【Abstract】Objective To study the regulatory ability of peroxisome proliferatoractivated receptor γ(PPARγ) ligands to the inflammatory response in human gallbladder epithelial cells.
Methods Culture human gallbladder epithelial cells and identify them . Cells were treated for 24 hours with 0, 10 μmol/L, 20 μmol/L, 30 μmol/L, 50 μmol/L and 100 μmol/L of Ciglitazone during cellular growth peak(5th day), then stimulated them with hIL-1β 5 ng/ml for 2 hours and measured the concentration of IL-6、IL-8 and TNF-α in cellular supernatants by riadioimmunoassay.
Results Contrasted with control group, the expression of IL-6 and IL-8 in each test group were inhibited （P＜0.001）. The IL-6 and IL-8 levels were gradually dropped and corelated with the dosage of Cigtitazone, and manifested dosagedependence (P＜0.001). The concentration of TNF-α could not be measured.
Conclusion PPARγ ligands can inhibit the expression of IL-6 and IL-8 in human gallbladder epithelial cells and probably produce effect in the regulation of cholecystic inflammation.
Citation：
LIU Jiangwen,CHENG Nansheng,PAN Guangdong,XIONG Xianze,YAN Lan.. Experimental Study on PPARγ Ligands Regulating the Inflammatory Cytokines in Human Gallbladder Epithelial Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(4): 336-339. doi:
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- 1. Martin C, Saux P, Mege JL, et al. Prognostic values of serum cytokines in septic shock [Ｊ]. Intensive Care Med, 1994; 20(4)∶272.
- 2. Delerive P, De Bosscher K, Besnard S, et al. Peroxisome proliferatoractivated receptor alpha negatively regulates the vascular inflammatory gene response by negative crosstalk with transcription factors NFkappaB and AP1 [Ｊ]. J Biol Chem, 1999; 274(45)∶32048.
- 3. Ricote M, Li AC, Willson TM, et al. The peroxisome proliferatoractivated receptorgamma is a negative regulator of macrophage activation [Ｊ]. Nature, 1998; 391(6662)∶79.
- 4. Jiang C, Ting AT, Seed B. PPARgamma agonists inhibit production of monocyte inflammatory cytokines [Ｊ]. Nature, 1998; 391(6662)∶82.
- 5. Su CG, Wen X, Bailey ST, et al. A novel therapy for colitis utilizing PPARgamma ligands to inhibit the epithelial inflammatory response [Ｊ]. J Clin Invest, 1999; 104(4)∶383.
- 6. Yamamoto Y, Gaynor RB. Therapeutic potential of inhibition of the NFkappaB pathway in the treatment of inflammation and cancer [Ｊ]. J Clin Invest, 2001; 107(2)∶135.
- 7. Goebeler M, Gillitzer R, Kilian K, et al. Multiple signaling pathways regulate NFkappaBdependent transcription of the monocyte chemoattractant protein1 gene in primary endothelial cells [Ｊ]. Blood, 2001; 97(1)∶46.
- 8. Chung SW, Kang BY, Kim SH, et al. Oxidized low density lipoprotein inhibits interleukin12 production in lipopolysaccharideactivated mouse macrophages via direct interactions between peroxisome proliferatoractivated receptorgamma and nuclear factorkappa B [Ｊ]. J Biol Chem, 2000; 275(42)∶32681.
