【Abstract】Objective To establish animal model of orthotopic liver transplantation(OLT) in miniature pigs with high standardization, reproducibility and stability.
Methods OLTs were performed without venovenous bypass in Bama miniature pigs. The survival rates and the changes of hemodynamics and metabolism were investigated.
Results Twenty OLTs were performed between pairs of miniature pigs. The mean operative time and anhepatic phase were (181±25.8) min and (28.4±3.2) min respectively. During the anhepatic phase, dramatic hemodynamics and metabolism changes accompanied hyperkalemia identified. MAP and CVP decreased from (14.59±1.68) kPa and (0.66±0.11) kPa to (5.87±0.91) kPa and (0.27±0.10) kPa respectively, while temperature, pH, BE and HCO3- were significantly reduced (P＜0.05) and HR and K+ in serum were remarkable increased. After reperfusion, the disorder of hemodynamics and metabolism described above recovered gradually. 1week survival rate was 90%. Sixteen animals survived more than 2 weeks. AST, ALT and TBIL were significantly increased and reached the peak level on postoperative 1 day. From postoperative 2 day, AST, ALT and TBIL began to decrease and reached postanaesthesia level on postoperative 7 day.
Conclusion The animal model of OLT without venovenous bypass in miniature pig, with its high standardization, reproducibility and stability, is an ideal one for series studies related to liver transplantation.
Citation：
ZHENG Shuguo,FENG Xiaobin,DONG Jiahong,LENG J ianjun,LI Kun,ZHENG Ping,XIONG Yan,ZHANG Yujun.. Establishment and Evaluation of Orthotopic Liver Transplantation Without VenoVenous Bypass in Miniature Pigs. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(5): 451-454. doi:
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- 2. 何春水，何延政，钟武，等. 猪解剖生理特点对猪原位肝移植的影响 [Ｊ]. 中国普通外科杂志， 2002; 11（1）∶41.
- 3. Laino GM, Anastasi A, Fabbri LP, et al. Experimental liver transplantation in pigs. Surgical technique and complications [Ｊ]. Minerva Chir, 1996; 51(10)∶765.
- 4. Oike F, Uryuhara K, Otsuka M, et al. Simplified technique of orthotopic liver transplantation in pigs [Ｊ]. Transplantation, 2001; 71(2)∶328.
- 5. Oldhafer KJ, Hauss J, Gubernatis G, et al. Liver transplantation in pigs: a model for studying reperfusion injury [Ｊ]. J Invest Surg, 1993; 6(5)∶439.
- 6. Monden M, Barter RH, Fortner JG, et al. A simple method of orthotopic liver transplantation in dogs [Ｊ]. Ann Surg, 1982; 195(1)∶110.
- 7. Tanaka K, Nishimura A, Ogata S, et al. A simple method for orthotopic liver transplantation in pigs——a new cuff technique using expanded polytetrafluoroethylene vascular grafts for portal and vena caval anastomosis [Ｊ]. Transplantation, 1994; 58(10)∶1139.
- 8. Fukumoto T, Ku Y, Tominaga M, et al. A novel paper cuff for vascular reconstruction in canine liver transplantation [Ｊ]. Surg Today, 1996; 26(4)∶295.
