Objective To probe the change of the structure and function of the small bowel by injection of different drugs (verapamil, energy compounds or normal saline) via the superior mesenteric artery (SMA) injections.
Methods The model of the small intestine ischemia/reperfusion (I/R) injury was made in grey rabbits. Free calcium concentration in mitochondria of the small intestine was determined, and the ultrastructural change was also observed by electron microscopy at the very time of occlusion, 60 minutes after occlusion and 30 minutes after reperfusion.
Results The free calcium concentration in mitochondria was more declined in verapamil group (2.976±0.410 nmol/mg.prot) than in N.S. group (4.234±0.542 nmol/mg.prot), Ｐ＜0.01, at 60 minutes after occlusion. At 30 minutes after reperfusion, free calcium concentration in mitochondria was more decreased in energy compunds group (2.401±0.323 nmol/mg.prot) and verapamil group (3.847±0.610 nmol/mg.prot) than in the N.S. group (5.981±1.031 nmol/mg.prot).
Conclusion Verapamil and energy compouds have protective effects on the functions and ultrastructures of the I/R of small intestine.
Citation：
CHEN Guoy,NI Shaozhong.. EFFECTS OF VERAPAMIL AND ENERGY COMPOUNDS ON STRUCTURAL AND FUNCTIONAL CHANGES OF SMALL BOWEL′S IN RABBITS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(3): 135-137. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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Chaudry IH, Ohkawa M, Clemens MG. Improved mitochondrial function following ischemia and reflow by ATPMgCl2 〔Ｊ〕. Am J Physiol, 1984; 246(3)∶R799.
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O′Brien PJ, Li Go, Loche M. Compensatory up regulation of cardiac SR Ca2+pump by heatshock counteracts SR Ca2+channel activation by ischemia/reperfusion 〔Ｊ〕. Mol Cell Biochem, 1997; 73(2)∶135.
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Kang Zhao, Levin SS. Correlation of I/R or partial outlet obstructioninduced spectrin proteolysis by calpain with contractile dysfunction in rabbit bladder 〔Ｊ〕. Urology, 1997; 49(4)∶293.
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Tani M, Neely JR. Role of intracellular Na+ in Ca2+ overload and depressed recovery of ventricular function of reperfused ischemic rat hearts: possible involvement of H+Na+ and Na+Ca2+ exchange 〔Ｊ〕. Circ Res, 1989; 65(6)∶1045.
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Granger DN, Mc Cord JM. Xanthine oxidase inhibitors attenuate ischemiainduced vascular permeability changes in the cat intestine 〔Ｊ〕. Gastroenterology, 1986; 90(1)∶80.
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| 7. |
Massberg S, Leiderer R. Carolina rinse attenuates postischemic microvascular injury in rat small bowel isografts 〔Ｊ〕. Surgery, 1998; 123(2)∶181.
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- 1. Chaudry IH, Ohkawa M, Clemens MG. Improved mitochondrial function following ischemia and reflow by ATPMgCl2 〔Ｊ〕. Am J Physiol, 1984; 246(3)∶R799.
- 2. O′Brien PJ, Li Go, Loche M. Compensatory up regulation of cardiac SR Ca2+pump by heatshock counteracts SR Ca2+channel activation by ischemia/reperfusion 〔Ｊ〕. Mol Cell Biochem, 1997; 73(2)∶135.
- 3. Burke TJ, Amold PE, Gordon JA. Protective effect of intrarenal calcium membrane blockers before or after renal ischemia 〔Ｊ〕. J Clin Invest, 1984; 74(4)∶1830.
- 4. Kang Zhao, Levin SS. Correlation of I/R or partial outlet obstructioninduced spectrin proteolysis by calpain with contractile dysfunction in rabbit bladder 〔Ｊ〕. Urology, 1997; 49(4)∶293.
- 5. Tani M, Neely JR. Role of intracellular Na+ in Ca2+ overload and depressed recovery of ventricular function of reperfused ischemic rat hearts: possible involvement of H+Na+ and Na+Ca2+ exchange 〔Ｊ〕. Circ Res, 1989; 65(6)∶1045.
- 6. Granger DN, Mc Cord JM. Xanthine oxidase inhibitors attenuate ischemiainduced vascular permeability changes in the cat intestine 〔Ｊ〕. Gastroenterology, 1986; 90(1)∶80.
- 7. Massberg S, Leiderer R. Carolina rinse attenuates postischemic microvascular injury in rat small bowel isografts 〔Ｊ〕. Surgery, 1998; 123(2)∶181.
