EFFECTS AND CLINICAL SIGNIFICANCE OF VINCRISTINE IN GASTRIN-STIMULATING CELL PROLIFERATION ON HUMAN COLONIC CANCER CELL LINE SW480_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XIE Bin , HE Suangwu , WANG Daike.

Department of General Surgery, Research Institute of Field Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042;

Corresponding author：

Keywords：

Vincristine Gastrin Colon cancer Cell line

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Objective To investigate the mechanism and clinical significance of vincristine (VCR) inhibiting gastrinproliferation effects on human colon cell line SW480.
Methods Effects of VCR on the viable cell count (A value), myoinositol triphosphate (IP3, CPM value), 〔Ca2+〕i and protein kinase C (PKC) activity of human colon cell line SW480 were evaluated in vitro by MTT assay,3Hmyoinositol incorporation, fluorescence measurements and γ-32P-ATP incorporation.
Results A value of VCR+PG group was lower than that of PG or control group (Ｐ＜0.01 vs control, Ｐ＜0.01 vs PG). The concentration of IP3 or 〔Ca2+〕i in VCR+PG group was lower than that in PG group (Ｐ＜0.01 vs PG); and the PKC activity of membrane was lower than that in PG group (Ｐ＜0.05 vs PG, Ｐ＞0.05 vs control). Conclusion Effects of vincristine may be through the phosphoinositide signaling pathway on gastrinstimulating cell proliferation in human colon cell line SW480. It has provided an experimental evidence for antisignaling therapy for patients with colon cancer.

Citation： XIE Bin,HE Suangwu,WANG Daike.. EFFECTS AND CLINICAL SIGNIFICANCE OF VINCRISTINE IN GASTRIN-STIMULATING CELL PROLIFERATION ON HUMAN COLONIC CANCER CELL LINE SW480. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(1): 3-5. doi: Copy

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1. Yassin RR, Murthy SNS. Possible involvement of protein kinase C in mediating gastrininduced response in rat colonic epithelium 〔Ｊ〕. Peptides, 1991; 12(5)∶925.
2. Ishizuka J, Beauchamp RD, Townsend CM, et al. Receptormediated autocrine growthstimulatory effect of 5hydroxytryptamine on cultured human pancreatic carcinoid cells 〔Ｊ〕. J Cell Physiol, 1992; 150(1)∶1.
3. Ishizuka J, Martinez J, Townsend CM, et al. The effect of gastrin on growth of human stomach cancer cells 〔Ｊ〕. Ann Surg, 1992; 215(5)∶528.
4. 武建国. 实用临床免疫学检验〔Ｍ〕. 第1版. 南京：南京科学技术出版社， 1989∶39.
5. Rana RS, Hokin LE. Role of phosphoinositides in transmembrane signaling 〔Ｊ〕. Physiol Rev, 1990; 70(1)∶115.
6. Ishizuka J, Courtney MT, Richard JB, et al. Effects of gastrin on 3′,5′cyclic adenosine monophosphate, intracellular calcium, and phosphatidylinositol in human colon cancer cells 〔Ｊ〕. Cancer Res, 1994; 54(8)∶2129.
7. 何双梧，谢尚奎，张连阳. 胃泌素对原代培养人大肠癌细胞促增殖作用的研究〔J〕. 中国普外基础与临床杂志， 1998； 5(3)∶143.
8. 吴波，梁念慈. 长春新碱对HL60细胞肌醇磷脂代谢和增殖的影响〔Ｊ〕. 中国药理学通报， 1993； 9(3)∶210.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

EFFECTS AND CLINICAL SIGNIFICANCE OF VINCRISTINE IN GASTRIN-STIMULATING CELL PROLIFERATION ON HUMAN COLONIC CANCER CELL LINE SW480

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