SEPARATION OF IMMUNE PRIVILEGE TESTICULAR CELL AND EXPRESSION OF FAS LIGAND_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of Surgery, First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

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Testicular Immune privilege Fas ligand

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Objective To investigate the rationale of immune privilege of testicular sertoli cell.
Methods Testicular sertoli cell was prepared by digested collagenase, trypsin, and Dnase. In vitro, the sertoli cells were culture together with active lymphocytes to observe the effect on killing lymphocytes. SABC was used for labeling the Fas ligand on testicular sertoli cell.
Results In vitro, sertoli cell can kill the active lymphocytes, and testicular sertoli cell expresses the Fas ligand. Conclusion Fas ligand expressing on the testicular sertoli cell may be the cause of immune privilege of testicular.

Citation： LAN Ping,ZHANG Mei,GUAN Peng,et al.. SEPARATION OF IMMUNE PRIVILEGE TESTICULAR CELL AND EXPRESSION OF FAS LIGAND. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(2): 83-84下转. doi: Copy

1.	Medawar PB. Immunity to homologous grafted skin Ⅲ in the fate of skin homografts transplanted to the brain to subcutaneous tissue, and to the anterior 〔Ｊ〕. Br J Exp Pathol, 1948; 29(1)∶58.
2.	Neaves WB, Billingham RE. The lymphatic drainage of the rat prostate and its status as an immunologically privileged site 〔Ｊ〕. Transplantation, 1979; 27(1)∶127.
3.	Korbutt GS, Elliott JF, Rojotte R. Cotransplantation of allogeneic islets with allogeneic testicular cell aggregates allows longterm graft survival without systemic immunosuppression 〔Ｊ〕. Diabetes, 1997; 46(2)∶317.
4.	Griffith TS, Brunner J, Fletcher SM. Fas ligandinduced apoptosis as a mechanism of immune privilege 〔Ｊ〕. Science, 1995; 270(17)∶1189.
5.	Selawry HP, Cameron DF. Sertoli cellenriched fractions in successful islet cell transplantation 〔Ｊ〕. Cell Transplantation, 1993; 2(1)∶123.
6.	Nagata S, Golstein P. The Fas death factor 〔Ｊ〕. Science, 1995; 267(5203)∶1449.
7.	Selaway HP, Kotb M, Herrod HG, et al. Production of a factor, or factors, suppressing IL2 production and Tcell proliferation by sertoli cellenriched preparations: a potention role for islet transplantation in an immunologically privileged site 〔Ｊ〕. Transplantation, 1991; 52(5)∶846.
8.	Bellgrau D, Dild D, Selawry H. A role for CD95 ligand in preventing graft rejection 〔Ｊ〕. Nature, 1995; 377(6550)∶630.
9.	Selawry HP, Cameron DF. Sertoli cellenriched fractions in successful islet cell transplantation 〔Ｊ〕. Cell Transplantation, 1993; 2∶123.

1. Medawar PB. Immunity to homologous grafted skin Ⅲ in the fate of skin homografts transplanted to the brain to subcutaneous tissue, and to the anterior 〔Ｊ〕. Br J Exp Pathol, 1948; 29(1)∶58.
2. Neaves WB, Billingham RE. The lymphatic drainage of the rat prostate and its status as an immunologically privileged site 〔Ｊ〕. Transplantation, 1979; 27(1)∶127.
3. Korbutt GS, Elliott JF, Rojotte R. Cotransplantation of allogeneic islets with allogeneic testicular cell aggregates allows longterm graft survival without systemic immunosuppression 〔Ｊ〕. Diabetes, 1997; 46(2)∶317.
4. Griffith TS, Brunner J, Fletcher SM. Fas ligandinduced apoptosis as a mechanism of immune privilege 〔Ｊ〕. Science, 1995; 270(17)∶1189.
5. Selawry HP, Cameron DF. Sertoli cellenriched fractions in successful islet cell transplantation 〔Ｊ〕. Cell Transplantation, 1993; 2(1)∶123.
6. Nagata S, Golstein P. The Fas death factor 〔Ｊ〕. Science, 1995; 267(5203)∶1449.
7. Selaway HP, Kotb M, Herrod HG, et al. Production of a factor, or factors, suppressing IL2 production and Tcell proliferation by sertoli cellenriched preparations: a potention role for islet transplantation in an immunologically privileged site 〔Ｊ〕. Transplantation, 1991; 52(5)∶846.
8. Bellgrau D, Dild D, Selawry H. A role for CD95 ligand in preventing graft rejection 〔Ｊ〕. Nature, 1995; 377(6550)∶630.
9. Selawry HP, Cameron DF. Sertoli cellenriched fractions in successful islet cell transplantation 〔Ｊ〕. Cell Transplantation, 1993; 2∶123.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

SEPARATION OF IMMUNE PRIVILEGE TESTICULAR CELL AND EXPRESSION OF FAS LIGAND

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