【Abstract】Objective The injury induced by hepatic artery ischemia (HAI) in the liver transplantation procedure and the protective effects of using hepatic artery bridge-conduit (HABC) technique were studied.
Methods Thirtytwo dogs were randomly divided into 4 groups: control, HAI 30 min, HAI 2 h and HABC groups. We observed the pathological changes of hepatocytes and biliary tract tissues and the microstructure of chondriosome, which were based on the model of auto-orthotopic liver transplantation in dogs. Biochemical and spectrophotometric methodswere used to evaluate the content of MDA and SOD, SDH activities in the graft liver tissue respectively.
Results The pathologic and electrical microscopic changes of hepatocytes and epithelial cells of bile ducts were found in HAI 30 min and HAI 2 h groups,while the content of MDA increased to （1.652±0.222） nmol/mg prot and (2.379±0.526) nmol/mg prot, and SOD activity decreased to (11.15±3.9) U/mg prot and (9.47±3.4) U/mg prot. At the same time, SDH activity was also down-regulated to 0.362±0.019 and 0.281±0.029. Compared with control group, the differences were significant (P lt;0.05, P lt;0.01). But these changes of functional index caused by HAI injury were not significant in HABC group.
Conclusion The HABC technique can not only avoid HAI injury during operation but also alleviate the occurrence of complication after transplantation, especially the biliary tract complication.
Citation：
LI Yiming,XU Jinkai,JI Hong,XU Xin,HOU Chongzhi.. Protection from Hepatic Artery Ischemia with Hepatic Artery BridgeConduit in Liver Transplantation. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(5): 473-476下转479. doi:
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- 1. 黎一鸣，秦兆寅，康亚安，等. 简易犬自体肝原位移植模型建立及意义 [Ｊ]. 中华实验外科杂志, 1995; 12(2)∶113.
- 2. Li Yiming, Jenkins RL, Lewis WD. The primary observation and approach to effects of ischemic time of hepatic artery on the results of liver transplantation [Ｊ]. J XI’AN MED UNIV, 1993; 5(2)∶81.
- 3. Margarit C, Hidalgo E, Lazaro JL, et al. Biliary complications secondary to late hepatic artery thrombosis in adult liver transplant patients [Ｊ]. Transpl Int, 1998; 11(Suppl 1)∶S251.
- 4. Massarollo PC, Mies S, Raia S. Simultaneous arterial and portal revascularization in liver transplantation [Ｊ]. Transplant Proc, 1998; 30(6)∶2883.
- 5. Schleimer K, Stippel DL, Kasper HU, et al. Improved microcirculation of a liver graft by controlled portal vein arterializa（下转第479页） （上承第476页）tion [Ｊ]. J Surg Res, 2004; 116(2)∶202.
- 6. van As AB, Lotz Z, Tyler M, et al. Reperfusion injury associated with portal venous and hepatic arterial perfusion in liver transplantation [Ｊ]. Transplantation, 2002; 74(2)∶158.
- 7. Miyagawa Y, Imamura H, Soeda J, et al. Fate of hepatocyte and sinusoidal lining cell function and kinetics after extended cold preservation and transplantation of the rat liver [Ｊ]. Liver Transpl, 2002; 8(4)∶370.
- 8. Ardizzone G, Andorno E, Demartini M, et al. Portal vein pressure and graft oxygen consumption monitoring during liver transplantation [Ｊ]. Transplant Proc, 2003; 35(8)∶3015.
- 9. 陈主初主编. 病理生理学 [M]. 第1版. 北京： 人民卫生出版社，2001∶92.
- 10. Kojima N, Sato M, Suzuki A, et al. Enhanced expression of B71, B72, and intercellular adhesion molecule 1 in sinusoidal endothelial cells by warm ischemia/reperfusion injury in rat liver [Ｊ]. Hepatology, 2001; 34(4 Pt 1)∶751.
- 11. 黎一鸣, 秦兆寅, 康亚安, 等. 肝动脉“桥式”置管法对供肝动脉缺血损伤保护作用的实验研究 [Ｊ]. 中华实验外科杂志, 1996; 13(增刊)∶129.
