Objective To establish the reliable model of hepatocirrhosis with portal hypertension.
Methods Carbon tetrachloride was subcutaneous injected after devascularization of the circumference of the left renal vein. The morphology of liver, the extrahepatic portal system and portcaval collateral circulation were observed.
Results The liver of rats underwent degeneration and necrosis of hepatocytes, and the normal architecture of the liver lobules was replaced by pseudolobules, which consisted of regenerative hepatocytes and fibrous septa. After 5 weeks, the portal pressure was significantly higher than that of control (Ｐ＜0.05). The diameter of esophageal vein was significantly larger than that of control at 15th week (Ｐ＜0.05).
Conclusion This technique is simple and reliable and can be applied for various experimental studies on portal hypertension.
Citation：
ZHOU Dinghua,YING Dajun,XIAO Lingyun,et al.. MODIFIED PROCEDURE FOR RAT MODEL OF HEPATOCIRRHOSIS WITH PORTAL HYPERTENSION IN RATS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(1): 22-24. doi:
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| 1. |
Mal TC, Groszmann RJ. Pathophysiology of portal hypertension and variceal bleeding 〔Ｊ〕. Surg Clin Am, 1990; 70(2)∶251.
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| 2. |
Sherlock S. The portal venous system and portal hypertension. In: Sherlock S, Dooley JS, editors. Diseases of the liver and biliary system 〔Ｍ〕. 9th ed. Oxford: Blackwell Scientific Publications, 1993; 132-178.
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| 3. |
Tanoue K, Kitano S, Hashizume M, et al. A rat model of esophageal varices 〔Ｊ〕. Hepatology, 1991; 13(3)∶353.
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| 4. |
周丁华， 应大君， 朱楚洪. 复制大鼠门脉高压食管静脉曲张模型的解剖基础 〔Ｊ〕. 解剖学杂志， 1999； 22(2)∶87.
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| 5. |
井口洁. 门脉高压亢进症におげゐ门脉循环の特性と管静脉瘤の成因に关する脏考察 〔Ｊ〕. 肝脏， 1997； 18(5)∶892.
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| 6. |
李增灿. 食管静脉曲张的发生机理及有关问题 〔Ｊ〕. 中华内科杂志， 1993； 32(7)∶843.
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- 1. Mal TC, Groszmann RJ. Pathophysiology of portal hypertension and variceal bleeding 〔Ｊ〕. Surg Clin Am, 1990; 70(2)∶251.
- 2. Sherlock S. The portal venous system and portal hypertension. In: Sherlock S, Dooley JS, editors. Diseases of the liver and biliary system 〔Ｍ〕. 9th ed. Oxford: Blackwell Scientific Publications, 1993; 132-178.
- 3. Tanoue K, Kitano S, Hashizume M, et al. A rat model of esophageal varices 〔Ｊ〕. Hepatology, 1991; 13(3)∶353.
- 4. 周丁华， 应大君， 朱楚洪. 复制大鼠门脉高压食管静脉曲张模型的解剖基础 〔Ｊ〕. 解剖学杂志， 1999； 22(2)∶87.
- 5. 井口洁. 门脉高压亢进症におげゐ门脉循环の特性と管静脉瘤の成因に关する脏考察 〔Ｊ〕. 肝脏， 1997； 18(5)∶892.
- 6. 李增灿. 食管静脉曲张的发生机理及有关问题 〔Ｊ〕. 中华内科杂志， 1993； 32(7)∶843.
