AMPLIFICATION OF CYCLIND1 GENE AND OVEREXPRESSION OF ITS PROTEIN PRODUCT IN BREAST CANCER_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHAO Junling , YANG Jingqiao , LAI Yi.

Department of General Surgery, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

Corresponding author：

Keywords：

Breast cancer CyclinD1 gene CyclinD1 protein

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Objective To investigate the clinical significance of the amplification of CyclinD1 gene and overexpression of its protein product in breast cancer.
Methods Semi-quantitative PCR and immunohistochemical techniques were used to examine the amplification of CyclinD1 gene and overexpression of its protein product in breast cancer, mammary tissues adjacent to the tumor, mammary benign disease and normal mammary tissues.
Results The results showed that amplification of CyclinD1 gene was observed in 14 out of 62 (22.6%) breast cancer, the overexpression of its protein product was 48.4% (30/62). Compared with other mammary tissues, these findings were significant. Amplification of CyclinD1 gene and overexpression of its protein were positively associated with histological grade, and there was a b correlation between CyclinD1 protein overexpression and estrogen receptor (progestogen receptor).
Conclusion The degree of CyclinD1 protein overexpression is not quite the same as the frequency of the amplification of CyclinD1 gene. It suggests that the overexpresstion of CyclinD1 protein is also attributed to some other mechanisms. Estrogen might stimulate the expression of CyclinD1. The detection of CyclinD1 gene and its protein in breast cancer has some clinical significances.

Citation： ZHAO Junling,YANG Jingqiao,LAI Yi.. AMPLIFICATION OF CYCLIND1 GENE AND OVEREXPRESSION OF ITS PROTEIN PRODUCT IN BREAST CANCER. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(3): 147-149下转163. doi: Copy

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2.	Kyomoto R, Kumazwa H, Toda Y, et al. CyclinD1gene amplification is a more potent prognostic factor than its protein overexpression in human headandneck squamouscell carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶576.
3.	Bartkova J, Lukas J, Müller H, et al. CyclinD1 protein expression and function in human breast cancer 〔Ｊ〕. Int J Cancer, 1994; 57(1)∶353.
4.	Michalides R, Hageman P, Tinteren H, et al. A clinicopathological study on overexpression of CyclinD1 and of p53 in a series of 248 patients with operable breast cancer 〔Ｊ〕. Br J Cancer, 1996; 73(2)∶728.
5.	Paul J, Michalides R, Jannink I, et al. CyclinD1 expression in invasive breast cancer 〔Ｊ〕. Am J Pathology, 1997; 150(2)∶705.
6.	Zhang SY, Caamano J, Cooper F, et al. Immunohistochemistry of CyclinD1 in human breast cancer 〔Ｊ〕. Am J Clin Pathol, 1994; 102(2)∶695.
7.	沈凯，唐孝达，杨宇如. D类细胞周期素与癌基因和抑癌基因〔Ｊ〕. 中华医学遗传学杂志， 1996； 13(6)∶361.
8.	Surmacz E, Reiss K, Sell C, et al. CyclinD1 messenger RNA is inducible by plateletderived growth factor in culture fibroblasts 〔Ｊ〕. Cancer Res, 1992; 52(19)∶4522.
9.	Furlanetto RW, Harwell SE, Firck KK. Insulinlike growth factor1 induces CyclinD1 expression in MG 63 human osteosarcoma cell in vitro 〔Ｊ〕. Mol Endocrinol, 1994; 8(2)∶510.
10.	Surmacz E, Marina A, Mary K, et al. Type I insulinlike growth factor receptor function in breast cancer 〔Ｊ〕. Breast Cancer Research and Treatment, 1998; 47(1)∶255.

1. Kamb A, Gruis NA, Weaver FT, et al. A cell cycle regulator potentially in genesis of many tumor types 〔Ｊ〕. Science, 1994; 264(2)∶436.
2. Kyomoto R, Kumazwa H, Toda Y, et al. CyclinD1gene amplification is a more potent prognostic factor than its protein overexpression in human headandneck squamouscell carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶576.
3. Bartkova J, Lukas J, Müller H, et al. CyclinD1 protein expression and function in human breast cancer 〔Ｊ〕. Int J Cancer, 1994; 57(1)∶353.
4. Michalides R, Hageman P, Tinteren H, et al. A clinicopathological study on overexpression of CyclinD1 and of p53 in a series of 248 patients with operable breast cancer 〔Ｊ〕. Br J Cancer, 1996; 73(2)∶728.
5. Paul J, Michalides R, Jannink I, et al. CyclinD1 expression in invasive breast cancer 〔Ｊ〕. Am J Pathology, 1997; 150(2)∶705.
6. Zhang SY, Caamano J, Cooper F, et al. Immunohistochemistry of CyclinD1 in human breast cancer 〔Ｊ〕. Am J Clin Pathol, 1994; 102(2)∶695.
7. 沈凯，唐孝达，杨宇如. D类细胞周期素与癌基因和抑癌基因〔Ｊ〕. 中华医学遗传学杂志， 1996； 13(6)∶361.
8. Surmacz E, Reiss K, Sell C, et al. CyclinD1 messenger RNA is inducible by plateletderived growth factor in culture fibroblasts 〔Ｊ〕. Cancer Res, 1992; 52(19)∶4522.
9. Furlanetto RW, Harwell SE, Firck KK. Insulinlike growth factor1 induces CyclinD1 expression in MG 63 human osteosarcoma cell in vitro 〔Ｊ〕. Mol Endocrinol, 1994; 8(2)∶510.
10. Surmacz E, Marina A, Mary K, et al. Type I insulinlike growth factor receptor function in breast cancer 〔Ｊ〕. Breast Cancer Research and Treatment, 1998; 47(1)∶255.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

AMPLIFICATION OF CYCLIND1 GENE AND OVEREXPRESSION OF ITS PROTEIN PRODUCT IN BREAST CANCER

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