EFFECT OF INTERLEUKIN 10 ON INHIBITION OF CYTOKINE RELEASE IN ABDOMINAL INFECTION AND EARLY SEPSIS OF RAT CAN EXPERIMENTAL STUDY_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Zuyao , HU Tingze , WEI Fukang , et al.

Department of Pediatric Surgery, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

Corresponding author：

Keywords：

Interleukin 10 Sepsis Rat Inflammatory cytokine

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the effect of IL-10 on the inhibition of early proinflammatory cytokine release in intraabdominal infection and early sepsis.
Methods Forty eight SD rats were randomized into 4 groups, 12 in each group, ①sham operation group, ②control group, ③prophylactic group, ④therapeutic group. Group 1 underwent laparotomy only, group 2 received laparotomy and cecal ligation plus punctures (CLP) with saline injected once every 3 hrs, group 3 underwent CLP and IL-10 injection intraperitoneally 1 hr before surgery and once every 3 hrs following operation, group 4 received CLP and IL-10 injection once every 3 hrs after operation. At 3 and 9 hr points, rats were sacrificed and blood samples were taken for measurement of inflammatory cytokines.
Results Almost no inflammatory cytokines were detected in sham group, CLP produced a significant rise in serum TNF-α (tumor necrosis α), IL-1, IL-6 (interleukin 1,6) in control group, IL-10 reduced the rise of inflammatory cytokines significantly.
Conclusion IL-10 could inhibit the early inflammatory cytokine release in rat model of sepsis. Suggesting it may attenuate the severity of inflammation.

Citation： WANG Zuyao,HU Tingze,WEI Fukang,et al.. EFFECT OF INTERLEUKIN 10 ON INHIBITION OF CYTOKINE RELEASE IN ABDOMINAL INFECTION AND EARLY SEPSIS OF RAT CAN EXPERIMENTAL STUDY. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(5): 291-292下转294. doi: Copy

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1. Howard M, Muchamuel T, Andrades S, et al. Interleukin 10 protects mice from lethal endotoxemia 〔Ｊ〕. Exp Med, 1993; 177(4)∶1205.
2. Wichterman KA, Baue AE, Chaudry H. Sepsis and septic shock: a review of laboratory models and a proposal 〔Ｊ〕. Surg Res, 1980; 29(2)∶189.
3. Baker CC, Chaudry IH. Evaluation of factors affecting mortalituy rate after sepsis in a murine cecal ligation and puncture model 〔Ｊ〕. Surgery, 1992; 94(2)∶331.
4. Rongione AJ, Kusske AM, Ashley SW, et al. Interleukin 10 reduces the severity of acute pancreatitis in rats 〔Ｊ〕. Gastroenterology, 1997; 112(3)∶960.
5. Elliott J, Mosmann T. IL10 inhibits cytokine production, vascular leakage and swelling during Thelper cellinduced delayedtype hypersensitivity 〔Ｊ〕. Immunol, 1994; 153(9)∶3967.
6. Dinarello CA, Galfand JA. Anticytokine strategies in the treatment of the systemic inflammatory response syndrome 〔Ｊ〕. JAMA, 1993; 269(14)∶1829.
7. Remick DG, Gard SJ, Newcomb DE, et al. Exogenous interleukin10 fails to decrease the mortality or morbidity of sepsis 〔Ｊ〕. Crit Care Med, 1998; 26J(5)∶895.
8. Moore KW, O’Garra A. Interleukin10 〔Ｊ〕. Annu Rew Immunol, 1993; 11(4)∶165.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

EFFECT OF INTERLEUKIN 10 ON INHIBITION OF CYTOKINE RELEASE IN ABDOMINAL INFECTION AND EARLY SEPSIS OF RAT CAN EXPERIMENTAL STUDY

Abstract Full text Figures/Tables Video References Cited by

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