Human Interleukin-13 Affects Expression of Adhesive Molecules on Bovine Aortic Endothelial Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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CHEN Gang ¹ , FU Zhiren ¹ , WANG Lianghua ² , JIAO Binghua. ²

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Keywords：

Human interlukin-13 Bovine aortic endothelial cell Intercellular adhesion molecule-1 E-selectin

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【Abstract】Objective To investigate the effects of human interlukin-13 (hIL-13) on the expression of E-selectin and intercellular adhesion molecule-1(ICAM-1) on bovine aortic endothelial cells(BAECs) stimulated by tumor necrosis factor alpha(TNF-α), and to provide experimental basis for hIL-13 inducing immunity endure and relieving the repulsion reaction of xenograft.
Methods BAECs were co-cultured with different concentrations of hIL-13 for 2 h and followed by co-cultured with 4 ng/ml TNF-α for 6 h or 18 h. The expressions of E-selectin and ICAM-1 on BAECs were detected by Cell-ELISA. The effect of hIL-13 on activity of BAECs was detected by MTT colorimetry.
Results BAECs pretreateded with hIL-13 could inhibit the expression of E-selectin and ICAM-1 induced by TNF-α, and showed a doesdependent manner from 5 ng/ml to 20 ng/ml of hIL-13 (P＜0.01). The experimental result of BAECs activity measured by MTT proved no significant difference in the activities of BAECs in every experimental groups compared with control group’s.
Conclusion hIL-13 could inhibit the expression of E-selectin and ICAM-1 on BAECs induced by TNF-α, which may contribute to the xenotransplant immune tolerance.

Citation： CHEN Gang,FU Zhiren,WANG Lianghua,JIAO Binghua.. Human Interleukin-13 Affects Expression of Adhesive Molecules on Bovine Aortic Endothelial Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(5): 469-472. doi: Copy

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6.	Satoh S, Thomson AW, Nakamura K, et al. Circulating ICAM1, Eselectin, IL2 receptor, and HLA class I in human small bowel, liver, and small bowelplusliver transplant recipients [Ｊ]. Transplantation, 1995; 60(6)∶558.
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8.	Burke GW, Ciancio G, Cirocco R, et al. Association of interleukin10 with rejectionsparing effect in septic kidney transplant recipients [Ｊ]. Transplantation, 1996; 61(7)∶1114.
9.	Ishido N, Matsuoka J, Matsuno T, et al. Induction of donorspecific hyporesponsiveness and prolongation of cardiac allograft survival by jejunal administration of donor splenocytes [Ｊ]. Transplantation, 1999; 68(9)∶1377.
10.	Chen CC, Rosenbloom CL, Anderson DC, et al. Selective inhibition of Eselectin, vascular cell adhesion molecule1, and intercellular adhesion molecule1 expression by inhibitors of I kappa Balpha phosphorylation [Ｊ]. J Immunol, 1995; 155(7)∶3538.
11.	Montgomery KF, Osborn L, Hession C, et al. Activation of endothelialleukocyte adhesion molecule 1 (ELAM1) gene transcription [Ｊ]. Proc Natl Acad Sci USA, 1991; 88(15)∶6523.
12.	Hou J, Baichwal V, Cao Z. Regulatory elements and transcription factors controlling basal and cytokineinduced expression of the gene encoding intercellular adhesion molecule 1 [Ｊ]. Proc Natl Acad Sci USA, 1994； 91(24)∶11641.
13.	Martin PL, Davis D, Weiss M, et al. Xenotransplantation of bovine bone marrow stromal cells into pig fetuses: incorporation into skeletal muscle [Ｊ]. Anim Biotechnol, 2001; 12(2)∶183.
14.	Yoo JG, Choe SY, Rho GJ. Efficient production of cloned bovine embryos using cdc2 kinase inhibitor [Ｊ]. Reprod Domest Anim, 2003; 38(6)∶444.
15.	Lentsch AB, Shanley TP, Sarma V, et al. In vivo suppression of NFkappa B and preservation of I kappa B alpha by interleukin10 and interleukin13 [Ｊ]. J Clin Invest, 1997; 100(10)∶2443.

1. Macarak EJ, Howard BV, Kefalides NA. Properties of calf endothelial cells in culture [Ｊ]. Lab Invest， 1977; 36(1)∶62.
2. Minter AJ, Keoshkerian E, Chesterman CN, et al. Fibroblast growth factor and heparin protect endothelial cells from the effects of interleukin 1 [Ｊ]. J Cell Physiol, 1996; 167(2)∶229.
3. Villarete LH, Remick DG. Nitric oxide regulation of IL8 expression in human endothelial cells [Ｊ]. Biochem Biophys Res Commun, 1995; 211(2)∶671.
4. Weigel G, Grimm M, Griesmacher A, et al. Adhesion molecule behavior during rejection and infection episodes after heart transplantation [Ｊ]. Clin Chem Lab Med, 2000; 38(5)∶403.
5. Yamazaki S, Isobe M, Suzuki J, et al. Role of selectindependent adhesion in cardiac allograft rejection [Ｊ]. J Heart Lung Transplant, 1998; 17(10)∶1007.
6. Satoh S, Thomson AW, Nakamura K, et al. Circulating ICAM1, Eselectin, IL2 receptor, and HLA class I in human small bowel, liver, and small bowelplusliver transplant recipients [Ｊ]. Transplantation, 1995; 60(6)∶558.
7. Nowaczyk M, Gorski A, Durlik M, et al. Role of adhesion molecules in chronic allograft rejection [Ｊ]. Arch Immunol Ther Exp (Warsz), 1999; 47(6)∶373.
8. Burke GW, Ciancio G, Cirocco R, et al. Association of interleukin10 with rejectionsparing effect in septic kidney transplant recipients [Ｊ]. Transplantation, 1996; 61(7)∶1114.
9. Ishido N, Matsuoka J, Matsuno T, et al. Induction of donorspecific hyporesponsiveness and prolongation of cardiac allograft survival by jejunal administration of donor splenocytes [Ｊ]. Transplantation, 1999; 68(9)∶1377.
10. Chen CC, Rosenbloom CL, Anderson DC, et al. Selective inhibition of Eselectin, vascular cell adhesion molecule1, and intercellular adhesion molecule1 expression by inhibitors of I kappa Balpha phosphorylation [Ｊ]. J Immunol, 1995; 155(7)∶3538.
11. Montgomery KF, Osborn L, Hession C, et al. Activation of endothelialleukocyte adhesion molecule 1 (ELAM1) gene transcription [Ｊ]. Proc Natl Acad Sci USA, 1991; 88(15)∶6523.
12. Hou J, Baichwal V, Cao Z. Regulatory elements and transcription factors controlling basal and cytokineinduced expression of the gene encoding intercellular adhesion molecule 1 [Ｊ]. Proc Natl Acad Sci USA, 1994； 91(24)∶11641.
13. Martin PL, Davis D, Weiss M, et al. Xenotransplantation of bovine bone marrow stromal cells into pig fetuses: incorporation into skeletal muscle [Ｊ]. Anim Biotechnol, 2001; 12(2)∶183.
14. Yoo JG, Choe SY, Rho GJ. Efficient production of cloned bovine embryos using cdc2 kinase inhibitor [Ｊ]. Reprod Domest Anim, 2003; 38(6)∶444.
15. Lentsch AB, Shanley TP, Sarma V, et al. In vivo suppression of NFkappa B and preservation of I kappa B alpha by interleukin10 and interleukin13 [Ｊ]. J Clin Invest, 1997; 100(10)∶2443.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Human Interleukin-13 Affects Expression of Adhesive Molecules on Bovine Aortic Endothelial Cells

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