Study on the Relationship Between STHZMDR1 Gene Expression in Liver Cell and Cholesterol Calculus in Gallbladder_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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LIU Jun ¹ , LONG Houyong ¹ , MING Hanxin ¹ , AN Chuanguo ² , QU Xun. ³

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Keywords：

Polymorase chain reaction MDR1 gene Cholesterol calculus in gallbladder

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【Abstract】Objective To explore the relationship between the expression of MDR1 gene in liver cell and the formation of cholesterol calculus in gallbladder.
Methods The mRNA expression level of MDR1 gene in liver cell of the cholesterol calculus group and the normal control group were measured through reverse transcriptionpolymerase chain reaction (RT-PCR)， and microglobulin β2 was used as internal contrast.
Results The MDR1 mRNA expression level of the cholesterol calculus group was lower than that of the normal control group(1.30±0.19 vs 2.25±0.28, P＜0.01).
Conclusion The formation of cholesterol calculus in gallbladder is related to the reducd expression level of MDR1 gene in liver cell.

Citation： LIU Jun,LONG Houyong,MING Hanxin,AN Chuanguo,QU Xun.. Study on the Relationship Between STHZMDR1 Gene Expression in Liver Cell and Cholesterol Calculus in Gallbladder. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(4): 346-348. doi: Copy

1.	Luker GD, Dahlheimer JL, Ostlund RE Jr, et al. Decreased hepatic accumulation and enhanced esterification of cholesterol in mice deficient in mdr1a and mdr1b Pglycoproteins [Ｊ]. J Lipid Res， 2001； 42(9)∶1389.
2.	Wang E, Casciano CN, Clement RP, et al. Cholesterol interaction with the daunorubicin binding site of Pglycoprotein [Ｊ]. Biochem Biophys Res Commun, 2000； 276(3)∶909.
3.	Luker GD, Nilsson KR, Covey DF, et al. Multidrug resistance (MDR1) Pglycoprotein enhances esterification of plasma membrane cholesterol [Ｊ]. J Biol Chem, 1999； 274(11)∶6979.
4.	Lee JM, Trauner M, Soroka CJ, et al. Expression of the bile salt export pump is maintained after chronic cholestasis in the rat [Ｊ]. Gastroenterology, 2000； 118(1)∶163.
5.	Abulrob AG, Gumbleton M. Transport of phosphatidylcholine in MDRnegative epithelial cell lines via druginduced MDR1 Pglycoprotein [Ｊ]. Biochem Biophys Res Commun, 1999； 262(1)∶121.
6.	Hooiveld GJ, van Montfoort JE, Meijer DK, et al. Function and regulation of ATPbinding cassette transport proteins involved in hepatobiliary transport [Ｊ]. Eur J Pharm Sci, 2001； 12(4)∶525.
7.	Konikoff FM. Gallstonesapproach to medical management [Ｊ]. Med Gen Med, 2003； 5(4)∶8.

1. Luker GD, Dahlheimer JL, Ostlund RE Jr, et al. Decreased hepatic accumulation and enhanced esterification of cholesterol in mice deficient in mdr1a and mdr1b Pglycoproteins [Ｊ]. J Lipid Res， 2001； 42(9)∶1389.
2. Wang E, Casciano CN, Clement RP, et al. Cholesterol interaction with the daunorubicin binding site of Pglycoprotein [Ｊ]. Biochem Biophys Res Commun, 2000； 276(3)∶909.
3. Luker GD, Nilsson KR, Covey DF, et al. Multidrug resistance (MDR1) Pglycoprotein enhances esterification of plasma membrane cholesterol [Ｊ]. J Biol Chem, 1999； 274(11)∶6979.
4. Lee JM, Trauner M, Soroka CJ, et al. Expression of the bile salt export pump is maintained after chronic cholestasis in the rat [Ｊ]. Gastroenterology, 2000； 118(1)∶163.
5. Abulrob AG, Gumbleton M. Transport of phosphatidylcholine in MDRnegative epithelial cell lines via druginduced MDR1 Pglycoprotein [Ｊ]. Biochem Biophys Res Commun, 1999； 262(1)∶121.
6. Hooiveld GJ, van Montfoort JE, Meijer DK, et al. Function and regulation of ATPbinding cassette transport proteins involved in hepatobiliary transport [Ｊ]. Eur J Pharm Sci, 2001； 12(4)∶525.
7. Konikoff FM. Gallstonesapproach to medical management [Ｊ]. Med Gen Med, 2003； 5(4)∶8.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on the Relationship Between STHZMDR1 Gene Expression in Liver Cell and Cholesterol Calculus in Gallbladder

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