Experimental Study on PPARγ Ligands Regulating the Inflammatory Cytokines in Human Gallbladder Epithelial Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Jiangwen , CHENG Nansheng , PAN Guangdong , XIONG Xianze , YAN Lan.

Corresponding author：

Keywords：

Peroxisome proliferator-activated receptor γ Ligand Gallbladder epithelial cell Interleukin Cholecystitis

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

【Abstract】Objective To study the regulatory ability of peroxisome proliferatoractivated receptor γ(PPARγ) ligands to the inflammatory response in human gallbladder epithelial cells.
Methods Culture human gallbladder epithelial cells and identify them . Cells were treated for 24 hours with 0, 10 μmol/L, 20 μmol/L, 30 μmol/L, 50 μmol/L and 100 μmol/L of Ciglitazone during cellular growth peak(5th day), then stimulated them with hIL-1β 5 ng/ml for 2 hours and measured the concentration of IL-6、IL-8 and TNF-α in cellular supernatants by riadioimmunoassay.
Results Contrasted with control group, the expression of IL-6 and IL-8 in each test group were inhibited （P＜0.001）. The IL-6 and IL-8 levels were gradually dropped and corelated with the dosage of Cigtitazone, and manifested dosagedependence (P＜0.001). The concentration of TNF-α could not be measured.
Conclusion PPARγ ligands can inhibit the expression of IL-6 and IL-8 in human gallbladder epithelial cells and probably produce effect in the regulation of cholecystic inflammation.

Citation： LIU Jiangwen,CHENG Nansheng,PAN Guangdong,XIONG Xianze,YAN Lan.. Experimental Study on PPARγ Ligands Regulating the Inflammatory Cytokines in Human Gallbladder Epithelial Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(4): 336-339. doi: Copy

1.	Martin C, Saux P, Mege JL, et al. Prognostic values of serum cytokines in septic shock [Ｊ]. Intensive Care Med, 1994; 20(4)∶272.
2.	Delerive P, De Bosscher K, Besnard S, et al. Peroxisome proliferatoractivated receptor alpha negatively regulates the vascular inflammatory gene response by negative crosstalk with transcription factors NFkappaB and AP1 [Ｊ]. J Biol Chem, 1999; 274(45)∶32048.
3.	Ricote M, Li AC, Willson TM, et al. The peroxisome proliferatoractivated receptorgamma is a negative regulator of macrophage activation [Ｊ]. Nature, 1998; 391(6662)∶79.
4.	Jiang C, Ting AT, Seed B. PPARgamma agonists inhibit production of monocyte inflammatory cytokines [Ｊ]. Nature, 1998; 391(6662)∶82.
5.	Su CG, Wen X, Bailey ST, et al. A novel therapy for colitis utilizing PPARgamma ligands to inhibit the epithelial inflammatory response [Ｊ]. J Clin Invest, 1999; 104(4)∶383.
6.	Yamamoto Y, Gaynor RB. Therapeutic potential of inhibition of the NFkappaB pathway in the treatment of inflammation and cancer [Ｊ]. J Clin Invest, 2001; 107(2)∶135.
7.	Goebeler M, Gillitzer R, Kilian K, et al. Multiple signaling pathways regulate NFkappaBdependent transcription of the monocyte chemoattractant protein1 gene in primary endothelial cells [Ｊ]. Blood, 2001; 97(1)∶46.
8.	Chung SW, Kang BY, Kim SH, et al. Oxidized low density lipoprotein inhibits interleukin12 production in lipopolysaccharideactivated mouse macrophages via direct interactions between peroxisome proliferatoractivated receptorgamma and nuclear factorkappa B [Ｊ]. J Biol Chem, 2000; 275(42)∶32681.

1. Martin C, Saux P, Mege JL, et al. Prognostic values of serum cytokines in septic shock [Ｊ]. Intensive Care Med, 1994; 20(4)∶272.
2. Delerive P, De Bosscher K, Besnard S, et al. Peroxisome proliferatoractivated receptor alpha negatively regulates the vascular inflammatory gene response by negative crosstalk with transcription factors NFkappaB and AP1 [Ｊ]. J Biol Chem, 1999; 274(45)∶32048.
3. Ricote M, Li AC, Willson TM, et al. The peroxisome proliferatoractivated receptorgamma is a negative regulator of macrophage activation [Ｊ]. Nature, 1998; 391(6662)∶79.
4. Jiang C, Ting AT, Seed B. PPARgamma agonists inhibit production of monocyte inflammatory cytokines [Ｊ]. Nature, 1998; 391(6662)∶82.
5. Su CG, Wen X, Bailey ST, et al. A novel therapy for colitis utilizing PPARgamma ligands to inhibit the epithelial inflammatory response [Ｊ]. J Clin Invest, 1999; 104(4)∶383.
6. Yamamoto Y, Gaynor RB. Therapeutic potential of inhibition of the NFkappaB pathway in the treatment of inflammation and cancer [Ｊ]. J Clin Invest, 2001; 107(2)∶135.
7. Goebeler M, Gillitzer R, Kilian K, et al. Multiple signaling pathways regulate NFkappaBdependent transcription of the monocyte chemoattractant protein1 gene in primary endothelial cells [Ｊ]. Blood, 2001; 97(1)∶46.
8. Chung SW, Kang BY, Kim SH, et al. Oxidized low density lipoprotein inhibits interleukin12 production in lipopolysaccharideactivated mouse macrophages via direct interactions between peroxisome proliferatoractivated receptorgamma and nuclear factorkappa B [Ｊ]. J Biol Chem, 2000; 275(42)∶32681.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study on PPARγ Ligands Regulating the Inflammatory Cytokines in Human Gallbladder Epithelial Cells

Abstract Full text Figures/Tables Video References Cited by

Format

Content