• Department of Respiratory Medicine, West China Hospital,Sichuan University.Chengdu,Sichuan,610041,China Corresponding author:FAN Hong,E-mail:fanhongfan@yahoo.com;
FAN Hong, Email: fanhongfan@yahoo.com
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Objective  To observe the effects of hydrogen peroxide on the expression of transforming growth factorβ1(TGF-β1) and Smad3 protein in A549 cells. Methods  A549 cells were cultured with different concentrations of hydrogen peroxide. MTT assay was used to determine the cell growth and survival rates. The level of TGF-β1 and p-Smad3 protein were detected by western blotting. Results  It was observed that hydrogen peroxide significantly inhibit proliferation of A549 cells. When the concentration of hydrogen peroxide was 1.0 mmol/L, the inhibition ratio reaches 46.34%, and the level of TGF-β1 and p-Smad3 protein were increased in a time-dependence manner and reached a peak after 24 h, then decreased a little but also remained at high level. Conclusions  In the early oxidative damage, A549 cells express high level of TGF-β1 and p-Smad3 protein. It may be relevant to tissue repair and remodeling after lung injury.

Citation: YANG Dongmei,DING Yu,YING Binwu,JIANG Fei,XU Dan,MEILANG QUCUO,FAN Hong. Effects of hydrogen peroxide on expression of transforming growth factor-β1 and Smad3 in A549 cells. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(1): 43-46. doi: Copy

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