]. Am J Pathol, 1996; 149(2)∶429.
6. You KR, Shin MN, Park RK, et al. Activation of caspase8 during N(4hydroxyphenyl)retinamideinduced apoptosis in Fasdefective hepatoma cells [Ｊ]. Hepatology, 2001; 34(6)∶1119.
7. 梁云,陆斌,王华菁, 等. p53基因、Fas/FasL在提高肝癌细胞化疗药物敏感性中的作用 [Ｊ]. 中华实验外科杂志, 2002; 19(4)∶342.
8. Srand S, Hofmann WJ, Hug H, et al. Lymphcyte apoptosis induce by CD95(APO1/Fas) Ligandexpressing tumor immune escape [Ｊ]. Science, 1996; 274(4)∶1363.
9. Roskams T, Libbrecht L, Van Damme B, et al. Fas and Fas ligand: strong coexpression in human hepatocytes surrounding hepatocellular carcinoma; can cancer induce suicide in peritumoural cells? [Ｊ]. J Pathol, 2000; 191(2)∶150.
10. Nagao M, Nakajima Y, Hisanaga M, et al. The alteration of Fas receptor and ligand system in hepatocellular carcinomas: how do hepatoma cells escape from the host immune surveillance in vivo? [Ｊ]. Hepatology, 1999; 30(2)∶413.
11. 梁云,陆斌,李晓冬, 等.肝癌细胞通过Fas/FasL途径触发T淋巴细胞的凋亡 [Ｊ]. 第二军医大学学报, 2002; 22(11)∶1021.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Alteration of Fas/FasL mRNA Expression in Hepato carcinoma and Its Significance

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