CHANGES OF HDL RECEPTOR AND LDL RECEPTOR ACTIVITY OF HEPATOCYTES DURING CHOLESTEROL GALLSTONE FORMATION IN RABBIT MODEL_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of General Surgery, First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

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Cholesterol gallstone High density lipoprotein receptor Low density lipoprotein receptor Animal experiment

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Objective In order to study the mechanism of cholesterol gallstone formation through rabbit model which was induced by high cholesterol diet (HCD)
Methods the activities of the high density lipoprotein receptor (HDLR) and low density lipoprotein receptor (LDLR) of hepatocytes were investigated.
Results The results were as follows: The HDLR activity increased significantly after taking HCD for one week, at the same time, the LDLR activity only increased slightly. Thereafter, the activities of HDLR and LDLR all decreased markedly. As the time of animals taking HCD went on, serum total cholesterol, LDL cholesterol and hepatic cholesterol increased, but bile acids of biliary tract decreased gradually.
Conclusion The results suggest that the changes of HDLR and LDLR activities of hepatocytes had no significant effect on bile cholesterol and the decreased HDLR and LDLR activities may cause the reduction some of substrate for bile acids synthesise and play an important role in the formation of gallstone.

Citation： SHU Ye,ZHAO Jichun,ZHOU Zongguang,et al.. CHANGES OF HDL RECEPTOR AND LDL RECEPTOR ACTIVITY OF HEPATOCYTES DURING CHOLESTEROL GALLSTONE FORMATION IN RABBIT MODEL. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(2): 72-75. doi: Copy

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11.	Scragg RKR. Plasma lipids and insulin in gallstone disease: a casecontrol study 〔Ｊ〕. Br Med J, 1984; 289(6444)∶521.
12.	Whiting MJ, Wishart RA, Lewis G, et al. Bile acid synthesis by cultural rabbit hepatocytes: stimulation by three lipoprotein fractions 〔Ｊ〕. Biochim Biophys Acta, 1989; 1005(2)∶137.
13.	Halloran LG, Schwartz CC, Vlahcervic ZR, et al. Evidence for highdensity lipoproteinfree cholesterol as the primary precursor for bileacid synthesis in man 〔Ｊ〕. Surg, 1978; 84(1)∶1.
14.	Scobey MW, Johnson FL, Rudel LL. Delivery of high density lipoprotein free and esterfied cholesterol to bile by the perfused monkey liver 〔Ｊ〕. Am J Physiol, 1989; 257(4)∶G644.
15.	Bravo E, Cantafora A. Hepatic uptake and processing of free cholesterol from different lipoproteins with and without sodiumtaurocholate administration: an in vivo study in the rat 〔Ｊ〕. Biochim Biophys Acta, 1990; 1045(1)∶74.

1. 程南生，肖路加，方定志等. 肝细胞膜高密度脂蛋白受体在兔胆红素结石成石过程中的变化〔Ｊ〕. 华西医科大学学报， 1995； 26(1)∶86.
2. 赵纪春，肖路加，舒晔等. 兔胆囊结石形成过程中肝细胞低密度脂蛋白受体变化的研究〔Ｊ〕. 华西医科大学学报， 1998； 29(1)∶42.
3. 别平，孟宪均，杨可祯等. 前列腺素环磷酸腺苷在胆囊结石形成中的作用〔Ｊ〕. 中华外科杂志， 1987； 25(6)∶339.
4. Burstein M, Scholnick HR, Morfin R. Rapid method for the isolation of lipoprotein from human serum by precipitation with polyanions 〔Ｊ〕. J Lipid Res, 1970; 11(6)∶583.
5. 张林华，刘秉文. 一次密度梯度超速离心法分离人血清脂蛋白〔Ｊ〕. 生物化学与生物物理学报， 1989； 21(3)∶257.
6. Brown MS, Goldstein JL. A receptormediated pathway for cholesterol homeostasis 〔Ｊ〕. Science, 1986; 232(1)∶34.
7. Seglen PO. Preparation of rat liver cells 〔Ｊ〕. Exp Cell Res, 1973; 82(2)∶391.
8. Markwell MA, Haas SM, Bieber LL, et al. A modification of the lowry procedure to simplify protein determination in membrane and lipoprotein samples 〔Ｊ〕. Anal Biochem, 1978; 87(1)∶206.
9. 张林华，刘秉文. 肝细胞膜高密度脂蛋白受体的增容及结合特性的研究〔Ｊ〕. 生物化学与生物物理学报， 1991； 18(3)∶255.
10. Eisenberg S. High density lipoprotein metabolism 〔Ｊ〕. J Lipid Res, 1984; 25(10)∶1017.
11. Scragg RKR. Plasma lipids and insulin in gallstone disease: a casecontrol study 〔Ｊ〕. Br Med J, 1984; 289(6444)∶521.
12. Whiting MJ, Wishart RA, Lewis G, et al. Bile acid synthesis by cultural rabbit hepatocytes: stimulation by three lipoprotein fractions 〔Ｊ〕. Biochim Biophys Acta, 1989; 1005(2)∶137.
13. Halloran LG, Schwartz CC, Vlahcervic ZR, et al. Evidence for highdensity lipoproteinfree cholesterol as the primary precursor for bileacid synthesis in man 〔Ｊ〕. Surg, 1978; 84(1)∶1.
14. Scobey MW, Johnson FL, Rudel LL. Delivery of high density lipoprotein free and esterfied cholesterol to bile by the perfused monkey liver 〔Ｊ〕. Am J Physiol, 1989; 257(4)∶G644.
15. Bravo E, Cantafora A. Hepatic uptake and processing of free cholesterol from different lipoproteins with and without sodiumtaurocholate administration: an in vivo study in the rat 〔Ｊ〕. Biochim Biophys Acta, 1990; 1045(1)∶74.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

CHANGES OF HDL RECEPTOR AND LDL RECEPTOR ACTIVITY OF HEPATOCYTES DURING CHOLESTEROL GALLSTONE FORMATION IN RABBIT MODEL

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